Rapid Construction of a Replication-Competent Infectious Clone of Human Adenovirus Type 14 by Gibson Assembly [PDF]
In 1955, Human adenovirus type 14 (HAdV-B14p) was firstly identified in a military trainee diagnosed as acute respiratory disease (ARD) in the Netherlands. Fifty years later, a genomic variant, HAdV-B14p1, re-emerged in the U.S.
Haibin Pan +13 more
doaj +3 more sources
Comparison of chikungunya viruses generated using infectious clone or the Infectious Subgenomic Amplicons (ISA) method in Aedes mosquitoes. [PDF]
Reverse genetics systems provide the opportunity to manipulate viral genomes and have been widely used to study RNA viruses and to develop new antiviral compounds and vaccine strategies.
Souand Mohamed Ali +5 more
doaj +3 more sources
Construction and characterization of an infectious clone of coxsackievirus A16 [PDF]
Background Coxsackievirus A16 (CVA16) is a member of the Enterovirus genus of the Picornaviridae family and it is a major etiological agent of hand, foot, and mouth disease (HFMD), which is a common illness affecting children.
Liu Fei +4 more
doaj +2 more sources
Construction of an infectious clone of novel duck picornavirus using the infectious subgenomic amplicon method [PDF]
A picornavirus strain of Duck/FC22/China/2017 (FC22) with an unclear taxonomic status was isolated in our laboratory in 2017. To study the biological properties of the virus, an infectious clone of the FC22 strain was successfully rescued by the ...
Jitong Li +10 more
doaj +2 more sources
A maize non- infectious clone from Sugarcane mosaic virus can move in detached tobacco leaves [PDF]
Background: Infectious clones are copies of a virus genome produced in vitro or inside a vector and can infect inoculated healthy plants or cells. They are important tools to study the molecular biology of viruses.
Gustavo Rodriguez-Gomez +1 more
doaj +2 more sources
Construction and modification of a low-copy plasmid-based infectious clone for GI-19 genotype IBV via Red/ET recombineering: A simplified and efficient reverse genetics system for coronavirus [PDF]
Avian infectious bronchitis virus (IBV), a Gamma coronavirus (γ-CoV), is one of the most economically important pathogens affecting the global poultry industry.
Keyu Feng +6 more
doaj +2 more sources
Development of a Full-Length Infectious cDNA Clone of the Grapevine Berry Inner Necrosis Virus
Grapevine berry inner necrosis virus (GINV) belongs to the genus Trichovirus in the family Betaflexiviridae. The GINV isolate LN_BETA_RS was obtained from a “Beta” grapevine (Vitis riparia × Vitis labrusca) exhibiting chlorotic mottling and ring spot in ...
Xudong Fan +6 more
doaj +3 more sources
Development and utilization of an infectious clone for porcine deltacoronavirus strain USA/IL/2014/026 [PDF]
Kelly Lager +2 more
exaly +2 more sources
Construction of a Novel Infectious Clone of Recombinant Herpesvirus of Turkey Fc-126 Expressing VP2 of IBDV [PDF]
Jichun Wang, Yating Zheng, Mengwei Xu
exaly +2 more sources
The Construction and Characterization of an Infectious Clone of the Asian Genotype Chikungunya Virus from Yunnan, China. [PDF]
Chikungunya fever (CHIKF), which is caused by the Chikungunya virus (CHIKV), has rapidly spread across the globe in recent years, leading to its listing as a public health concern by the World Health Organization.
Ning X +6 more
europepmc +2 more sources

