Tandem Inactivation of Inositol Pyrophosphatases Asp1, Siw14, and Aps1 Illuminates Functional Redundancies in Inositol Pyrophosphate Catabolism in Fission Yeast [PDF]
MBioInositol pyrophosphates 5-IP7, 1-IP7, and 1,5-IP8 are eukaryal signaling molecules that influence cell physiology, especially phosphate homeostasis. In fission yeast, 1,5-IP8 and 1-IP7 impact gene expression by acting as agonists of RNA 3'-processing and
Stewart Shuman, Henning J Jessen
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Conformational dynamics and asymmetry in multimodal inhibition of membrane-bound pyrophosphatases [PDF]
eLifeMembrane-bound pyrophosphatases (mPPases) are homodimeric proteins that hydrolyse pyrophosphate and pump H+/Na+ across membranes. They are crucial for the virulence of protist pathogens, making them attractive drug targets.
Jianing Liu +15 more
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Functional and Structural Asymmetry Suggest a Unifying Principle for Catalysis in Membrane-Bound Pyrophosphatases [PDF]
EMBO ReportsMembrane-bound pyrophosphatases (M-PPases) are homodimeric primary ion pumps that couple the transport of Na+- and/or H+ across membranes to the hydrolysis of pyrophosphate.
Jannik Strauss +2 more
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Escherichia coli DnaE Polymerase Couples Pyrophosphatase Activity to DNA Replication. [PDF]
PLoS ONE, 2016 DNA Polymerases generate pyrophosphate every time they catalyze a step of DNA elongation. This elongation reaction is generally believed as thermodynamically favoured by the hydrolysis of pyrophosphate, catalyzed by inorganic pyrophosphatases.
Fabio Lapenta +7 more
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Pyrophosphate homeostasis in multiple subcellular compartments is essential in Plasmodium falciparum [PDF]
mBioPyrophosphate is a byproduct of numerous cellular reactions that use ATP or other nucleoside triphosphates to synthesize DNA, RNA, protein, and various molecules.
Ikechukwu Nwankwo, Hangjun Ke
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μ₃-Oxo nucleophile formation enables efficient S N 2 hydrolysis at the trinuclear metal center in inorganic pyrophosphatase [PDF]
Communications ChemistryInorganic pyrophosphatases are essential metalloenzymes for phosphate metabolism. Bacterial Family II Inorganic pyrophosphatases utilize a trinuclear metal center and exhibit higher catalytic activity than binuclear counterparts.
Saki Maruoka +10 more
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Activities and genetic interactions of fission yeast Aps1, a Nudix-type inositol pyrophosphatase and inorganic polyphosphatase [PDF]
mBioInositol pyrophosphate 1,5-IP8 regulates expression of a fission yeast phosphate homeostasis regulon, comprising phosphate acquisition genes pho1, pho84, and tgp1, via its action as an agonist of precocious termination of transcription of the upstream ...
Shreya Ghosh +6 more
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Biomolecules, 2020 Quaternary structure of CBS-pyrophosphatases (CBS-PPases), which belong to the PPases of family II, plays an important role in their function ensuring cooperative behavior of the enzymes.
Dmitri Svergun +2 more
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Conformational Dynamics of Mitochondrial Inorganic Pyrophosphatase hPPA2 and Its Changes Caused by Pathogenic Mutations [PDF]
LifeInorganic pyrophosphatases, or PPases, are ubiquitous enzymes whose activity is necessary for a large number of biosynthetic reactions. The catalytic function of PPases is dependent on certain conformational changes that have been previously ...
Ekaterina Bezpalaya +3 more
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