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A guide to the contained use of plant virus infectious clones [PDF]
Plant Biotechnology Journal, 2018 AbstractPlant virus infectious clones are important tools with wide‐ranging applications in different areas of biology and medicine. Their uses in plant pathology include the study of plant–virus interactions, and screening of germplasm as part of prebreeding programmes for virus resistance.
Diane L Hird +2 more
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Construction of an infectious cDNA clone of echovirus 6
Virus Research, 1992A complete cDNA copy of the echovirus 6 genome was constructed. Complementary DNA was reversed transcribed from viral RNA. Subgenomic cDNAs were obtained by direct cloning and polymerase chain reactions. Full length cDNA was constructed into the Bluescript II vector (pBSII) using unique, overlapping, restriction sites of four clones.
R V, Blackburn +2 more
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Studying Poliovirus with Infectious Cloned cDNA
Clinical Infectious Diseases, 1984The application of molecular cloning to the study of poliovirus is discussed. Initially cDNA clones representing the entire genome of poliovirus type 1 (Mahoney) were prepared. These molecular clones were used for determining the complete nucleotide sequence of the viral genome. Subsequently a single cDNA clone representing the entire poliovirus genome
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Infectious cDNA clones of two grapevine viruses
Archives of Virology, 2000Full-length cDNA copies of the genomes of Grapevine virus A (GVA) and Grapevine virus B (GVB) under the control of bacteriophage T7 promoter have been synthesized, which were refractory to cloning in Escherichia coli. However, both transcribed cDNAs were infectious when mechanically inoculated to Nicotiana plants.
P, Saldarelli, M, Dell'Orco, A, Minafra
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Construction of an infectious dahlia common mosaic virus clone
Archives of Virology, 2021Dahlia is a major ornamental plant that is cultivated worldwide. However, dahlia plants, which are mainly propagated through vegetative reproduction, are susceptible to widespread damage by viruses, and viral control requires that the nature of the infecting virus(es) be known. In this study, dahlia common mosaic virus (DCMV) was detected for the first
Daiki Yamamoto +4 more
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A strategy for cloning infectious molecular clones of retroviruses from serum or plasma
Journal of Virological Methods, 2000To enable biological characterisation of lentiviral variants which emerge during infection and development of AIDS, a method was developed to construct molecular clones from circulating simian immunodeficiency virus (SIV) particles present in as little as 20 microl of serum from infected rhesus monkeys. This technique uses a long distance RT-PCR method
L, Holterman +4 more
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Cloned Poliovirus Complementary DNA Is Infectious in Mammalian Cells
Science, 1981A complete, cloned complementary DNA copy of the RNA genome of poliovirus was constructed in the Pst I site of the bacterial plasmid pBR322. Cultured mammalian cells transfected with this hybrid plasmid produced infectious poliovirus. Cells transfected with a plasmid which lacked the first 115 bases of the poliovirus genome did not produce virus.
V R, Racaniello, D, Baltimore
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Biolistic Inoculation of Plants with Geminivirus Infectious Clones
The propagation of viruses in laboratory-controlled conditions is an essential step in studying viruses' molecular and biological properties. For geminiviruses, the main methods of inoculation are agroinoculation and biolistics. Here, we describe the protocol for inoculating self-ligated cloned geminivirus inserts into plants using particle bombardment.Cristiano, Lacorte +2 more
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A plant intron enhances the performance of an infectious clone in planta
Journal of Virological Methods, 2019Although infectious clones are fundamental tools in virology and plant pathology, their efficacy is often reduced by the instability of viral sequences in Escherichia coli. In this study, we constructed an infectious clone of PepMoV (pPepMoV) in a bacterial binary vector (pSNU1); the clone induces symptoms of PepMoV in agroinfiltrated plants.
Phu-Tri Tran +3 more
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Construction of Infectious Clones for DNA Viruses: Mastreviruses
2008To characterize a virus at the molecular and biological levels, it is necessary to produce an infectious clone. For most of the Geminiviridae, cloning of the genome is relatively easy because of their small genomes and the presence of the virus double-stranded (replicative) DNA form in infected plants.
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