Results 241 to 250 of about 7,131,135 (287)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Comparative Investigation of Red Wine Concentrates as Multifunctional Skin-Protective Agents. [PDF]

Pharmaceutics
Ilić G, Beara I, Milovanović L, Jovanović A, Dekanski D, Pirković A.   +5 more
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus

FEBS Open Bio, EarlyView.
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima, Gustavo Narvaes Guimarães, Emilyane de Oliveira Santana Amaral, Bianca Caroline Figueiredo Bianco, Manuel Jara‐Espejo, Sérgio Roberto Peres Line, Ana Paula de Souza, Aline Cristiane Planello   +7 more
wiley   +1 more source

Discovery of Ro 41-0960, a Non-Steroidal Inhibitor of the Na⁺/K⁺-ATPase. [PDF]

ChemMedChem
Cruz-Cortés C, Šeflová J, Guerrero-Serna G, Jimenez-Vazquez EN, Anumonwo J, Espinoza-Fonseca LM.   +5 more
europepmc   +1 more source

Silver Nanoparticles in Donkey Semen Stored at 17°C: Effect on Sperm Parameters and Bacterial Growth. [PDF]

Reprod Domest Anim
Aguilera VP, Vallejo-Soto P, Montero-Palma C, Vilela B, Romero-Salmoral A, Galán-Relaño Á, Crespo-Castejón F, González-Ariza A, Hidalgo M, Dorado J, Ortiz I, Gómez-Gascón L, Luque I.   +12 more
europepmc   +1 more source

Hydrolysis-Driven Compositional Modification of Flavonoids in the Aerial Parts of <i>Lespedeza cyrtobotrya</i> Miq. and Its Impact on Monoamine Oxidase A Inhibitory Activity. [PDF]

ACS Omega
Kim HG, Lee SA, Kim JH, Lee SY, Ahn J, Oh SR, Lee D, Ko SK, Ryu HW.   +8 more
europepmc   +1 more source

Can Fluconazole Be Used to Treat Non-Resistant Candida (Candidozyma) auris Infections? Preclinical Pharmacokinetic/Pharmacodynamic Data From a Galleria mellonella Infection Model. [PDF]

J Infect Dis
Kroustali V, Pournaras S, Meletiadis J.
europepmc   +1 more source