Results 121 to 130 of about 10,473 (233)

Discovery and Engineering of a Rat Endogenous Retrovirus Reverse Transcriptase for Efficient Prime Editing

Advanced Science, Volume 13, Issue 49, 3 September 2026.
We screened 558 reverse transcriptases and engineered an optimized rat endogenous retrovirus‐derived variant, enRERV‐RT, via structure‐guided engineering and deep mutational scanning. This enhanced prime editor, based on the engineered RT, outperforms conventional M‐MLV‐RT systems across plant and animal cells, particularly at hard‐to‐edit loci ...
Linsha Ma, Pengcheng Yao, Shengqi Wu, Yuanyuan Shi, Lang Qin, Baitao Li, Jiayi Zhu, Minhua Huang, Yichong Zhu, Yuwen Song, Jinhuan Pang, Ziping Guo, Guochuan Wu, Chen Wang, Kewei Xu, Ruihua Huang, Quan Kuang, Liang Qu, Changtian Pan, Xianrong Xie, Qinlong Zhu, Jiaying Huang, Qiupeng Lin   +22 more
wiley   +1 more source

Large serine integrases utilise scavenged phage proteins as directionality cofactors [PDF]

Recombination directionality factors (RDFs) for large serine integrases (LSIs) are cofactor proteins that control the directionality of recombination to favour excision over insertion.
Reyes, TP, Alsaleh, A, Olorunniji, F, Baksh, A, Holland, A, Taiwo-Aiyerin, OT, Pigli, Y, Rice, PA, Shin, H   +8 more
core   +1 more source

Engineering CAR‐Macrophages With Advanced Delivery Systems for Tissue Repair

Advanced Science, Volume 13, Issue 51, 14 September 2026.
This review highlights how engineered macrophages equipped with chimeric antigen receptors (CAR) guide tissue repair by recognizing disease‐related targets, clearing harmful cells, and reshaping local immune environments. It summarizes macrophage biology, CAR design, delivery platforms, and functionalization strategies, and discusses emerging ...
Yixin Zhang, Jintian Yu, Liang Yuan, Ziliang Fu, Yiming Yao, Lingna Chen, Shixiong Chen, Aikemu Ainiwaer, Xiaolin Cui   +8 more
wiley   +1 more source

Suprasellar Desmoplastic Infantile Ganglioglioma in a 7‐Month‐Old Infant: A Rare Diagnostic Consideration in Infant Suprasellar Masses

Clinical Case Reports, Volume 14, Issue 9, September 2026.
ABSTRACT Desmoplastic infantile ganglioglioma should be considered when an infant presents with a suprasellar mass, nystagmus, and developmental delay. Deep midline location limits resection, so BRAF V600E testing matters: it can open a targeted treatment option when surgery cannot control the disease.
Tawfiq Zuhair Abdullah Allaylah
wiley   +1 more source

Bacterial Lysis Observation Over Time Assay (BLOTS) for High‐Throughput Isolation, Sequencing, and Characterization of Bacteriophages

Current Protocols, Volume 6, Issue 9, September 2026.
Abstract Phages remain severely under‐sampled, largely due to the limitations of current isolation methods, which are labor‐intensive, low‐throughput, and often restricted to a narrow set of bacterial hosts. To address this gap, we developed a robust and scalable field‐to‐genome workflow that leverages laboratory automation to screen environmental ...
Sean Jia Le Pang, Soon Keong Wee, Bobby Guo Wei Lee, Odelia Yap, Eric Peng Huat Yap   +4 more
wiley   +1 more source

Strategies and mechanisms of precision genome engineering: From gene editing to genome writing

iMetaOmics, Volume 3, Issue 3, September 2026.
In this review, we examined the progression of genome manipulation from stochastic nuclease‐mediated cutting toward precise editing and programmable genome writing. We discussed tools like multi‐kilobase RNA‐guided integrators and Artificial Intelligence (AI)‐designed effectors and showed how these advances enable researchers to treat genomes as ...
Kerui Huang, Jianhong Tian, Wenyan Zhao, Siqin Zhang, Lixuan Xiang, Meiqing Yang, Lu Liu, Yazhou Huang, Ningyun Zhang, Lei Sun, Peng Xie, Nanyu Kuang, Hong Zhou, Chengfeng Qiu, Jun Yan, Xiaocheng Jiang, Qiang Tu, Yun Wang, Haoliang Hu, Youming Zhang   +19 more
wiley   +1 more source

vB_CacS-HV1 as a Novel Pahexavirus Bacteriophage with Lytic and Anti-Biofilm Potential against Cutibacterium acnes

Microorganisms
Acne vulgaris is a prevalent chronic inflammatory skin disease, most common in adolescence and often persisting into adulthood, leading to severe physical and psychological impacts. The primary etiological factor is Cutibacterium acnes infection.
Xu Li, Wenyan Ding, Zicheng Li, Yi Yan, Yigang Tong, Jialiang Xu, Mengzhe Li   +6 more
doaj   +1 more source

Antarctic soil microbiomes encode structurally conserved and phylogenetically diverse beta‐lactamases

iMetaOmics, Volume 3, Issue 3, September 2026.
An integrative metagenomic framework combining sequence, structural, and functional inference reveals a phylogenetically diverse and structurally conserved repertoire of putative beta‐lactamases across Antarctic soil microbiomes, with predominance of class A and subclass B3 enzymes and limited but detectable associations with mobile genetic elements ...
José Coche‐Miranda, Patricio Arros, Nicolás Canales, Camilo Berríos‐Pastén, Gastón Azziz, Rosalba Lagos, Julieta Orlando, Francisco P. Chávez, Andrés E. Marcoleta   +8 more
wiley   +1 more source

Probing Resistance Mutations in Retroviral Integrases by Direct Measurement of Dolutegravir Fluorescence

, 2017
FDA-approved integrase strand transfer inhibitors (raltegravir, elvitegravir and dolutegravir) efficiently inhibit HIV-1 replication. Here, we present fluorescence properties of these inhibitors.
Eric Deprez, Delelis, Olivier, Simon, Françoise, Thierry, Eloïse, Eloïse Thierry, Olivier Delelis, Lebourgeois, Samuel, Samuel Lebourgeois, Françoise Simon, Deprez, Eric   +9 more
core   +1 more source

Viruses in the sediments around the trenches harbor diverse survival mechanisms

iMetaOmics, Volume 3, Issue 3, September 2026.
Deep‐sea trenches are the deepest and most isolated environments in the global ocean but the diversity and adaptation of the viral communities remain poorly characterized. In this study, 5563 viral operational taxonomic units (vOTUs) were identified from sediments of the Yap Trench and Western Caroline Basin.
Yue Su, Hao Yu, Shujuan Sun, Kaiyang Zheng, Ziyue Wang, Chen Gao, Guangxue Li, Xiaoshou Liu, Hongbing Shao, David Paez‐Espino, Andrew McMinn, Yantao Liang, Min Wang   +12 more
wiley   +1 more source