Results 211 to 220 of about 23,829 (240)

Intrinsically Disordered Proteins (IDP): Purification Under Denaturing Conditions

2022
Recombinant protein expression in E. coli often induces the expressed protein to accumulate in insoluble aggregates, named inclusion bodies (IBs), that represent easy to isolate, highly pure protein reservoirs. IBs can be solubilized by denaturing agents but this procedure requires, for complex globular proteins, a refolding step that can be ...
Mireia, Pesarrodona, Isabel, Latorre, Xavier, Salvatella   +2 more
openaire   +2 more sources

Intrinsically disordered proteins (IDP) and metal ions: the case of amylin (HiAPP)

SMART eLAB, 2021
poster presented at 1st Conference on Crystallography, Structural Chemistry and Biosystems, (Catania)
Macrì, Antonio, Tabbì, Giovanni, Attanasio, Francesco, Di Natale, Giuseppe, La Mendola, Diego, Rizzarelli, Enrico   +5 more
openaire   +1 more source

Rational drug design targeting intrinsically disordered proteins

WIREs Computational Molecular Science, 2023
Intrinsically disordered proteins (IDPs) are proteins that perform important biological functions without well‐defined structures under physiological conditions.
Hanping Wang, Ruoyao Xiong, L. Lai
semanticscholar   +1 more source

Dynamics and interactions of intrinsically disordered proteins.

Current Opinion in Structural Biology, 2023
Intrinsically disordered proteins (IDPs) are widespread in eukaryotes and participate in a variety of important cellular processes. Numerous studies using state-of-the-art experimental and theoretical methods have advanced our understanding of IDPs and ...
M. Arai, Shunji Suetaka, Koji Ooka
semanticscholar   +1 more source

Longitudinal relaxation properties of 1HN and 1Hα determined by direct-detected 13C NMR experiments to study intrinsically disordered proteins (IDPs)

Journal of Magnetic Resonance, 2015
Intrinsically disordered proteins (IDPs) are functional proteins containing large fragments characterized by high local mobility. Bioinformatic studies have suggested that a significant fraction (more than 30%) of eukaryotic proteins has disordered regions of more than 50 amino acids in length.
Tomáš Hosek, Sérgio Gil-Caballero, Roberta Pierattelli   +2 more
exaly   +4 more sources

Molecular Bases of Salinity Resistance via Intrinsic Disordered Protein (IDP)

2023
Salt-affected soil is a prominent challenge in agriculture. Nowadays, more than 800 million hectares of land (about 6% of the world’s total land area) are induced with high salt concentrations, and thus, are unsuitable for growing typically salt-sensitive crop plants. The ongoing salinization of arable land exacerbates this limitation.
openaire   +2 more sources

A multi-responsive intrinsically disordered protein (IDP)-directed green synthesis of fluorescent gold nanoclusters

Journal of Materials Chemistry B, 2015
Herein we demonstrate the green synthesis of fluorescent gold nanoclusters (AuNCs) using a multi-responsive intrinsically disordered protein (IDP) polymer, Rec1-resilin, as a multi-functional template.
Balu, R., Bourgeois, L., Elvin, C., Hill, A., Choudhury, N., Dutta, N.   +5 more
openaire   +5 more sources

Binding mechanisms of intrinsically disordered proteins: Insights from experimental studies and structural predictions.

Current Opinion in Structural Biology
Advances in the characterization of intrinsically disordered proteins (IDPs) have unveiled a remarkably complex and diverse interaction landscape, including coupled folding and binding, highly dynamic complexes, multivalent interactions, and even ...
Thibault Orand, M. R. Jensen
semanticscholar   +1 more source

Sizes, conformational fluctuations, and SAXS profiles for Intrinsically Disordered Proteins

bioRxiv
The preponderance of Intrinsically Disordered Proteins (IDPs) in the eukaryotic proteome, and their ability to interact with each other, folded proteins, RNA, and DNA for functional purposes, have made it important to quantitatively characterize their ...
M. Mugnai, Debayan Chakraborty, H. Nguyen, Farkhad Maksudov, Abhinaw Kumar, Wade F. Zeno, J. Stachowiak, J. Straub, D. Thirumalai   +8 more
semanticscholar   +1 more source

The gene expression profiling of hepatocellular carcinoma by a network analysis approach shows a dominance of intrinsically disordered proteins (IDPs) between hub nodes

Molecular BioSystems, 2015
Abstract We have analyzed the transcriptomic data from patients with hepatocellular carcinoma (HCC) after viral HCV infection at the various stages of the disease by means of a networking analysis using the publicly available E-MTAB-950 dataset.
Singh S, Colonna G, DI BERNARDO, Giovanni, Bergantino F, CAMMAROTA, Marcella, Castello G, Costantini S.   +6 more
openaire   +2 more sources