Results 31 to 40 of about 807,453 (347)

Evidence for the involvement of lipid rafts localized at the ER-mitochondria associated membranes in autophagosome formation [PDF]

, 2016
Mitochondria-associated membranes (MAMs) are subdomains of the endoplasmic reticulum (ER) that interact with mitochondria. This membrane scrambling between ER and mitochondria appears to play a critical role in the earliest steps of autophagy.
Faggioni, Alberto, Gambardella, Lucrezia, Garofalo, Tina, Malorni, Walter, Manganelli, Valeria, Marconi, Matteo, Matarrese, Paola, Misasi, Roberta, Sorice, Maurizio, Tinari, Antonella   +9 more
core   +2 more sources

Metal-tagging Transmission Electron Microscopy for Localisation of Tombusvirus Replication Compartments in Yeast

Bio-Protocol, 2018
Positive-stranded (+) RNA viruses are intracellular pathogens in humans, animals and plants. To build viral replicase complexes (VRCs) viruses manipulate lipid flows and reorganize subcellular membranes.
Isabel Fernandez de Castro, Cristina Risco   +1 more
doaj   +1 more source

A bacteriophage endolysin that eliminates intracellular streptococci

eLife, 2016
PlyC, a bacteriophage-encoded endolysin, lyses Streptococcus pyogenes (Spy) on contact. Here, we demonstrate that PlyC is a potent agent for controlling intracellular Spy that often underlies refractory infections.
Yang Shen, Marilia Barros, Tarek Vennemann, D Travis Gallagher, Yizhou Yin, Sara B Linden, Ryan D Heselpoth, Dennis J Spencer, David M Donovan, John Moult, Vincent A Fischetti, Frank Heinrich, Mathias Lösche, Daniel C Nelson   +13 more
doaj   +1 more source

Lipid Phosphate Phosphatases and Cancer

Biomolecules, 2020
Lipid phosphate phosphatases (LPPs) are a group of three enzymes (LPP1–3) that belong to a phospholipid phosphatase (PLPP) family. The LPPs dephosphorylate a wide spectrum of bioactive lipid phosphates, among which lysophosphatidate (LPA) and sphingosine
Xiaoyun Tang, David N. Brindley
doaj   +1 more source

Electropermeabilization of inner and outer cell membranes with microsecond pulsed electric field. Quantitative study with calcium ions [PDF]

, 2017
Microsecond pulsed electric fields (mu sPEF) permeabilize the plasma membrane (PM) and are widely used in research, medicine and biotechnology. For internal membranes permeabilization, nanosecond pulsed electric fields (nsPEF) are applied but this ...
Andrã©, Franck M., Denzi, Agnese, Hanna, Hanna, Liberti, Micaela, Mir, Lluis M.   +4 more
core   +2 more sources

Viruses and Lipids

Viruses, 2010
As obligatory intracellular pathogens, viruses exploit various cellular molecules and structures, such as cellular membranes, for their propagation.
Akira Ono
doaj   +1 more source

Functional selectivity of GPCR-directed drug action through location bias. [PDF]

, 2017
G-protein-coupled receptors (GPCRs) are increasingly recognized to operate from intracellular membranes as well as the plasma membrane. The β 2 -adrenergic GPCR can activate G s -linked cyclic AMP (G s -cAMP) signaling from endosomes.
Conti, Marco, Huang, Bo, Irannejad, Roshanak, Mika, Delphine, Pessino, Veronica, von Zastrow, Mark, Wedegaertner, Philip B.   +6 more
core   +3 more sources

Intracellular Membrane Traffic at High Resolution [PDF]

, 2010
Membrane traffic between organelles is essential for a multitude of processes that maintain cell homeostasis. Many steps in these tightly regulated trafficking pathways take place in microdomains on the membranes of organelles, which require analysis at nanometer resolution. Electron microscopy (EM) can visualize these processes in detail and is mainly
van Weering, JRT, Brown, E, Sharp, TH, Mantell, J, Cullen, PJ, Verkade, P   +5 more
openaire   +4 more sources

Evaluation of Reference Gene Stability for Investigations of Intracellular Signalling in Human Cancer and Non-Malignant Mesenchymal Stromal Cells

Frontiers in Bioscience-Scholar
Background: Real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR) is a powerful tool for analysing target gene expression in biological samples.
Vera Kosheverova, Alexander Schwarz, Rimma Kamentseva, Marianna Kharchenko, Elena Kornilova   +4 more
doaj   +1 more source

Contacting domains segregate a lipid transporter from a solute transporter in the malarial host–parasite interface

Nature Communications, 2020
While membrane contact sites between intracellular organelles are abundant, little is known about the contacts between membranes that delimit extracellular junctions within cells, such as intracellular parasites.
Matthias Garten, Josh R. Beck, Robyn Roth, Tatyana Tenkova-Heuser, John Heuser, Eva S. Istvan, Christopher K. E. Bleck, Daniel E. Goldberg, Joshua Zimmerberg   +8 more
doaj   +1 more source