Results 111 to 120 of about 2,456,442 (305)

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

Drug Interactions of Imperatorin and Curcumin on Macitentan in vitro and in vivo

Drug Design, Development and Therapy
Hualu Wu,1 Qian Liu,2 Yuxin Shen,1 He Qi,3 Jiade Zhao,3 Qiaoying Li,4 Hailun Xia,1 Ren-Ai Xu,1 Lu Shi1 1Department of Pharmacy, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, People’s Republic of China; 2Department of ...
Wu H, Liu Q, Shen Y, Qi H, Zhao J, Li Q, Xia H, Xu RA, Shi L   +8 more
doaj  

CCm performance in Arctic and Antarctic seaweeds in a warming and acidifying marine environment [PDF]

, 2016
Presentación oral al congresoOcean acidification and warming are affecting with special intensity the polar coastal ecosystems. The Arctic kelps Saccharina latissima and Laminaria solidungula from Kongsfjorden (Svalbard) were cultured at 4 and 9 ºC in ...
Gordillo, Francisco JL, Heinrich, Sandra, Iiguez, Concepción   +2 more
core  

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

Porcine serum maltase-glucoamylase: structure, kinetics, and inhibition

Journal of Enzyme Inhibition and Medicinal Chemistry
Maltase-glucoamylase (MGAM) is a small-intestinal enzyme comprising two tandem α-glucosidase units, NtMGAM and CtMGAM, each capable of hydrolysing maltodextrins into glucose.
Ken Watanabe, Takayoshi Tagami, Chihiro Biwa, Masato Kawasaki, Naruhiko Adachi, Toshio Moriya, Toshiya Senda, Masayuki Okuyama   +7 more
doaj   +1 more source

Linking neurogenesis, oligodendrogenesis, and myelination defects to neurodevelopmental disruption in primary mitochondrial disorders

FEBS Letters, EarlyView.
Mitochondrial remodeling shapes neural and glial lineage progression by matching metabolic supply with demand. Elevated OXPHOS supports differentiation and myelin formation, while myelin compaction lowers mitochondrial dependence, revealing mitochondria as key drivers of developmental energy adaptation.
Sahitya Ranjan Biswas, Porter L. Tomsick, Alicia M. Pickrell, Paul D. Morton   +3 more
wiley   +1 more source

Study on the Inhibitory Mechanisms of Native Cellulose, Microcrystalline Cellulose, and Soluble Cellodextrin on α-Amylase and Amyloglucosidase

Foods
The inhibitory effects of natural cellulose (NC), microcrystalline cellulose (MC), and soluble fiber dextrin (SC) on amylase activity have been established; however, the underlying mechanisms remain poorly understood.
Yanli Zhu, Lin Su, Shanshan Liu, Liping Lu, Li Song, Huimin Ma, Mingyue Zhang, Dandan Gao   +7 more
doaj   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source