Results 71 to 80 of about 82,193 (311)

pFAR plasmids: New Eukaryotic Expression Vectors for Gene Therapy, devoid of Antibiotic Resistance Markers [PDF]

, 2008
Efficient production of eukaryotic expression vectors requires the selection of plasmid-containing bacteria. To avoid the risk of dissemination of antibiotic resistance markers, we developed a new system to produce a family of plasmids Free of Antibiotic
Corinne Marie, Daniel Scherman, Gaelle Vandermeulen, Magali Richard, Mickael Quiviger, Veronique Preat   +5 more
core   +1 more source

Perfusion Bioreactor Culture Incorporating Mechanical Confinement Enhances Mesenchymal Stem Cell Extracellular Vesicle Production and Wound Healing Potential

Advanced Materials Technologies, EarlyView.
Scalable manufacturing challenges and limited potency remain two of the most intransigent issues limiting clinical translation of EV therapeutics. Mechanical cues can induce phenotypic changes and increased vesiculation in EV source cells without the use of extraneous reagents, chemicals, and/or cargo, thereby potentially drastically improving the ...
Emily H. Powsner, Raith Nowak, Stephanie M. Kronstadt, Nicholas H. Pirolli, Talia J. Solomon, Kristin Nikolov, William Pieper Holeman, Ian M. Smith, Kimberly M. Stroka, John P. Fisher, Steven M. Jay   +10 more
wiley   +1 more source

Vaccination Route as a Determinant of Protective Antibody Responses against Herpes Simplex Virus

Vaccines, 2020
Herpes simplex viruses (HSV) are significant global health problems associated with mucosal and neurologic disease. Prior experimental vaccines primarily elicited neutralizing antibodies targeting glycoprotein D (gD), but those that advanced to clinical ...
Clare Burn Aschner, Carl Pierce, David M. Knipe, Betsy C. Herold   +3 more
doaj   +1 more source

Safety and immunogenicity of intradermal administration of fractional dose CoronaVac®, ChAdOx1 nCoV-19 and BNT162b2 as primary series vaccination

Frontiers in Immunology, 2022
There is a limited supply of COVID-19 vaccines, with less than 20% of eligible populations in low-income countries having received one dose. Intradermal delivery of fractional dose vaccines is one way to improve global vaccine access, but no studies have
Somruedee Chatsiricharoenkul, Somruedee Chatsiricharoenkul, Suvimol Niyomnaitham, Suvimol Niyomnaitham, Harry Joshua Posen, Zheng Quan Toh, Zheng Quan Toh, Paul V. Licciardi, Paul V. Licciardi, Patimaporn Wongprompitak, Thaneeya Duangchinda, Thaneeya Duangchinda, Pattarakul Pakchotanon, Pattarakul Pakchotanon, Warangkana Chantima, Warangkana Chantima, Kulkanya Chokephaibulkit, Kulkanya Chokephaibulkit   +17 more
doaj   +1 more source

Characterization of the T cell receptor repertoire causing collagen arthritis in mice [PDF]

, 1993
Collagen type II-induced arthritis (CIA) is generated in susceptible rodent strains by intradermal injections of homologous or heterologous native type II collagen in complete Freund's adjuvant.
Hood, Leroy E., Kanagawa, Osami, Osman, Gamal-E, Toda, Masaaki   +3 more
core   +2 more sources

High Humidity Exacerbates Psoriasiform Skin Disease Relapse by Increasing Tissue‐Resident Memory T Cells via Altering Skin Microbiota

Advanced Science, EarlyView.
We demonstrated that high humidity worsened psoriasis relapse in murine psoriasiform skin inflammation by increasing skin‐resident memory CD8+ cells via upregulating IL‐15Rα on keratinocytes. The increases in IL‐15Rα and memory CD8+ cells were attributed to S. nepalensis and its metabolite ADMA in skin exposed to high humidity.
Chun‐Ling Liang, Yuchao Chen, Chuanjian Lu, Huazhen Liu, Fenglian Qin, Sisi Dai, Feifei Qiu, Haiming Chen, Weihui Lu, Jonathan S. Bromberg, Zhenhua Dai   +10 more
wiley   +1 more source

Penicillin Allergy Assessment and Skin Testing in the Outpatient Setting [PDF]

, 2019
Penicillin allergies are among of the most commonly reported allergies, yet only 10% of these patients are truly allergic. This leads to potential inadvertent negative consequences for patients and makes treatment decisions challenging for clinicians ...
Avery, Lisa M., Bookstaver, P. Brandon, Justo, Julie Ann, Kufel, Wesley D.   +3 more
core   +3 more sources

Pre‐Encoded IFN‐I Sensitivity Exacerbates Memory T Cell Senescence in Solid Tumors

Advanced Science, EarlyView.
Type I interferon (IFN‐I) signaling promotes p21‐dependent cell cycle arrest in senescent tumor‐specific memory T cells, resulting in poor proliferative responses and solid tumor regression during cancer vaccination. Conversely, IFNα/β receptor blockade reinvigorates T cell proliferation to regress solid tumors and is more effective with increasing ...
Andrew Nguyen, Scott R Walsh, Li Deng, Lan Chen, Yonghong Wan   +4 more
wiley   +1 more source

Intradermal delivery of a fractional dose of influenza H7N9 split vaccine elicits protective immunity in mice and rats

Human Vaccines & Immunotherapeutics, 2018
Vaccination is the most effective method of preventing the spread of the influenza virus. However, the traditional intramuscular (IM) immunization causes fear, pain, and cross infection.
Shanshan Zhou, Tianyu Ren, Hongjing Gu, Cheng Wang, Min Li, Zhongpeng Zhao, Li Xing, Liangyan Zhang, Yi Sun, Penghui Yang, Xiliang Wang   +10 more
doaj   +1 more source

Localized NF‐κB Inhibition Reduces Lipid Nanoparticle‐Associated Inflammation

Advanced Science, EarlyView.
Lipid nanoparticles (LNPs) activate NF‐κB and provoke inflammation that limits therapeutic delivery. By encapsulating selective NF‐κB inhibitors within LNPs, localized suppression of inflammatory signaling is achieved without compromising mRNA expression.
Carolann L. Espy, Jichuan Wu, Serena Omo‐Lamai, Liuqian Wang, Fengyi Dong, Aleksa Milosavljevic, Sarah O'Neill, Taylor Brysgel, Yufei Wang, Michael Zaleski, Emily Wolfe, Rhea Maheshwari, Manthan Patel, Dirk Trauner, Jia Nong, Zhicheng Wang, Jacob S. Brenner   +16 more
wiley   +1 more source