Top-Down and Intact Protein Mass Spectrometry Data Visualization for Proteoform Analysis Using VisioProt-MS [PDF]
Bioinformatics and Biology Insights, 2019 The rise of intact protein analysis by mass spectrometry (MS) was accompanied by an increasing need for flexible tools allowing data visualization and analysis.
Jean Lesne +3 more
doaj +3 more sources
Investigation of intact protein complexes by mass spectrometry [PDF]
Mass Spectrometry Reviews, 2004 Abstract I. Introduction 00 II. Electrospray Ionization of Biomacromolecules 00 III. Mass Analyzers for Biomacromolecular Mass Spectrometry 00 A. Collisional Cooling and/or Focusing 00 B. Alternative Mass Analyzers 00 IV. Solution‐Phase Properties of Proteins Complexes 00 A.
Albert Heck
exaly +4 more sources
Mass spectrometry of intact protein complexes [PDF]
Nature Methods, 2013 Mass spectrometry technology to detect and characterize large, intact protein complexes is becoming more accessible.
Doerr Allison, Allison Doerr
exaly +2 more sources
Detection of intact megaDalton protein assemblies of vanillyl‐alcohol oxidase by mass spectrometry [PDF]
Protein Science, 2000 AbstractWell‐resolved ion signals of intact large protein assemblies, with molecular masses extending above one million Dalton, have been detected and mass analyzed using electrospray ionization mass spectrometry, with an uncertainty in mass of <0.2%.
Albert Heck +2 more
exaly +5 more sources
Intact protein bioanalysis by liquid chromatography – High-resolution mass spectrometry
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 2019 This review discusses the challenges of quantitative protein bioanalysis by LC-MS at the protein level. We will notably address the possibilities and current limitations of protein sample preparation, separation by LC, the challenge of interpreting protein ESI-MS spectra and the options for protein quantification based on extracted ion chromatograms or
Oladapo Olaleye +2 more
exaly +4 more sources
Mass spectrometry of intact membrane protein complexes [PDF]
Nature Protocols, 2013 Mass spectrometry (MS) of intact soluble protein complexes has emerged as a powerful technique to study the stoichiometry, structure-function and dynamics of protein assemblies. Recent developments have extended this technique to the study of membrane protein complexes, where it has already revealed subunit stoichiometries and specific phospholipid ...
Laganowsky, A +3 more
openaire +4 more sources
Characterizing Intact IgA2 Antibody and Its Size Variants Using Native Mass Spectrometry and a Tandem MS-based Charge Reduction Approach [PDF]
Zhipu Xuebao, 2022 Characterization of protein structures at multiple levels plays an essential role in study on protein functions. Structural analysis at the intact protein or intact complex level allows access of information on aspects including level of post ...
CHEN Qing-rong;CHAIHU Ling-xiao;CHEN Tao;WANG Guan-bo
doaj +1 more source
Applications of Tandem Mass Spectrometry (MS/MS) in Protein Analysis for Biomedical Research
Molecules, 2022 Mass Spectrometry (MS) allows the analysis of proteins and peptides through a variety of methods, such as Electrospray Ionization-Mass Spectrometry (ESI-MS) or Matrix-Assisted Laser Desorption Ionization-Mass Spectrometry (MALDI-MS).
Anca-Narcisa Neagu +5 more
doaj +1 more source
Analysis of Intact Protein Isoforms by Mass Spectrometry [PDF]
Journal of Biological Chemistry, 2011 The diverse proteome of an organism arises from such events as single nucleotide substitutions at the DNA level, different RNA processing, and dynamic enzymatic post-translational modifications. This minireview focuses on the measurement of intact proteins to describe the diversity found in proteomes.
Jeremiah D, Tipton +5 more
openaire +2 more sources
Microfluidic devices for protein analysis using intact and top‐down mass spectrometry
View, 2023 Top‐down mass spectrometry (TD‐MS) provides unique information on compositions, structures, and functions of proteins or protein complexes and has been recognized as a powerful approach to complement conventional tools in protein analysis.
Xinyang Shao, Yanyi Huang, Guanbo Wang
doaj +1 more source