Results 181 to 190 of about 5,123 (214)

Intrinsically Disordered Proteins (IDP): Purification Under Denaturing Conditions

2022
Recombinant protein expression in E. coli often induces the expressed protein to accumulate in insoluble aggregates, named inclusion bodies (IBs), that represent easy to isolate, highly pure protein reservoirs. IBs can be solubilized by denaturing agents but this procedure requires, for complex globular proteins, a refolding step that can be ...
Mireia, Pesarrodona, Isabel, Latorre, Xavier, Salvatella   +2 more
openaire   +2 more sources

Intrinsically disordered proteins (IDP) and metal ions: the case of amylin (HiAPP)

SMART eLAB, 2021
poster presented at 1st Conference on Crystallography, Structural Chemistry and Biosystems, (Catania)
Macrì, Antonio, Tabbì, Giovanni, Attanasio, Francesco, Di Natale, Giuseppe, La Mendola, Diego, Rizzarelli, Enrico   +5 more
openaire   +1 more source

IDP-EDL: enhancing intrinsically disordered protein prediction by combining protein language model and ensemble deep learning

Briefings in Bioinformatics
Abstract Identification of intrinsically disordered regions (IDRs) in proteins is essential for understanding fundamental cellular processes. The IDRs can be divided into long disordered regions (LDRs) and short disordered regions (SDRs) according to their lengths.
Xiaopeng Jin, Hang Wei, Saisai Sun
exaly   +3 more sources

[Intrinsically disordered proteins (IDPs) and the impact on cell stress resistance].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2022
Intrinsically disordered proteins (IDPs) are proteins or protein regions that fail to get folded into definite three-dimensional structures but participate in various biological processes and perform specific functions. Defying the traditional protein "sequence-structure-function" paradigm, they enrich the protein "structure-function" diversity ...
Ning, Yan, Hongxing, Li, Longhao, Wu, Shuo, Yang, Lujiang, Hao, Xiaoming, Bao   +5 more
openaire   +1 more source

Molecular Bases of Salinity Resistance via Intrinsic Disordered Protein (IDP)

2023
Salt-affected soil is a prominent challenge in agriculture. Nowadays, more than 800 million hectares of land (about 6% of the world’s total land area) are induced with high salt concentrations, and thus, are unsuitable for growing typically salt-sensitive crop plants. The ongoing salinization of arable land exacerbates this limitation.
openaire   +2 more sources

Targeting protein–protein interactions (PPIs) of transcription factors: Challenges of intrinsically disordered proteins (IDPs) and regions (IDRs)

Progress in Biophysics and Molecular Biology, 2015
In this review we discuss recent progress in targeting the protein-protein interactions made by oncogenic transcription factors. We particularly focus on the challenges posed by the prevalence of intrinsically disordered regions in this class of protein and the strategies being used to overcome them.
Susan Sammak, Giovanna Zinzalla
openaire   +3 more sources

Intrinsically disordered proteins (IDPs) studied by EPR and in-cell EPR

2018
Intrinsically disordered proteins (IDPs) play important physiological, but also disease-related roles. In order to understand the function and malfunction of proteins of this class, electron paramagnetic resonance (EPR) spectroscopy has proven to be a valuable tool, allowing investigation of the protein structural ensembles upon interaction with the ...
Sabrina Weickert, Julia Cattani, Malte Drescher   +2 more
openaire   +1 more source

Exclusively Heteronuclear ¹³C‐Detected Amino‐Acid‐Selective NMR Experiments for the Study of Intrinsically Disordered Proteins (IDPs)

ChemBioChem, 2012
AbstractCarbon‐13 direct‐detection NMR methods have proved to be very useful for the characterization of intrinsically disordered proteins (IDPs). Here we present a suite of experiments in which amino‐acid‐selective editing blocks are encoded in CACON‐ and CANCO‐type sequences to give 13C‐detected spectra containing correlations arising from a ...
W. Bermel, BERTINI, IVANO, J. Chill, FELLI, ISABELLA CATERINA, N. Haba, MACHOHALLY VENKATESHAIAH, VASANTHA KUMAR, PIERATTELLI, ROBERTA   +6 more
openaire   +3 more sources

The gene expression profiling of hepatocellular carcinoma by a network analysis approach shows a dominance of intrinsically disordered proteins (IDPs) between hub nodes

Molecular BioSystems, 2015
Abstract We have analyzed the transcriptomic data from patients with hepatocellular carcinoma (HCC) after viral HCV infection at the various stages of the disease by means of a networking analysis using the publicly available E-MTAB-950 dataset.
Singh S, Colonna G, DI BERNARDO, Giovanni, Bergantino F, CAMMAROTA, Marcella, Castello G, Costantini S.   +6 more
openaire   +2 more sources

Longitudinal relaxation properties of 1HN and 1Hα determined by direct-detected 13C NMR experiments to study intrinsically disordered proteins (IDPs)

Journal of Magnetic Resonance, 2015
Intrinsically disordered proteins (IDPs) are functional proteins containing large fragments characterized by high local mobility. Bioinformatic studies have suggested that a significant fraction (more than 30%) of eukaryotic proteins has disordered regions of more than 50 amino acids in length.
HOSEK, TOMAS, Gil Caballero, Sergi, PIERATTELLI, ROBERTA, Brutscher, Bernhard, FELLI, ISABELLA CATERINA   +4 more
openaire   +3 more sources