Results 181 to 190 of about 66,823 (327)

Intracellular protein crystallization in living insect cells

FEBS Open Bio, Volume 15, Issue 4, Page 551-562, April 2025.
This research protocol provides a step‐by‐step guide for applying the InCellCryst pipeline for intracellular protein crystallization. After gene cloning and generation of recombinant baculoviruses, High Five insect cells are infected for target protein crystallization.
Robert Schönherr, Nina Eichler, Fatama A. Sornaly, Juliane Boger, Anne M. Frevert, Janine Mia Lahey‐Rudolph, Hannah Meyer, Lisa Weymar, Lars Redecke   +8 more
wiley   +1 more source

Application of Liquid Chromatography Coupled to Mass Spectrometry for Direct Estimation of the Total Levels of Adenosine and Its Catabolites in Human Blood

Pharmaceuticals
Adenosine is a multifunctional nucleoside with several roles across various levels in organisms. Beyond its intracellular involvement in cellular metabolism, extracellular adenosine potently influences both physiological and pathological processes.
Jakub Šofranko, Peter Mitro, Zora Lazúrová, Martin Jozef Péč, Tomáš Bolek, Renata Péčová, Matúš Dohál, Matej Samoš, Radovan Murín   +8 more
doaj   +1 more source

Changes in Coronary Venous Inosine Concentration and Myocardial Wall Thickening during Regional Ischemia in the Pig [PDF]

, 1974
Jan Willem de Jong, Sidney Goldstein
openalex   +1 more source

Adenosine Monophosphate Improves Lipolysis in Obese Mice by Reducing DNA Methylation via ADORA2A Activation by Ecto‐5′‐Nucleotidase (CD73)

Advanced Science, Volume 12, Issue 14, April 10, 2025.
In this study, the improvement effect of AMP on obesity can be attributed to the conversion of extracellular AMP to ADO through ADORA2A activation by CD73, which can promote lipolysis by reducing DNA methylation level and enhancing HSL gene transcription. These findings show the great potential of AMP in improving obesity.
Zhijuan Cui, Li Feng, Sujuan Rao, Zihao Huang, Shuangbo Huang, Liudan Liu, Yuan Liao, Zheng Lan, Qiling Chen, Jinping Deng, Leli Wang, Yulong Yin, Chengquan Tan   +12 more
wiley   +1 more source

An Untargeted Metabolomic Analysis of Lacticaseibacillus (L.) rhamnosus, Lactobacillus (L.) acidophilus, Lactiplantibacillus (L.) plantarum and Limosilactobacillus (L.) reuteri Reveals an Upregulated Production of Inosine from L. rhamnosus

Microorganisms
Lactic acid bacteria are considered an inexhaustible source of bioactive compounds; indeed, products from their metabolism are known to have immunomodulatory and anti-inflammatory activity.
Luca Spaggiari, Natalia Pedretti, Francesco Ricchi, Diego Pinetti, Giuseppina Campisciano, Francesco De Seta, Manola Comar, Samyr Kenno, Andrea Ardizzoni, Eva Pericolini   +9 more
doaj   +1 more source

The effect of purine and pyrimidine derivatives on a spectrophotometric assay of enolase [PDF]

, 1963
Thesis (M.A.)--Boston UniversityIn studies on the formation of 2,3-diphosphoglycerate (2,3-DPG) from inosine by erythrocyte preparations it was observed that the inosine interfered with the enzymatic assay for 2,3-DPG. Phosphoglyceromutase, for which 2,3-
McLaughlin, Mary E. Heron
core  

Synthetic RNA and DNA Duplexes. Premelting, Melting and Postmelting Transitions of Alternating Inosine-Cytosine Polynucleotides in Solution [PDF]

, 1978
Dinshaw J. Patel
openalex   +1 more source

Developing glycosylase‐based T‐to‐G and C‐to‐K base editors in rice

Plant Biotechnology Journal, EarlyView.
Yongjie Kuang, Xuemei Wu, Meijie Liu, Fang Yan, Dongfang Ma, Xueping Zhou, Huanbin Zhou, Bin Ren   +7 more
wiley   +1 more source

Advancing Precision Medicine: Recent Innovations in Gene Editing Technologies

Advanced Science, Volume 12, Issue 14, April 10, 2025.
This review article delves into the cutting‐edge advancements in gene editing technology, with a particular focus on prime editor proteins and their engineered variants. The authors provide a thorough examination of recent developments that have addressed key challenges in the field, including improved precision, enhanced editing efficiency, and ...
Abhijith Koodamvetty, Saravanabhavan Thangavel   +1 more
wiley   +1 more source

A Comparison on the Coordination Tendency towards Cu²⁺ of the Base Moieties in Guanosine, Inosine and Adenosine 5′‐Triphosphates [PDF]

, 1974
Helmut Sigel, Christoph F. Naumann, Bernhard PRUS   +2 more
openalex   +1 more source