Results 41 to 50 of about 106,020 (256)

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Expression, Purification and Characterization of Ricin vectors used for exogenous antigen delivery into the MHC Class I presentation pathway

Biological Procedures Online, 2003
Disarmed versions of the cytotoxin ricin can deliver fused peptides into target cells leading to MHC class I-restricted antigen presentation [Smith et al. J Immunol 2002; 169:99-107].
Smith Daniel C., Marsden Catherine J., Lord J. Michael, Roberts Lynne M.   +3 more
doaj   +1 more source

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

FEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Kenji Sugase   +3 more
wiley   +1 more source

Identification and Interrogation of Combinatorial Histone Modifications

Frontiers in Genetics, 2013
Histone proteins are dynamically modified to mediate a variety of cellular processes including gene transcription, DNA damage repair, and apoptosis. Regulation of these processes occurs through the recruitment of non-histone proteins to chromatin by ...
Kelly R Karch, Jamie E DeNizio, Ben E Black, Benjamin Aaron Garcia   +3 more
doaj   +1 more source

Quantitative Mass Spectrometry Imaging of Intact Proteins in Tissues

Analytical Chemistry
Quantitative imaging of endogenous proteins in biological tissues is essential for understanding their roles in cellular signaling and metabolism. Mass spectrometry imaging (MSI) using nanospray desorption electrospray ionization (nano-DESI) is a label-free approach for mapping intact proteins in biological tissues with minimal sample preparation ...
Mushfeqa Iqfath, Andrew K. Swansiger, Manxi Yang, Lily Miller, Alyssa M. Moore, Emerson Hernly, Yunpeng Bai, Zhong-Yin Zhang, James S. Prell, Julia Laskin   +9 more
openaire   +2 more sources

Modulation of Homer1 EVH1 domain internal dynamics by putative autism‐associated mutations

FEBS Letters, EarlyView.
The putative autism‐associated M65I and S97L variants of the EVH1 domain of the postsynaptic scaffold protein Homer1 do not exhibit substantial changes in their overall structure or partner binding. Both of them, but especially the M65I variant, show altered internal dynamics relative to the wild‐type domain on the μs‐ms timescale, indicated by the ...
Fanni Farkas, Brigitta Maruzs, Zsófia E. Kálmán, Tomas Klumpler, Gyula Batta, Bálint Péterfia, Zoltán Gáspári   +6 more
wiley   +1 more source

Acoustic ejection mass spectrometry: An integrated pipeline for ultra-high throughput screening, reactivity profiling, and potency analysis of covalent BTK inhibitors

SLAS Technology
Covalent drug discovery has garnered renewed interest due to its potential to target proteins previously considered ''undruggable.'' Intact protein mass spectrometry (MS) is a critical technique for providing direct evidence of covalent drug ...
Ronghai Cheng, Adil Muneer, Maria Hercher, Bekim Bajrami, Reza Nemati   +4 more
doaj   +1 more source

Advances in the Mass Spectrometry of Membrane Proteins: From Individual Proteins to Intact Complexes

Annual Review of Biochemistry, 2011
Rapid advances in structural genomics and in large-scale proteomic projects have yielded vast amounts of data on soluble proteins and their complexes. Despite these advances, progress in studying membrane proteins using mass spectrometry (MS) has been slow.
Barrera, Nelson P., Robinson, Carol V.
openaire   +6 more sources

Separation methods hyphenated to mass spectrometry for the characterization of the protein glycosylation at the intact level

Journal of Pharmaceutical and Biomedical Analysis, 2020
Glycosylation is one of the most common post-translational modifications of proteins that affects their biological activity, solubility, and half-life. Therefore, its characterization is of great interest in proteomic, particularly from a diagnostic and therapeutic point of view.
Camperi, Julien, Pichon, Valérie, Delaunay, Nathalie   +2 more
openaire   +3 more sources

Comprehensive LESA Mass Spectrometry Imaging of Intact Proteins by Integration of Cylindrical FAIMS [PDF]

Analytical Chemistry, 2020
The benefits of high field asymmetric waveform ion mobility spectrometry (FAIMS) for mass spectrometry imaging of intact proteins in thin tissue sections have been demonstrated previously. In those works, a planar FAIMS device coupled with a Thermo Elite mass spectrometer was employed. Here, we have evaluated a newly introduced cylindrical FAIMS device
Rian L. Griffiths, James W. Hughes, Susan E. Abbatiello, Michael W. Belford, Iain B. Styles, Helen J. Cooper   +5 more
openaire   +3 more sources