Results 31 to 40 of about 45,101 (293)

Development of an improved Pseudoalteromonas haloplanktis TAC125 strain for recombinant protein secretion at low temperature [PDF]

, 2008
Background: In a previous paper, we reported the accomplishment of a cold gene-expression system for the recombinant secretion of heterologous proteins in Pseudoalteromonas haloplanktis TAC125.
CIRULLI C., DE VIZIO D., PARRILLI, ERMENEGILDA, TUTINO, MARIA LUISA   +3 more
core   +1 more source

Chromosomal DNA deletion confers phage resistance to Pseudomonas aeruginosa. [PDF]

, 2014
Bacteria develop a broad range of phage resistance mechanisms, such as prevention of phage adsorption and CRISPR/Cas system, to survive phage predation.
He, Xuesong, Hu, Fuquan, Jin, Xiaolin, Le, Shuai, Li, Ming, Lu, Shuguang, Lux, Renate, Rao, Xiancai, Shi, Wenyuan, Tan, Yinling, Wang, Jing, Wu, Nicholas C, Yao, Xinyue, Zhao, Yan   +13 more
core   +1 more source

Insertion of the LINE Retrotransposon MGL Causes a Conidiophore Pattern Mutation in Magnaporthe grisea

Molecular Plant-Microbe Interactions, 2000
We obtained three Magnaporthe grisea morphological mutants that had the LINE transposon MGL inserted into the ACR1 locus. Sequence analysis revealed that ACR1 is homologous to medA, a developmental regulator of Aspergillus nidulans conidiation.
Marie Nishimura, Nagao Hayashi, Nam-Soo Jwa, Gee W. Lau, John E. Hamer, Akira Hasebe   +5 more
doaj   +1 more source

Safe engineering of CAR T cells for adoptive cell therapy of cancer using long‐term episomal gene transfer

EMBO Molecular Medicine, 2016
Chimeric antigen receptor (CAR) T‐cell therapy is a new successful treatment for refractory B‐cell leukemia. Successful therapeutic outcome depends on long‐term expression of CAR transgene in T cells, which is achieved by delivering transgene using ...
Chuan Jin, Grammatiki Fotaki, Mohanraj Ramachandran, Berith Nilsson, Magnus Essand, Di Yu   +5 more
doaj   +1 more source

A robust protocol for efficient generation, and genomic characterization of insertional mutants of Chlamydomonas reinhardtii

Plant Methods, 2017
Background Random insertional mutagenesis of Chlamydomonas reinhardtii using drug resistance cassettes has contributed to the generation of tens of thousands of transformants in dozens of labs around the world. In many instances these insertional mutants
Steve V. Pollock, Bratati Mukherjee, Joanna Bajsa-Hirschel, Marylou C. Machingura, Ananya Mukherjee, Arthur R. Grossman, James V. Moroney   +6 more
doaj   +1 more source

TNF receptor–related factor 3 inactivation promotes the development of intrahepatic cholangiocarcinoma through NF‐κB‐inducing kinase–mediated hepatocyte transdifferentiation

Hepatology, EarlyView., 2022
Abstract Background and Aims Intrahepatic cholangiocarcinoma (ICC) is a deadly but poorly understood disease, and its treatment options are very limited. The aim of this study was to identify the molecular drivers of ICC and search for therapeutic targets.
Yuto Shiode, Takahiro Kodama, Satoshi Shigeno, Kazuhiro Murai, Satoshi Tanaka, Justin Y. Newberg, Jumpei Kondo, Shogo Kobayashi, Ryoko Yamada, Hayato Hikita, Ryotaro Sakamori, Hiroshi Suemizu, Tomohide Tatsumi, Hidetoshi Eguchi, Nancy A. Jenkins, Neal G. Copeland, Tetsuo Takehara   +16 more
wiley   +1 more source

HERVs, transposons and human diseases : part II [PDF]

, 2013
Part 1 and part 3 of the article can be found through this link : https://www.um.edu.mt/library/oar//handle/123456789/12961 https://www.um.edu.mt/library/oar//handle/123456789/13132Part 2 of the article.
Baldacchino, Sandra, Grech, Alfred
core   +2 more sources

Integration Mapping of piggyBac-Mediated CD19 Chimeric Antigen Receptor T Cells Analyzed by Novel Tagmentation-Assisted PCR

EBioMedicine, 2018
Insertional mutagenesis is an important risk with all genetically modified cell therapies, including chimeric antigen receptor (CAR)-T cell therapy used for hematological malignancies.
Motoharu Hamada, Nobuhiro Nishio, Yusuke Okuno, Satoshi Suzuki, Nozomu Kawashima, Hideki Muramatsu, Shoma Tsubota, Matthew H. Wilson, Daisuke Morita, Shinsuke Kataoka, Daisuke Ichikawa, Norihiro Murakami, Rieko Taniguchi, Kyogo Suzuki, Daiei Kojima, Yuko Sekiya, Eri Nishikawa, Atsushi Narita, Asahito Hama, Seiji Kojima, Yozo Nakazawa, Yoshiyuki Takahashi   +21 more
doaj   +1 more source

Application of transposon insertion site sequencing method in the exploration of gene function in microalgae

Frontiers in Microbiology, 2023
Microalgae are a large group of organisms that can produce various useful substances through photosynthesis. Microalgae need to be genetically modified at the molecular level to become “Chassis Cells” for food, medicine, energy, and environmental ...
Xiaobing Hu, Xiaobing Hu, Yulong Fan, Chengfeng Mao, Hui Chen, Qiang Wang, Qiang Wang   +6 more
doaj   +1 more source

A Review of Genetic Transfer Therapy Trials from a Regulatory Perspective for the Scientific Understanding [PDF]

, 2013
The present work reviews the safety and efficacy concerns of the Recombinant DNA Advisory Committee (RAC) regarding Gene Transfer Therapy (GTT) clinical trials.
Olguin, Arturo
core   +2 more sources