Results 31 to 40 of about 106,020 (256)

In situ analysis of intact proteins by ion mobility mass spectrometry [PDF]

TrAC Trends in Analytical Chemistry, 2020
Abstract We describe ion mobility mass spectrometry techniques for the in situ analysis of intact proteins, i.e., the analysis of proteins directly from their biological environment. The benefits of in situ analysis include those associated more broadly with analysis of intact proteins, e.g., retention of connectivity between post-translational ...
Emma K. Sisley, Eva Illes-Toth, Helen J. Cooper   +2 more
openaire   +1 more source

Two-dimensional liquid chromatography protein expression mapping for differential proteomic analysis of normal and O157:H7 Escherichia coli

BioTechniques, 2003
A multidimensional chromatographic method has been applied for the differential analysis of proteins from different strains of Escherichia coli bacteria.
Suping Zheng, Kimberly A. Schneider, Timothy J. Barder, David M. Lubman   +3 more
doaj   +1 more source

Intact protein barcoding enables one-shot identification of CRISPRi strains and their metabolic state

Cell Reports: Methods
Summary: Detecting strain-specific barcodes with mass spectrometry can facilitate the screening of genetically engineered bacterial libraries. Here, we introduce intact protein barcoding, a method to measure protein-based library barcodes and metabolites
Vanessa Pahl, Paul Lubrano, Felicia Troßmann, Daniel Petras, Hannes Link   +4 more
doaj   +1 more source

Preanalytical Strategies for Native Mass Spectrometry Analysis of Protein Modifications, Complexes, and Higher-Order Structures

AppliedChem
Proteins are essential biological macromolecules that play key regulatory roles in all biological processes. Abnormalities in these processes are often reflected in proteins, manifesting as changes in their structure, sequence, folding state ...
Navid J. Ayon
doaj   +1 more source

Analysis of Metals in Whole Cells, Thylakoids and Photosynthetic Protein Complexes in Synechocystis sp. PCC6803

Bio-Protocol, 2018
Metals are essential in many biological processes, including oxygenic photosynthesis. Here we described a method to measure the metal pool in whole cells and thylakoids, including the bioactive pool in intact photosynthetic protein complexes in the model
Chiara Gandini, Søren Husted, Sidsel Schmidt   +2 more
doaj   +1 more source

Mass spectrometry for the evaluation of monoclonal proteins in multiple myeloma and related disorders: an International Myeloma Working Group Mass Spectrometry Committee Report

Blood Cancer Journal, 2021
Plasma cell disorders (PCDs) are identified in the clinical lab by detecting the monoclonal immunoglobulin (M-protein) which they produce. Traditionally, serum protein electrophoresis methods have been utilized to detect and isotype M-proteins ...
David L. Murray, Noemi Puig, Sigurdur Kristinsson, Saad Z. Usmani, Angela Dispenzieri, Giada Bianchi, Shaji Kumar, Wee Joo Chng, Roman Hajek, Bruno Paiva, Anders Waage, S. Vincent Rajkumar, Brian Durie   +12 more
doaj   +1 more source

Quantification of Staphylococcal Enterotoxin A Variants at Low Level in Dairy Products by High-Resolution Top-Down Mass Spectrometry

Toxins
Food poisoning outbreaks frequently involve staphylococcal enterotoxins (SEs). SEs include 33 distinct types and multiple sequence variants per SE type.
Nina Aveilla, Cécile Feraudet-Tarisse, Dominique Marcé, Abdelhak Fatihi, François Fenaille, Jacques-Antoine Hennekinne, Stéphanie Simon, Yacine Nia, François Becher   +8 more
doaj   +1 more source

Glycosylation States on Intact Proteins Determined by NMR Spectroscopy

Molecules, 2021
Protein glycosylation is important in many organisms for proper protein folding, signaling, cell adhesion, protein-protein interactions, and immune responses.
Audra A. Hargett, Aaron M. Marcella, Huifeng Yu, Chao Li, Jared Orwenyo, Marcos D. Battistel, Lai-Xi Wang, Darón I. Freedberg   +7 more
doaj   +1 more source

Sequencing intact membrane proteins using MALDI mass spectrometry

Frontiers in Analytical Science, 2023
Membrane proteins are key players in many cellular events and represent crucial drug targets. Matrix-assisted laser desorption ionization mass spectrometry (MALDI MS) is a valuable approach to investigate them. To our knowledge, there are only a few reports of sequencing small membrane proteins using MALDI in-source decay (ISD).
Zhamungui Sánchez, Edison, Hijazi, Hassan, Haidar, Jana, Mecarelli, Enrica, Bauda, Elda, Petit-Härtlein, Isabelle, Teulon, Jean-Marie, Pellequer, Jean-Luc, Boeri Erba, Elisabetta   +8 more
openaire   +3 more sources

Proteomic screen reveals diverse protein transport between connected neurons in the visual system

Cell Reports, 2022
Summary: Intercellular transfer of toxic proteins between neurons is thought to contribute to neurodegenerative disease, but whether direct interneuronal protein transfer occurs in the healthy brain is not clear.
Lucio M. Schiapparelli, Pranav Sharma, Hai-Yan He, Jianli Li, Sahil H. Shah, Daniel B. McClatchy, Yuanhui Ma, Han-Hsuan Liu, Jeffrey L. Goldberg, John R. Yates, III, Hollis T. Cline   +10 more
doaj   +1 more source