Results 51 to 60 of about 199,615 (291)

Preanalytical Strategies for Native Mass Spectrometry Analysis of Protein Modifications, Complexes, and Higher-Order Structures

AppliedChem
Proteins are essential biological macromolecules that play key regulatory roles in all biological processes. Abnormalities in these processes are often reflected in proteins, manifesting as changes in their structure, sequence, folding state ...
Navid J. Ayon
doaj   +1 more source

Glycosylation States on Intact Proteins Determined by NMR Spectroscopy

Molecules, 2021
Protein glycosylation is important in many organisms for proper protein folding, signaling, cell adhesion, protein-protein interactions, and immune responses.
Audra A. Hargett, Aaron M. Marcella, Huifeng Yu, Chao Li, Jared Orwenyo, Marcos D. Battistel, Lai-Xi Wang, Darón I. Freedberg   +7 more
doaj   +1 more source

Proteome Profiling of Breast Tumors by Gel Electrophoresis and Nanoscale Electrospray Ionization Mass Spectrometry [PDF]

, 2008
We have conducted proteome-wide analysis of fresh surgery specimens derived from breast cancer patients, using an approach that integrates size-based intact protein fractionation, nanoscale liquid separation of peptides, electrospray ion trap mass ...
Aebersold R., Boyle E. I., Chong P. K., Cottingham K., Cove D. H., Ezeh U. I., Foran E., Gygi S. P., Ioachim E., Jacobs J. M., Keller A., Laemmli U. K., Lapillonne A., Lin C. S., Liu H., Maclean B., Mathelin C., Metodiev M. V., old W. M., Patterson S. D., Pedrioli P. G., Rauch A., Schmidt A., Sealfon R. S., Seo J., Sheen-Cheen S. M., Silva J., Taylor M. R., Toi M., Wolters D. A., Zhang J.   +30 more
core   +1 more source

Quantification of Staphylococcal Enterotoxin A Variants at Low Level in Dairy Products by High-Resolution Top-Down Mass Spectrometry

Toxins
Food poisoning outbreaks frequently involve staphylococcal enterotoxins (SEs). SEs include 33 distinct types and multiple sequence variants per SE type.
Nina Aveilla, Cécile Feraudet-Tarisse, Dominique Marcé, Abdelhak Fatihi, François Fenaille, Jacques-Antoine Hennekinne, Stéphanie Simon, Yacine Nia, François Becher   +8 more
doaj   +1 more source

Analysis of Metals in Whole Cells, Thylakoids and Photosynthetic Protein Complexes in Synechocystis sp. PCC6803

Bio-Protocol, 2018
Metals are essential in many biological processes, including oxygenic photosynthesis. Here we described a method to measure the metal pool in whole cells and thylakoids, including the bioactive pool in intact photosynthetic protein complexes in the model
Chiara Gandini, Søren Husted, Sidsel Schmidt   +2 more
doaj   +1 more source

Proteomic screen reveals diverse protein transport between connected neurons in the visual system

Cell Reports, 2022
Summary: Intercellular transfer of toxic proteins between neurons is thought to contribute to neurodegenerative disease, but whether direct interneuronal protein transfer occurs in the healthy brain is not clear.
Lucio M. Schiapparelli, Pranav Sharma, Hai-Yan He, Jianli Li, Sahil H. Shah, Daniel B. McClatchy, Yuanhui Ma, Han-Hsuan Liu, Jeffrey L. Goldberg, John R. Yates, III, Hollis T. Cline   +10 more
doaj   +1 more source

The role of the mobile proton in fucose migration [PDF]

, 2019
Fucose migration reactions represent a substantial challenge in the analysis of fucosylated glycan structures by mass spectrometry. In addition to the well-established observation of transposed fucose residues in glycan-dissociation product ions, recent ...
Helden, Gert von, Lettow, Maike, Manz, Christian, Marianski, Mateusz, Meijer, Gerard, Mucha, Eike, Pagel, Kevin, Thomas, Daniel A.   +7 more
core   +1 more source

Expression, Purification and Characterization of Ricin vectors used for exogenous antigen delivery into the MHC Class I presentation pathway

Biological Procedures Online, 2003
Disarmed versions of the cytotoxin ricin can deliver fused peptides into target cells leading to MHC class I-restricted antigen presentation [Smith et al. J Immunol 2002; 169:99-107].
Smith Daniel C., Marsden Catherine J., Lord J. Michael, Roberts Lynne M.   +3 more
doaj   +1 more source

Multi-lectin Affinity Chromatography and Quantitative Proteomic Analysis Reveal Differential Glycoform Levels between Prostate Cancer and Benign Prostatic Hyperplasia Sera. [PDF]

, 2018
Currently prostate-specific antigen is used for prostate cancer (PCa) screening, however it lacks the necessary specificity for differentiating PCa from other diseases of the prostate such as benign prostatic hyperplasia (BPH), presenting a clinical need
Adusumilli, Ravali, Brooks, James D, Kullolli, Majlinda, Mallick, Parag, Pitteri, Sharon J, Tanimoto, Cheylene, Totten, Sarah M   +6 more
core   +2 more sources

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source