Results 51 to 60 of about 106,020 (256)

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

Three phosphatase families form a community: The phosphohydrolases that act upon inositol pyrophosphates

FEBS Letters, EarlyView.
Inositol pyrophosphates are energy‐rich signaling molecules that perform critical functions in cells. Three different families of phosphatases hydrolyze the β phosphate of the inositol pyrophosphate molecules: two have narrow specificities and one is promiscuous.
Ronda J. Rolfes
wiley   +1 more source

On the utility of predictive chromatography to complement mass spectrometry based intact protein identification [PDF]

Analytical and Bioanalytical Chemistry, 2011
The amino acid sequence determines the individual protein three-dimensional structure and its functioning in an organism. Therefore, "reading" a protein sequence and determining its changes due to mutations or post-translational modifications is one of the objectives of proteomic experiments.
Pridatchenko, Marina L., Perlova, Tatyana Yu., Ben Hamidane, Hisham, Goloborodko, Anton A., Tarasova, Irina A., Gorshkov, Alexander V., Evreinov, Victor V., Tsybin, Yury O., Gorshkov, Mikhail V.   +8 more
openaire   +3 more sources

ABL kinase‐dependent phosphorylation of SH proteins promotes their direct interaction with CRK family SH2 domains

FEBS Letters, EarlyView.
CT10 regulator of kinase (CRK) and CRK‐Like (CRKL) are signaling adaptors driving cell adhesion, motility, differentiation, and proliferation. SH2‐domain containing (SH) proteins are enriched in YXXP motifs which when phosphorylated create preferred binding sites for CRK family SH2 domains.
Phoebe M. Cousens, Brendan W. Chandler, Anna M. Schmoker, Jaye L. Weinert, Charlotte A. Kearns, Warren T. Yacawych, Amila Šemić, Alicia M. Ebert, Bryan A. Ballif   +8 more
wiley   +1 more source

A Comprehensive Guide for Performing Sample Preparation and Top-Down Protein Analysis

Proteomes, 2017
Methodologies for the global analysis of proteins in a sample, or proteome analysis, have been available since 1975 when Patrick O′Farrell published the first paper describing two-dimensional gel electrophoresis (2D-PAGE).
Matthew P. Padula, Iain J. Berry, Matthew B. O′Rourke, Benjamin B.A. Raymond, Jerran Santos, Steven P. Djordjevic   +5 more
doaj   +1 more source

An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes

FEBS Letters, EarlyView.
GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg, Martine Hjeltnes, Josephine Krieger, Joshua E. Elias, Maria Stensland, Kristian Holm, Xiaojun Jiang, Chuki Khangsar, Tuula A. Nyman, Arthur Kaser, Johannes R. Hov, Tom H. Karlsen, Nicole C. Kaneider, Frode L. Jahnsen, Georg Schneditz   +14 more
wiley   +1 more source

Discrimination of different species from the genus Drosophila by intact protein profiling using matrix-assisted laser desorption ionization mass spectrometry

BMC Evolutionary Biology, 2010
Background The use of molecular biology-based methods for species identification and establishing phylogenetic relationships has supplanted traditional methods relying on morphological characteristics.
Gröger-Arndt Helke, Kalkhof Stefan, Görner Renate, Feltens Ralph, von Bergen Martin   +4 more
doaj   +1 more source

Best practices and benchmarks for intact protein analysis for top-down mass spectrometry [PDF]

Nature Methods, 2019
One gene can give rise to many functionally distinct proteoforms, each of which has a characteristic molecular mass. Top-down mass spectrometry enables the analysis of intact proteins and proteoforms. Here members of the Consortium for Top-Down Proteomics provide a decision tree that guides researchers to robust protocols for mass analysis of intact ...
Daniel P. Donnelly, Catherine M. Rawlins, Caroline J. DeHart, Luca Fornelli, Luis F. Schachner, Ziqing Lin, Jennifer L. Lippens, Krishna C. Aluri, Richa Sarin, Bifan Chen, Carter Lantz, Wonhyeuk Jung, Kendall R. Johnson, Antonius Koller, Jeremy J. Wolff, Iain D. G. Campuzano, Jared R. Auclair, Alexander R. Ivanov, Julian P. Whitelegge, Ljiljana Paša-Tolić, Julia Chamot-Rooke, Paul O. Danis, Lloyd M. Smith, Yury O. Tsybin, Joseph A. Loo, Ying Ge, Neil L. Kelleher, Jeffrey N. Agar   +27 more
openaire   +4 more sources

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

FEBS Letters, EarlyView.
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt, Ana‐Maria Sandru, Georg Vucak, Sebastian Didusch, Biljana Riemelmoser, Karin Ehrenreiter, Markus Hartl, Lukas A. Huber, Manuela Baccarini   +8 more
wiley   +1 more source

Extending the capabilities of intact-mass analyses to monoclonal immunoglobulins of the E-isotype (IgE)

mAbs, 2022
Mass spectrometry (MS) has become an indispensable tool in structural characterization and quality control of monoclonal antibodies (mAbs). Intact-mass analysis is a particularly attractive option that provides a powerful and cost-effective means to not ...
Wenhua Yang, Daniil G. Ivanov, Igor A. Kaltashov   +2 more
doaj   +1 more source