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Molecular Cloning and Characterization of a Soluble Inorganic Pyrophosphatase in Potato. [PDF]

Plant Physiology, 1995
A cDNA clone encoding a soluble inorganic pyrophosphatase (EC 3.6.1.1) of potato (Solanum tuberosum L.) was isolated by screening a developing tuber library with a heterologous probe. The central domain of the encoded polypeptide is nearly identical at the sequence level with its Arabidopsis homolog (J.J. Kieber and E.R. Signer [1991] Plant Mol Biol 16:
Christiane Gebhardt, Patrick Du Jardin, Robert Brasseur   +2 more
exaly   +4 more sources

Structure of the Mycobacterium tuberculosis Soluble Inorganic Pyrophosphatase Rv3628 at pH 7.0 [PDF]

Acta Crystallographica Section F: Structural Biology Communications, 2011
The 1.5 Å resolution crystal structure of the Mycobacterium tuberculosis soluble inorganic pyrophosphatase Rv3628 at pH 7.0 is reported. The M. tuberculosis and M. leprae genomes include genes for the only two family I inorganic pyrophosphatases known to contain two histidines in the active site. The role of these two residues in catalysis is not fully
Stefano Benini, Keith S Wilson
exaly   +5 more sources

Bacillus subtilis ORF yybQ Encodes a Manganese-Dependent Inorganic Pyrophosphatase With Distinctive Properties: The First of a New Class of Soluble Pyrophosphatase? [PDF]

Microbiology (United Kingdom), 1998
The N-terminal 15 amino acids of the major protein associated with inorganic pyrophosphatase activity in Bacillus subtilis WB600 are identical to those of B. subtilis ORF yybQ. This ORF was amplified from B. subtilis WB600 DNA by PCR and cloned into an overexpression vector in Escherichia coli.
Simon Ward, Nicholas J Kuhn
exaly   +3 more sources

Evolutionary Conservation of the Active Site of Soluble Inorganic Pyrophosphatase

Trends in Biochemical Sciences, 1992
Soluble inorganic pyrophosphatases (PPases) are essential enzymes that are important for controlling the cellular levels of inorganic pyrophosphate (PPi). Although prokaryotic and eukaryotic PPases differ substantially in amino acid sequence, recent evidence now demonstrates clearly that PPases throughout evolution show a remarkable level of ...
Alexander Baykov, Reijo Lahti
exaly   +3 more sources

The R78K and D117E Active-Site Variants of Saccharomyces cerevisiae Soluble Inorganic Pyrophosphatase: Structural Studies and Mechanistic Implications

Journal of Molecular Biology, 1998
We have solved the structure of two active-site variants of soluble inorganic pyrophosphatases (PPase), R78K and D117K, at resolutions of 1.85 and 2.15 A and R-factors of 19.5% and 18.3%, respectively. In the R78K variant structure, the high-affinity phosphate group (P1) is missing, consistent with the wild-type structure showing a bidentate ...
Barry S Cooperman, Adrian Goldman, Tommi Kajander   +2 more
exaly   +3 more sources

H⁺-Translocating Membrane-Bound Pyrophosphatase from Rhodospirillum rubrum Fuels Escherichia coli Cells via an Alternative Pathway for Energy Generation

Microorganisms, 2023
Inorganic pyrophosphatases (PPases) catalyze an essential reaction, namely, the hydrolysis of PPi, which is formed in large quantities as a side product of numerous cellular reactions.
Evgeniya A. Malykh, Liubov I. Golubeva, Ekaterina S. Kovaleva, Mikhail S. Shupletsov, Elena V. Rodina, Sergey V. Mashko, Nataliya V. Stoynova   +6 more
doaj   +1 more source

PPA1, an energy metabolism initiator, plays an important role in the progression of malignant tumors

Frontiers in Oncology, 2022
Inorganic pyrophosphatase (PPA1) encoded by PPA1 gene belongs to Soluble Pyrophosphatases (PPase) family and is expressed widely in various tissues of Homo sapiens, as well as significantly in a variety of malignancies.
Shuying Wang, Shuying Wang, Jianmei Wei, Shunwei Li, Yuyin Luo, Yuyin Luo, Yifei Li, Xianglin Wang, Xianglin Wang, Wenzhi Shen, Dehong Luo, Daishun Liu   +11 more
doaj   +1 more source

Biological properties and roles of a Trichinella spiralis inorganic pyrophosphatase in molting and developmental process of intestinal larval stages

Veterinary Research, 2021
Inorganic pyrophosphatase (PPase) participates in energy cycle and plays a vital role in hydrolysis of inorganic pyrophosphate (PPi) into inorganic phosphate (Pi).
Chen Xi Hu, Jie Zeng, Hui Nan Hao, Yang Xiu Yue Xu, Fang Liu, Ruo Dan Liu, Shao Rong Long, Zhong Quan Wang, Jing Cui   +8 more
doaj   +1 more source

Tissue-targeted inorganic pyrophosphate hydrolysis in a fugu5 mutant reveals that excess inorganic pyrophosphate triggers developmental defects in a cell-autonomous manner

Frontiers in Plant Science, 2022
Excess PPi triggers developmental defects in a cell-autonomous manner. The level of inorganic pyrophosphate (PPi) must be tightly regulated in all kingdoms for the proper execution of cellular functions. In plants, the vacuolar proton pyrophosphatase (H+-
Shizuka Gunji, Shizuka Gunji, Kensuke Kawade, Kensuke Kawade, Kensuke Kawade, Hiromitsu Tabeta, Hiromitsu Tabeta, Gorou Horiguchi, Gorou Horiguchi, Akira Oikawa, Akira Oikawa, Mariko Asaoka, Masami Yokota Hirai, Masami Yokota Hirai, Hirokazu Tsukaya, Ali Ferjani   +15 more
doaj   +1 more source

The structure of E.coli soluble inorganic pyrophosphatase at 2.7 Å resolution [PDF]

"Protein Engineering, Design and Selection", 1994
The structure of E.coli soluble inorganic pyrophosphatase has been refined at 2.7 A resolution to an R-factor of 20.9%. The overall fold of the molecule is essentially the same as yeast pyrophosphatase, except that yeast pyrophosphatase is longer at both the N- and C-termini.
J, Kankare, G S, Neal, T, Salminen, T, Glumoff, T ], Glumhoff T [corrected to Glumoff, B S, Cooperman, R, Lahti, A, Goldman   +7 more
openaire   +2 more sources