Results 21 to 30 of about 106,020 (256)
Protein complexes in cells by AI‐assisted structural proteomics
Molecular Systems Biology, 2023 Accurately modeling the structures of proteins and their complexes using artificial intelligence is revolutionizing molecular biology. Experimental data enable a candidate‐based approach to systematically model novel protein assemblies.
Francis J O'Reilly +9 more
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iScience, 2021 Summary: Standard methods for mass analysis measure ensembles of thousand to millions of molecules. This approach enables analysis of monodisperse recombinant proteins, whereas some heterogeneous protein assemblies pose a significant challenge, whereby ...
Szu-Hsueh Lai +2 more
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Intact protein mass spectrometry and top-down proteomics [PDF]
Expert Review of Proteomics, 2013 American Society for Mass Spectrometry Sanibel meeting on top-down mass spectrometry St Pete Beach, FL, USA, 24-27 January 2013 Top-down mass spectrometry involves analysis of intact proteins, typically using electrospray ionization, as multiple charging enhances dissociation and thus identification by comparison of precursor and product ion masses ...
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Frontiers in Molecular Biosciences, 2022 Monoclonal antibodies (mAbs) are widely used as analytical components in immunoassays to detect target molecules in applications such as clinical diagnostics, food analysis and drug discovery.
Luise Luckau +5 more
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Ambient surface mass spectrometry–ion mobility spectrometry of intact proteins
Current Opinion in Chemical Biology, 2018 Ambient surface mass spectrometry encompasses a broad range of sampling and ionization techniques. To date, only a small subset of these, based on liquid microjunction extraction, have proven suitable for intact protein analysis from thin tissue sections. Liquid extraction surface analysis shows particular promise for this application.
Rian L, Griffiths +2 more
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Charge Reduction Stabilizes Intact Membrane Protein Complexes for Mass Spectrometry [PDF]
Journal of the American Chemical Society, 2014 The study of intact soluble protein assemblies by means of mass spectrometry is providing invaluable contributions to structural biology and biochemistry. A recent breakthrough has enabled similar study of membrane protein complexes, following their release from detergent micelles in the gas phase.
Mehmood, Shahid +6 more
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Recent Progress in FD-LC-MS/MS Proteomics Method
Frontiers in Chemistry, 2021 Through the course of our bio-analytical chemistry studies, we developed a novel proteomics analysis method, FD-LC-MS/MS (fluorogenic derivatization-liquid chromatography-tandem mass spectrometry). This method consists of fluorogenic derivatization (FD),
Hiroshi Kobayashi +2 more
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Analyzing Intact Proteins with Mass Spectrometry [PDF]
Cancer Discovery, 2011 Abstract A new top-down method of identifying intact proteins is particularly suited to differentiating highly related protein species created by alternative splicing, proteolytic events, and post-translational modifications.
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PLoS ONE, 2018 Cyanobacteria represent a bacterial phyllum characteristic by the ability to photosynthesize. They are potentially applicable for the production of useful compounds but may also cause poisoning or at least health problems as they can produce cyanotoxins.
Marek Šebela +4 more
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Characterization of Stability and Small-scale Structural Differences of Intact Glycoprotein Complexes Using Native Mass Spectrometry Travelling-Wave Ion Mobility Spectrometry [PDF]
Zhipu Xuebao, 2022 Glycosylation is one of the most common types of post-translational modifications (PTMs) of proteins, which affects protein properties including conformation, stability and solubility, and plays essential roles in functions including molecular ...
XU Xiang1,2 +2 more
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