Results 91 to 100 of about 10,473 (233)
Desenvolvimento de sistemas de regulação da expressão gênica em leveduras baseados em optogenética e no uso de serina integrases [PDF]
, 2023 Tese (doutorado) — Universidade de Brasília, Instituto de Ciências Biológicas, Departamento de Biologia Celular, Programa de Pós-Graduação em Biologia Molecular, 2023.A levedura Komagataella phaffii (Pichia pastoris) tem sido utilizada como uma ...
Pérez, Ana Laura Alfonso
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Frontiers in Genome EditingAdvancements in genome editing technologies, notably CRISPR/Cas9, base editing (BE), and prime editing (PE), have revolutionized plant biotechnology, offering unprecedented precision in crop improvement to address the ongoing global warming challenge ...
Dylan Gallo +2 more
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Target DNA bending by the Mu transpososome promotes careful transposition and prevents its reversal
eLife, 2017 The transposition of bacteriophage Mu serves as a model system for understanding DDE transposases and integrases. All available structures of these enzymes at the end of the transposition reaction, including Mu, exhibit significant bends in the ...
James R Fuller, Phoebe A Rice
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Journal of Fish Diseases, EarlyView.ABSTRACT Monitoring antimicrobial resistance (AMR) in fish pathogens is essential for supporting sustainable aquaculture. In this study, susceptibility testing and genome sequencing were used to characterize the AMR profile of seven Flavobacterium psychrophilum originating from Danish rainbow trout farms (2019–2022).
Valentina L. Donati, Lone Madsen
wiley +1 more source
Medical Sciences ForumThe overuse of antibiotics in livestock contributes to antimicrobial resistance and zoonotic risk. This study investigated 19 Escherichia coli isolates from broiler feces (Savinor, Portugal), characterizing resistance genes, virulence factors, integrases,
Jessica Ribeiro +5 more
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Journal of Fish Diseases, EarlyView.ABSTRACT Piscine lactococcosis is an important bacterial disease of farmed fish. The causative agents, Lactococcus garvieae, Lactococcus petauri and Lactococcus formosensis, are closely related, which complicates species‐level identification. We developed a conventional multiplex PCR assay targeting species‐specific genes identified by comparative ...
Tomoaki Yoshino +9 more
wiley +1 more source
Journal of Phycology, EarlyView.Abstract Picocystis salinarum is an early‐diverging chlorophyte and the sole described member of the Picocystophyceae, frequently dominating hypersaline and alkaline lakes despite extreme physicochemical constraints. To elucidate the genomic foundations of its ecological success, we generated a fully annotated, chromosome‐scale nuclear genome assembly ...
Claude Lemieux +2 more
wiley +1 more source
DNA synapsis and cleavage assays with wild-type and mutant integrases.
, 2013 Labelled attB was incubated with wild-type or mutant integrases (Panel A:R18A, I141A; Panel B:L143A, E153A; Panel C:I432A) in the presence of no cold attP, 198 bp cold attP in binding buffer and then untreated or treated with proteinase K. Reactions were
Siman Peng (367675) +7 more
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Dispersion of Phage Integrases in Acinetobacter Baumannii Species
, 2014 Dispersion of Phage Integrases in Acinetobacter baumannii strains E. Stephanie Guardado1,2, German M. Traglia2, Dr. M. Soledad Ramirez2 1Biological Sciences Dept, Cal Poly Pomona 2Instituto de Microbiología y Parasitología Medica (IMPaM), Facultad de ...
Guardado, Eleana
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Photochemistry and Photobiology, EarlyView.UV irradiation drives metabolite complexity through photoisomerization of hydroxycinnamic acid‐containing compounds in plants. The olefin functional group of hydroxycinnamic acid enables structural plasticity through trans/cis isomerization. Multiple chromophores within a molecule can amplify UV‐induced transformations.
Mailane Rose Sekgobela +5 more
wiley +1 more source