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Construction of Infectious Clones for RNA Viruses: TMV
2008The generation of infectious clones is routinely the first step for reverse genetic studies of RNA plant virus gene and sequence function. The procedure given here, details the creation of cDNA clones of tobacco mosaic virus, from which infectious transcripts can be generated in vitro with T7 RNA polymerase.
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Construction of an infectious clone of human adenovirus type 41
Archives of Virology, 2012Human adenovirus type 41 (HAdV-41) is well known for its fastidiousness in cell culture. To construct an infectious clone of HAdV-41, a DNA fragment containing the left and right ends of HAdV-41 as well as a kanamycin resistance gene and a pBR322 replication origin was excised from the previously constructed plasmid pAd41-GFP.
Duo-Ling, Chen +7 more
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[Rapid construction of infectious clones of infectious bursal disease virus].
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica, 2003A rapid procedure was established for rescuing infectious bursal disease virus (IBDV), an important pathogen in poultry. A full-length cDNA clone of the segment B of a CEF-adapted IBDV strain HZ2 was constructed by long RT-PCR, and the 2827 bp nucleotide sequence, including the 5 - and 3 -noncoding regions (NCR), was established. Then the cDNA clone of
Yao-Wei, Huang +3 more
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Generating West Nile Virus from an Infectious Clone
2016WNV infectious clones are valuable tools for elucidating WNV biology. Nevertheless, relatively few infectious WNV clones have been generated because their construction is hampered by the instability of flaviviral genomes. More recently, advances in cloning techniques as well as the development of several two-plasmid WNV infectious clone systems have ...
Rianna, Vandergaast +1 more
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Development of Infectious Clone of Virus
2020Alangar Ishwara Bhat, Govind Pratap Rao
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Construction of full-length cDNA infectious clones of Chilli veinal mottle virus
Virus Research, 2022Dehui Xi, Qiding Peng
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Development and characterization of infectious clones of two strains of Usutu virus
Virology, 2021Seth A Hawks +2 more
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A plant intron enhances the performance of an infectious clone
Journal of Virological Methods, 2019Kook-Hyung Kim +2 more
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