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Human Intestinal Organoids: Promise and Challenge
Frontiers in Cell and Developmental Biology, 2022 The study of human intestinal biology in healthy and diseased conditions has always been challenging. Primary obstacles have included limited tissue accessibility, inadequate in vitro maintenance and ethical constrains.
Jasin Taelman +5 more
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Intestinal organoids: The path towards clinical application
European Journal of Cell BiologyOrganoids have revolutionized the whole field of biology with their ability to model complex three-dimensional human organs in vitro. Intestinal organoids were especially consequential as the first successful long-term culture of intestinal stem cells ...
Hady Yuki Sugihara +2 more
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Intestinal organoid cocultures with microbes [PDF]
Nature Protocols, 2021 Adult-stem-cell-derived organoids model human epithelial tissues ex vivo, which enables the study of host-microbe interactions with great experimental control. This protocol comprises methods to coculture organoids with microbes, particularly focusing on human small intestinal and colon organoids exposed to individual bacterial species.
Jens Puschhof +10 more
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Journal of Pediatric Gastroenterology and Nutrition, 2017 The development of sustainable intestinal organoid cell culture has emerged as a new modality for the study of intestinal function and cellular processes. Organoid culture is providing a new testbed for therapeutic research and development. Intestinal organoids, self-renewing 3-dimensional structures comprised intestinal stem cells and their ...
Wallach, Thomas E, Bayrer, James R
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Bioengineering for intestinal organoid cultures [PDF]
Current Opinion in Biotechnology, 2017 Recent advances allow access to human cell-based intestinal organoids that recreate human physiology to levels not possible with conventional 2D cell cultures. Despite their huge potential, there are many challenges that remain. This review will cover recent bioengineering approaches to improve organoid maturation, scale up, reproducibility and ...
Ge-Ah, Kim +2 more
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Microfluidic Device to Manipulate 3D Human Epithelial Cell-Derived Intestinal Organoids
Micromachines, 2022 In this study, we propose a microfluidic organoid-trapping device used to immobilize human intestinal organoids and apply fluidic stimuli to them. The proposed device has a microchannel with a trapping region with wall gaps between the channel walls and ...
Miki Matsumoto +3 more
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Intestinal organoids for Cystic Fibrosis research [PDF]
Journal of Cystic Fibrosis, 2020 Significant progress has been made in the development of CFTR modulator therapy; however, current CFTR modulator therapies are only available for a minority of the CF-patient population. Additionally, heterogeneity in in vivo modulator response has been reported among individuals carrying homozygous F508del-CFTR, adding to the desire for an optimal ...
E. de Poel, J.W. Lefferts, J.M. Beekman
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Animals, 2021 Intestinal organoids offer great promise for disease-modelling-based host–pathogen interactions and nutritional research for feed efficiency measurement in livestock and regenerative medicine for therapeutic purposes.
Bo-Ram Lee +8 more
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Veterinary Research, 2023 Chicken infection with Salmonella Typhimurium is an important source of foodborne human diseases. Salmonella colonizes the avian intestinal tract and more particularly the caecum, without causing symptoms.
Sonia Lacroix-Lamandé +7 more
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Frontiers in Molecular Biosciences, 2023 Intestinal organoids recapitulate many features of the in vivo gastrointestinal tract and have revolutionized in vitro studies of intestinal function and disease.
Panagiota Kakni +4 more
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