A Site‐Directed Mutagenesis Study of Saccharomyces cerevisiae Pyrophosphatase [PDF]
We report the expression and initial characterization of 19 active‐site variants of Saccharomyces cerevisiae inorganic pyrophosphatase (PPase), including measurements of thermostability, oligomeric structure and specific activity at pH 7.2. 13 of the 19 conservative substitutions resulted in at least a fivefold decrease in activity, indicating that ...
P, Heikinheimo +7 more
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Hydrolysis of nucleoside di- and triphosphates by crystalline preparations of yeast inorganic pyrophosphatase [PDF]
1. 1. Crystalline preparations of yeast inorganic pyrophosphatase catalyze phosphate liberation from ATP and ADP, as well as from pyrophosphate, in the presence of zinc ions.2. 2.
Schlesinger, Milton J., Coon, Minor J.
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Overexpression of the EuSIP5 Gene to Improve Drought Resistance in Tobacco
Soluble inorganic pyrophosphatase (s-PPase), a pyrophosphate hydrolase, is crucial for various physiological processes including plant growth and development, metabolic functions, and responses to abiotic stresses.
Yueling Lin +3 more
doaj +1 more source
New crystal forms ofEscherichia coliandSaccharomyces cerevisiaesoluble inorganic pyrophosphatases
We have obtained new crystal forms of Escherichia coli and Saccharomyces cerevisiae soluble inorganic pyrophosphatase with and without substrate, competitive inhibitor and divalent cation. They diffract to higher resolution than any forms previously reported. The best E.
HEIKINHEIMO, P +4 more
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Alkaline inorganic pyrophosphatase from immature wheat grains
Changes in the level of alkaline inorganic pyrophosphatase and ADPG-pyrophosphorylase were monitored in developing wheat grains at weekly intervals ...
R. Kumar, Singh, R., R. Singh, Kumar, R.
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Differential Regulation of Soluble and Membrane-Bound Inorganic Pyrophosphatases in the Photosynthetic Bacterium Rhodospirillum rubrum Provides Insights into Pyrophosphate-Based Stress Bioenergetics [PDF]
ABSTRACT Soluble and membrane-bound inorganic pyrophosphatases (sPPase and H + -PPase, respectively) of the purple nonsulfur bacterium Rhodospirillum rubrum are differentially regulated by environmental growth conditions.
López-Marqués, Rosa L. +3 more
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Properties and Distribution of Inorganic Pyrophosphatase in Rabbit Dental Pulp
Some properties and the intracellular distribution of inorganic pyrophosphatase in rabbit dental pulp were determined. This enzyme was sensitive to Mg2+, and not inhibited by imidazol and CN- which are inhibitors of alkaline phosphatase.
Masae Mitsuma +4 more
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Virus-Induced Gene Silencing of Plastidial Soluble Inorganic Pyrophosphatase Impairs Essential Leaf Anabolic Pathways and Reduces Drought Stress Tolerance inNicotiana benthamiana [PDF]
AbstractThe role of pyrophosphate in primary metabolism is poorly understood. Here, we report on the transient down-regulation of plastid-targeted soluble inorganic pyrophosphatase in Nicotiana benthamiana source leaves. Physiological and metabolic perturbations were particularly evident in chloroplastic central metabolism, which is reliant on fast and
George G.M. +6 more
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Alkaline inorganic pyrophosphatase from guar (Cyamopsis tetragonoloba) cotyledons
Alkaline inorganic pyrophosphatase from guar cotyledons was purified x 110 with about 34% recovery by (NH4)2SO4 fractionation, acetone ...
Singh, Randhir +3 more
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Eucommia ulmoides is a unique plant capable of synthesizing trans-polyisoprene (TPI), a form of natural rubber with outstanding mechanical and chemical properties. In addition to its industrial importance, the bark of E.
Rui Cao +8 more
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