Results 11 to 20 of about 106,020 (256)
Single-molecule protein identification by sub-nanopore sensors. [PDF]
PLoS Computational Biology, 2017 Recent advances in top-down mass spectrometry enabled identification of intact proteins, but this technology still faces challenges. For example, top-down mass spectrometry suffers from a lack of sensitivity since the ion counts for a single ...
Mikhail Kolmogorov +4 more
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Mass Spectrometry Analysis of Intact Proteins from Crude Samples [PDF]
Analytical Chemistry, 2020 Analysis of intact proteins by native mass spectrometry has emerged as a powerful tool for obtaining insight into subunit diversity, post-translational modifications, stoichiometry, structural arrangement, stability, and overall architecture. Typically, such an analysis is performed following protein purification procedures, which are time consuming ...
Shay Vimer +2 more
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Rapid MRSA detection via tandem mass spectrometry of the intact 80 kDa PBP2a resistance protein
Scientific Reports, 2021 Treatment of antibiotic-resistant infections is dependent on the detection of specific bacterial genes or proteins in clinical assays. Identification of methicillin-resistant Staphylococcus aureus (MRSA) is often accomplished through the detection of ...
Jason R. Neil +11 more
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Journal of Extracellular Vesicles, 2022 Extracellular vesicles (EVs) are blood‐borne messengers that coordinate signalling between different tissues and organs in the body. The specificity of such crosstalk is determined by preferential EV docking to target sites, as mediated through protein ...
Julia Bauzá‐Martinez +3 more
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Integrating mass spectrometry of intact protein complexes into structural proteomics [PDF]
PROTEOMICS, 2012 MS analysis of intact protein complexes has emerged as an established technology for assessing the composition and connectivity within dynamic, heterogeneous multiprotein complexes at low concentrations and in the context of mixtures. As this technology continues to move forward, one of the main challenges is to integrate the information content of ...
Hyung, Suk‐joon, Ruotolo, Brandon T.
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In situ structure and dynamics of an alphacoronavirus spike protein by cryo-ET and cryo-EM
Nature Communications, 2022 Hsu and co-workers integrate cryo-electron tomography, cryo-electron microscopy and mass spectrometry to reveal the structural polymorphism of a pig coronavirus spike protein within intact viral particles, and how glycosylation modulates the ...
Cheng-Yu Huang +14 more
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Ambient ionisation mass spectrometry for in situ analysis of intact proteins [PDF]
Journal of Mass Spectrometry, 2018 AbstractAmbient surface mass spectrometry is an emerging field which shows great promise for the analysis of biomolecules directly from their biological substrate. In this article, we describe ambient ionisation mass spectrometry techniques for the in situ analysis of intact proteins.
Klaudia I. Kocurek +2 more
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Electrospray‐ionization mass spectrometry of intact intrinsic membrane proteins [PDF]
Protein Science, 1998 AbstractMembrane proteins drive and mediate many essential cellular processes making them a vital section of the proteome. However, the amphipathic nature of these molecules ensures their detailed structural analysis remains challenging. A versatile procedure for effective electrospray‐ionization mass spectrometry (ESI‐MS) of intact intrinsic membrane ...
J P, Whitelegge +2 more
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Molecules, 2020 The effectiveness of a synthesized matrix, α-cyano-5-phenyl-2,4-pentadienic acid (CPPA), for protein analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) in complex samples such as foodstuff and ...
Antonio Monopoli +3 more
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Analysis of Bovine Kappa-Casein Glycomacropeptide by Liquid Chromatography–Tandem Mass Spectrometry
Foods, 2021 Caseinomacropeptide (CMP) is released from bovine kappa-casein after rennet treatment and is one of the major peptides in whey protein isolate. CMP has in vitro anti-inflammatory and antibacterial activities.
Yunyao Qu +3 more
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