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Innervation of Human Intestinal Organoids
Journal of Visualized ExperimentsThe complexity of intestinal cytoarchitecture and function poses significant challenges for the creation of the bioengineered small intestine. Techniques for generating human intestinal organoids (HIOs) resembling human small intestine have been previously reported. HIOs contain epithelium and mesenchyme but lack other critical components of functional
Rachel C, Bordelon +2 more
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Intestinal organoid technology and applications in probiotics
Critical Reviews in Food Science and Nutrition, 2023The impacts of probiotics on maintaining the host's intestinal health have been extensively confirmed. Organoid technology revolutionizes intestinal health research by providing a unique platform to study the effects of probiotics. It overcomes challenges posed by animal models and 2D cell models in accurately simulating the in vivo environment.
Han Tan +6 more
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Generation of small intestinal organoids for experimental intestinal physiology
2020Human intestinal organoids (HIOs) derived from pluripotent stem cells were first described almost a decade ago as a method to differentiate intestinal tissue containing both epithelium and supporting mesenchymal cells. The original protocol documents a directed differentiation approach to first induce definitive endoderm from pluripotent stem cells ...
Meghan, Capeling +6 more
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Long-Term Culture of Intestinal Organoids
2018The in vitro long-term expansion of primary intestinal epithelial cells has been hampered by the inability to maintain an immature stem cell population. Recent technical advances have led to the development of a novel in vitro culture system that can sustain intestinal stem cells (ISCs) using growth factors that mimic the intestinal microenvironment in
Seung Bum, Lee +2 more
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Mouse Small Intestinal Organoid Cultures
The intestinal epithelium is a highly dynamic and self-renewing tissue that is crucial for maintaining gut homeostasis. It can be cultured in vitro from isolated crypts to form three-dimensional (3D) intestinal organoids. These organoids have the ability to proliferate and differentiate into various epithelial cell lineages, offering a more ...Lei, Chen, Xiaoting, Xu
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Optimization of Vascularized Intestinal Organoid Model
Advanced Healthcare MaterialsAbstractVasculature is crucial for maintaining organ homeostasis and metabolism. Although 3D organoids can mimic organ structures and patterns, they still lack vascular systems, limiting the recapitulation of physiological complexities. Although vascularization of organoids has been demonstrated by mixing Matrigel in fibrin, how the mixed gel niche ...
Zhang Wen +4 more
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Implantable synthetic organoid matrices for intestinal regeneration
Nature Cell Biology, 2017Organoids are a powerful tool to study both physiological and disease processes. A completely synthetic matrix assembled from exchangeable modular parts has been developed and not only supports proliferation of human intestinal organoids derived from pluripotent embryonic stem cells, but also augments subsequent ad vivo implantation into injured murine
Jeffrey W, Brown, Jason C, Mills
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Efficient Culture of Intestinal Organoids with Blebbistatin
2017The intestinal epithelium is one of the most rapidly self-renewing tissues throughout life in mammals. A small population of stem cells at the base of crypt in the epithelium can continually self-renew and give rise to differentiated epithelial cells.
Zhen, Qi, Ye-Guang, Chen
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Next-generation intestinal organoids
Nature Reviews Gastroenterology & Hepatology, 2020openaire +2 more sources