Results 81 to 90 of about 87,380 (302)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

An efficient method for DNA extraction from Cladosporioid fungi [PDF]

, 2010
We developed an efficient method for DNA extraction from Cladosporioid fungi, which are important fungal plant pathogens. The cell wall of Cladosporioid fungi is often melanized, which makes it difficult to extract DNA from their cells.
Abd-Elsalam, K.A., Bahkali, A.H., Moslem, M.A., Wit, P.J.G.M., de   +3 more
core   +2 more sources

Chameleon sequences reveal structural effects in proteins representing micelle‐like distribution of hydrophobicity

FEBS Open Bio, EarlyView.
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman, Katarzyna Stapor, Leszek Konieczny, Krzysztof Gądek, Piotr Nowakowski   +4 more
wiley   +1 more source

Exon 7 splicing of ERα predicts poor prognosis and increases phenotypic heterogeneity in luminal a subtype breast cancer

FEBS Open Bio, EarlyView.
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui, Taobo Hu, Thomson Christian Husein, Wai Hei Shek, Bingrong Chen, Depeng Wang, Shu Wang, Weichuan Yu, Xiaodan Fan, Mengping Long, Toyotaka Ishibashi   +10 more
wiley   +1 more source

Finding needles in haystacks: linking scientific names, reference specimens and molecular data for Fungi [PDF]

, 2014
DNA phylogenetic comparisons have shown that morphology-based species recognition often underestimates fungal diversity. Therefore, the need for accurate DNA sequence data, tied to both correct taxonomic names and clearly annotated specimen data, has ...
Abarenkov, K, Aime, MC, Ariyawansa, HA, Bidartondo, M, Boekhout, T, Buyck, B, Cai, Q, Cardinali, G, Chen, J, Crespo, A, Crous, PW, Damm, U, De Beer, ZW, Dentinger, BTM, Dieguez Uribeondo, J, Divakar, PK, Duenas, M, Duong, V, Feau, N, Federhen, S, Fliegerova, K, Garcia, MA, Ge, Z-W, Griffith, G, Groenewald, JZ, Groenewald, M, Grube, M, Gryzenhout, M, Gueidan, C, Guo, L, Hambleton, S, Hamelin, R, Hansen, K, Hofstetter, V, Hong, S-B, Houbraken, J, Hughes, K, Hyde, KD, Inderbitzin, P, Irinyi, L, Johnston, PR, Karunarathna, SC, Kirk, PM, Koljalg, U, Kovacs, GM, Kraichak, E, Krizsan, K, Kurtzman, CP, Larsson, K-H, Leavitt, S, Letcher, PM, Liimatainen, K, Liu, J-K, Lodge, DJ, Luangsa-ard, JJ, Lumbsch, HT, Maharachchikumbura, SSN, Manamgoda, D, Martin, MP, Meyer, W, Miller, AN, Minnis, AM, Moncalvo, J-M, Mule, G, Nakasone, KK, Nilsson, RH, Niskanen, T, Olariaga, I, Papp, T, Petkovits, T, Pino-Bodas, R, Powell, MJ, Raja, HA, Redecker, D, Robbertse, B, Robert, V, Sarmiento-Ramirez, JM, Schoch, CL, Seifert, KA, Shrestha, B, Stenroos, S, Stielow, B, Subbarao, KV, Suh, S-O, Tanaka, K, Tedersoo, L, Teresa Telleria, M, Udayanga, D, Untereiner, WA, Vagvoelgyi, C, Visagie, C, Voigt, K, Walker, DM, Weir, BS, Weiss, M, Wijayawardene, NN, Wingfield, MJ, Xu, JP, Yang, ZL, Zhang, N, Zhuang, W-Y   +100 more
core   +2 more sources

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

A molecular phylogenetic study of Deschampsia (Poaceae: Aveneae) inferred from nuclear ITS and plastid trnL sequence data: support for the recognition of Avenella and Vahlodea [PDF]

, 2007
The circumscription and phylogeny of Deschampsia were studied for the first time by parsimony analysis of nuclear ribosomal internal transcribed spacer (ITS) and plastid trnL intron sequences.
Chiapella, Jorge Oscar
core  

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

FEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source

Technical note: use of internal transcribed spacer for ruminal yeast identification in dairy cows

Animal, 2016
Molecular techniques are important tools for microbiological studies in different habitats, and the internal transcribed spacer (ITS) has been proved to be useful for analyzing fungal diversity. The aim of this study was to use the ITS region to generate
E. Vargas-Bello-Pérez, N. Cancino-Padilla, J. Romero   +2 more
doaj   +1 more source

Phylogenetic relationships of the Oxytropis campestris and Oxytropis arctica complexes in Alaska inferred from non-coding nuclear DNA and RAPD data [PDF]

, 2001
Thesis (M.S.) University of Alaska Fairbanks, 2001The taxonomy and evolutionary relationships of the Oxytropis arctica and Oxytropis campestris complexes in Alaska are poorly understood.
Jorgensen, Janet Lynn
core