Results 71 to 80 of about 378,797 (150)
Untangling Inositol (Pyro)Phosphate Biology Through Emerging Technologies
Inositol (pyro)phosphates represent a vital class of intracellular messengers that govern multiple biological processes. The last decade has experienced a surge of interest in the functions of this class of signaling molecules, primarily due to the introduction of several innovative analytical technologies.
Adolfo Saiardi +5 more
wiley +1 more source
Thermococcus thioreducens\u27 Inorganic Pyrophosphatase Purification [PDF]
Protein purification requires an intensive multi-step process that is customized to every protein’s characteristic for optimal results. In this study, we looked at the purification process of Inorganic Pyrophosphatase (IPPase), an essential protein found
Nozum, Christopher, Sleiman, Zeina
core +1 more source
Inspired by the role of Mg2+ in stabilizing RNA secondary structures, we developed a one‐pot biomimetic mineralization method for synthesizing a circular RNA‐based nanovaccine (circRNA@FAiNVac) that integrates formulation and adjuvant functions. This nanovaccine exhibits potent immunostimulatory activation enabling multimodal immune responses and ...
Liangru Lin +10 more
wiley +1 more source
Bacillus subtilis Mobilizes Phosphorus by Reshaping P Pools, Sorption, and Enzyme Activities
ABSTRACT Low phosphorus (P) availability constrains crop production in saline‐alkaline soils of Xinjiang, China. We tested whether Bacillus subtilis ( B. subtilis ) mobilizes soil P by altering P fractions, sorption–desorption, and P‐cycling enzymes in a saline‐alkaline cotton soil. Under conventional fertilization, B.
Haiping Xie +5 more
wiley +1 more source
Proton pumping inorganic pyrophosphatase of endoplasmic reticulum-enriched vesicles from etiolated mung bean seedlings [PDF]
[[abstract]]Endoplasmic reticulum (ER)-enriched vesicles from etiolated hypocotyls of mung bean seedlings (Vigna radiata) were successfully isolated using Ficoll gradient and two-phase (polyethylene glycol-dextran) partition.
Kuo SY; Chien LF; Hsiao YY; Van RC; Yan KH; Liu PF; Mao SJT
core
Purification and pyrophosphatase activity of PHP domain.
A) SDS-PAGE of fractions collected during the purification of the E. coli PHP domain. M: molecular mass markers. Lane 1: soluble proteins isolated after refolding of the inclusion bodies containing PHP; lanes 2 and 3: purified PHP domain after ion ...
Massimiliano Lanzi (1463971) +7 more
core +1 more source
SPATIAL STRUCTURE OF INORGANIC PYROPHOSPHATASE ESCHERICHIA COLI AT RESOLUTION 2.2A
The work covers the crystals of the enzyme in the inorganic pyrophosphatase Escherichia coli. The aim of the work is to determine the atomic structure of the inorganic pyrophosphatase enzyme. The atomic structure of the object has been determined firstly;
Oganesyan, Vage Yunovich
core
KINETIC-PROPERTIES OF MAIZE GERM CYTOPLASMIC INORGANIC PYROPHOSPHATASE
An alkaline inorganic pyrophosphatase (pyrophosphate hydrolase EC 3.6. 1. 1.) was purified from maize (Zea mays) germ. This enzyme showed high specificty for inorganic pyrophosphate (PPi) with a K(m) value of 4.7 x 10(-5) M. The pH optimum was 8.0 to 8.5,
AOYAMA, H, BRANDAO, MEG
core
Inositol pyrophosphate 1,5-IP8 regulates expression of a fission yeast phosphate homeostasis regulon, comprising phosphate acquisition genes pho1, pho84, and tgp1, via its action as an agonist of precocious termination of transcription of the upstream ...
Shreya Ghosh +6 more
doaj +1 more source

