Results 31 to 40 of about 819,741 (161)

A Site‐Directed Mutagenesis Study of Saccharomyces cerevisiae Pyrophosphatase [PDF]

open access: yesEuropean Journal of Biochemistry, 1996
We report the expression and initial characterization of 19 active‐site variants of Saccharomyces cerevisiae inorganic pyrophosphatase (PPase), including measurements of thermostability, oligomeric structure and specific activity at pH 7.2. 13 of the 19 conservative substitutions resulted in at least a fivefold decrease in activity, indicating that ...
P, Heikinheimo   +7 more
openaire   +2 more sources

Hydrolysis of nucleoside di- and triphosphates by crystalline preparations of yeast inorganic pyrophosphatase [PDF]

open access: yes, 1960
1. 1. Crystalline preparations of yeast inorganic pyrophosphatase catalyze phosphate liberation from ATP and ADP, as well as from pyrophosphate, in the presence of zinc ions.2. 2.
Schlesinger, Milton J., Coon, Minor J.
core   +1 more source

Overexpression of the EuSIP5 Gene to Improve Drought Resistance in Tobacco

open access: yesHorticulturae
Soluble inorganic pyrophosphatase (s-PPase), a pyrophosphate hydrolase, is crucial for various physiological processes including plant growth and development, metabolic functions, and responses to abiotic stresses.
Yueling Lin   +3 more
doaj   +1 more source

New crystal forms ofEscherichia coliandSaccharomyces cerevisiaesoluble inorganic pyrophosphatases

open access: yesActa Crystallographica Section D Biological Crystallography, 1995
We have obtained new crystal forms of Escherichia coli and Saccharomyces cerevisiae soluble inorganic pyrophosphatase with and without substrate, competitive inhibitor and divalent cation. They diffract to higher resolution than any forms previously reported. The best E.
HEIKINHEIMO, P   +4 more
openaire   +3 more sources

Alkaline inorganic pyrophosphatase from immature wheat grains

open access: yes, 1983
Changes in the level of alkaline inorganic pyrophosphatase and ADPG-pyrophosphorylase were monitored in developing wheat grains at weekly intervals ...
R. Kumar, Singh, R., R. Singh, Kumar, R.
core   +1 more source

Differential Regulation of Soluble and Membrane-Bound Inorganic Pyrophosphatases in the Photosynthetic Bacterium Rhodospirillum rubrum Provides Insights into Pyrophosphate-Based Stress Bioenergetics [PDF]

open access: yesJournal of Bacteriology, 2004
ABSTRACT Soluble and membrane-bound inorganic pyrophosphatases (sPPase and H + -PPase, respectively) of the purple nonsulfur bacterium Rhodospirillum rubrum are differentially regulated by environmental growth conditions.
López-Marqués, Rosa L.   +3 more
openaire   +5 more sources

Properties and Distribution of Inorganic Pyrophosphatase in Rabbit Dental Pulp

open access: yes, 1977
Some properties and the intracellular distribution of inorganic pyrophosphatase in rabbit dental pulp were determined. This enzyme was sensitive to Mg2+, and not inhibited by imidazol and CN- which are inhibitors of alkaline phosphatase.
Masae Mitsuma   +4 more
core   +1 more source

Virus-Induced Gene Silencing of Plastidial Soluble Inorganic Pyrophosphatase Impairs Essential Leaf Anabolic Pathways and Reduces Drought Stress Tolerance inNicotiana benthamiana [PDF]

open access: yesPlant Physiology, 2010
AbstractThe role of pyrophosphate in primary metabolism is poorly understood. Here, we report on the transient down-regulation of plastid-targeted soluble inorganic pyrophosphatase in Nicotiana benthamiana source leaves. Physiological and metabolic perturbations were particularly evident in chloroplastic central metabolism, which is reliant on fast and
George G.M.   +6 more
openaire   +4 more sources

Alkaline inorganic pyrophosphatase from guar (Cyamopsis tetragonoloba) cotyledons

open access: yes, 1977
Alkaline inorganic pyrophosphatase from guar cotyledons was purified x 110 with about 34% recovery by (NH4)2SO4 fractionation, acetone ...
Singh, Randhir   +3 more
core   +1 more source

Functional characterization of EuSIP1 reveals its central role in trans-polyisoprene biosynthesis in Eucommia ulmoides

open access: yesIndustrial Crops and Products
Eucommia ulmoides is a unique plant capable of synthesizing trans-polyisoprene (TPI), a form of natural rubber with outstanding mechanical and chemical properties. In addition to its industrial importance, the bark of E.
Rui Cao   +8 more
doaj   +1 more source

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