Results 81 to 90 of about 29,703 (304)

Interpreting the effects of DNA polymerase variants at the structural level

Molecular Oncology, EarlyView.
Using MAVISp and molecular dynamics simulations, we analyzed over 60 000 missense variants in POLE and POLD1 from ClinVar, COSMIC, cBioPortal, and saturation mutagenesis. Identified mechanistic indicators, including stability, binding, and long‐range, enable structural interpretation, providing ACMG‐like evidence for possible reclassification of VUS ...
Matteo Arnaudi, Karolina Krzesińska, Ludovica Beltrame, Pablo Sánchez‐Izquierdo Besora, Matteo Tiberti, Mef Nilbert, Anna Rohlin, Elena Papaleo   +7 more
wiley   +1 more source

Structural Disorder Provides Increased Adaptability for Vesicle Trafficking Pathways

, 2013
Vesicle trafficking systems play essential roles in the communication between the organelles of eukaryotic cells and also between cells and their environment.
Peter Tompa, Rita Pancsa (173029), Natalia Pietrosemoli (280470), Pancsa Rita, Tompa Peter, Peter Tompa (38445), Pietrosemoli Natalia, Tompa, Péter, Rita Pancsa, Pietrosemoli, Natalia, Natalia Pietrosemoli, Pancsa, Rita   +11 more
core   +1 more source

Protocol for Investigating the Interactions Between Intrinsically Disordered Proteins and Membranes by Neutron Reflectometry

, 2020
Several intrinsically disordered proteins (IDPs) exhibit high affinity for lipid membranes. Among the different biophysical methods to probe protein-lipid interaction, neutron reflectometry (NR) can provide direct and structural detailed information on ...
Luchini A, Arleth L, Arleth, Lise, Lise Arleth, Luchini, Alessandra, Alessandra Luchini   +5 more
core   +1 more source

Functional Segments on Intrinsically Disordered Regions in Disease-Related Proteins [PDF]

Biomolecules, 2019
One of the unique characteristics of intrinsically disordered proteins (IPDs) is the existence of functional segments in intrinsically disordered regions (IDRs). A typical function of these segments is binding to partner molecules, such as proteins and DNAs. These segments play important roles in signaling pathways and transcriptional regulation.
Hiroto Anbo, Masaya Sato, Atsushi Okoshi, Satoshi Fukuchi   +3 more
openaire   +3 more sources

Intra- and inter-molecular regulation by intrinsically-disordered regions governs PUF protein RNA binding

Nature Communications, 2023
PUF proteins are characterized by globular RNA-binding domains. They also interact with partner proteins that modulate their RNA-binding activities. Caenorhabditis elegans PUF protein fem-3 binding factor-2 (FBF-2) partners with intrinsically disordered ...
Chen Qiu, Zihan Zhang, Robert N. Wine, Zachary T. Campbell, Jun Zhang, Traci M. Tanaka Hall   +5 more
doaj   +1 more source

The ‘Shape-Shifter’ Peptide from the Disulphide Isomerase PmScsC Shows Context-Dependent Conformational Preferences

Biomolecules, 2021
Multiple crystal structures of the homo-trimeric protein disulphide isomerase PmScsC reveal that the peptide which links the trimerization stalk and catalytic domain can adopt helical, β-strand and loop conformations. This region has been called a ‘shape-
Lorna J. Smith, Chloe W. Green, Christina Redfield   +2 more
doaj   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Intrinsic structural disorder in cytoskeletal proteins.

, 2013
Cytoskeleton, the internal scaffold of the cell, displays an exceptional combination of stability and dynamics. It is composed of three major filamentous networks, microfilaments (actin filaments), intermediate filaments (neurofilaments), and ...
Szabó, Beáta, Guharoy, Majnak, Martos, Sara Contreras, Tompa, Péter   +3 more
core   +1 more source

A Method for Systematic Assessment of Intrinsically Disordered Protein Regions by NMR [PDF]

International Journal of Molecular Sciences, 2015
Intrinsically disordered proteins (IDPs) that lack stable conformations and are highly flexible have attracted the attention of biologists. Therefore, the development of a systematic method to identify polypeptide regions that are unstructured in solution is important.
Natsuko Goda, Kana Shimizu, Yohta Kuwahara, Takeshi Tenno, Tamotsu Noguchi, Takahisa Ikegami, Motonori Ota, Hidekazu Hiroaki   +7 more
openaire   +2 more sources

CARs-DB: A Database of Cryptic Amyloidogenic Regions in Intrinsically Disordered Proteins

Frontiers in Molecular Biosciences, 2022
Proteome-wide analyses suggest that most globular proteins contain at least one amyloidogenic region, whereas these aggregation-prone segments are thought to be underrepresented in intrinsically disordered proteins (IDPs). In recent work, we reported that intrinsically disordered regions (IDRs) indeed sustain a significant amyloid load in the form of ...
Pintado-Grima, Carlos, Bárcenas, Oriol, Manglano-Artuñedo, Zoe, Vilaça, Rita, Macedo-Ribeiro, Sandra, Pallarès, Irantzu, Santos, Jaime, Ventura, Salvador   +7 more
openaire   +4 more sources