Results 71 to 80 of about 23,171 (195)

The Sherpa hypothesis: Phenotype-Preserving Disordered Proteins stabilize the phenotypes of neurons and oligodendrocytes

npj Systems Biology and Applications, 2023
Intrinsically disordered proteins (IDPs), which can interact with many partner proteins, are central to many physiological functions and to various pathologies that include neurodegeneration. Here, we introduce the Sherpa hypothesis, according to which a
Vic Norris, Judit Oláh, Sergey N. Krylov, Vladimir N. Uversky, Judit Ovádi   +4 more
doaj   +1 more source

Molecular Simulations Integrated with Experiments for Probing the Interaction Dynamics and Binding Mechanisms of Intrinsically Disordered Proteins

Current Opinion in Structural Biology, 2023
Intrinsically disordered proteins (IDPs) exploit their plasticity to deploy a rich panoply of soft interactions and binding phenomena. Advances in tailoring molecular simulations for IDPs combined with experimental cross-validation offer an atomistic ...
Catherine Ghosh, Suhani Nagpal, Victor Muñoz   +2 more
semanticscholar   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Modifications of Lysine Residues 294 and 311 Within the Microtubule‐Binding Region Alter Tau Aggregation and Tubulin Binding Equilibria

Angewandte Chemie, Volume 138, Issue 43, 19 October 2026.
The molecular consequences of specific lysine modifications in Alzheimer´s disease remain insufficiently resolved in the context of full‐length protein. Here we integrate protein semisynthesis, segmental isotope labelling, and high‐resolution NMR spectroscopy to achieve residue‐resolved interrogation of site‐specific acetylation and carboxymethylation.
Dominik P. Vogl, Mario Migotti, Robert Konrat, Anne C. Conibear, Christian F. W. Becker   +4 more
wiley   +2 more sources

Intrinsically Disordered Side of the Zika Virus Proteome

Frontiers in Cellular and Infection Microbiology, 2016
Over the last few decades, concepts of protein intrinsic disorder have been implicated in different biological processes. Recent studies have suggested that intrinsically disordered proteins (IDPs) provide structural plasticity and functional diversity ...
Rajanish Giri, Deepak Kumar, Nitin Sharma, Vladimir N. Uversky   +3 more
doaj   +1 more source

Accurate model of liquid–liquid phase behavior of intrinsically disordered proteins from optimization of single-chain properties

Proceedings of the National Academy of Sciences of the United States of America, 2021
Significance Cells may compartmentalize proteins via a demixing process known as liquid–liquid phase separation (LLPS), which is often driven by intrinsically disordered proteins (IDPs) and regions.
G. Tesei, Thea K. Schulze, R. Crehuet, K. Lindorff-Larsen   +3 more
semanticscholar   +1 more source

Structural Characterization of Intrinsically Disordered Proteins by NMR Spectroscopy

Molecules, 2013
Recent advances in NMR methodology and techniques allow the structural investigation of biomolecules of increasing size with atomic resolution. NMR spectroscopy is especially well-suited for the study of intrinsically disordered proteins (IDPs) and ...
Peter Tompa, Cesyen Cedeño, Simone Kosol, Sara Contreras-Martos   +3 more
doaj   +1 more source

DisProt in 2026: enhancing intrinsically disordered proteins accessibility, deposition, and annotation

Nucleic Acids Research
DisProt (https://disprot.org/) is an open database integrating experimental evidence on intrinsically disordered proteins (IDPs), intrinsically disordered regions (IDRs), and their functions.
María Victoria Nugnes, Kamel Eddine Adel Bouhraoua, Mehdi Zoubiri, R. Pancsa, Erzsébet Fichó, A. Monzon, Ana M. Melo, Edoardo Salladini, E. Leonardi, F. Quaglia, Daniyal Nasiribavil, Hamidreza Ghafouri, G. Julien, E. Pasche, Patrick Ruch, Paul Van Rijen, László Dobson, M. Schiavina, Trinidad Cordero, Zsofia E. Kalman, X. Castro, Valentín Iglesias, István Reményi, M. Mehdiabadi, Gábor Erdős, Z. Dosztányi, Peter Tompa, D. Piovesan, S. Tosatto, M. C. Aspromonte   +29 more
semanticscholar   +1 more source

Chaotic aging: Intrinsically disordered proteins in aging-related processes

bioRxiv, 2023
The development of aging is associated with the disruption of key cellular processes manifested as well-established hallmarks of aging. Intrinsically disordered proteins (IDPs) and intrinsically disordered regions (IDRs) have no stable tertiary structure
Vladimir D. Manyilov, N. Ilyinsky, S. Nesterov, B. M. Saqr, Guy W. Dayhoff, Egor V. Zinovev, Simon S. Matrenok, A. Fonin, I. Kuznetsova, K. K. Turoverov, V. Ivanovich, V. Uversky   +11 more
semanticscholar   +1 more source

Diverging Liquid–Liquid Phase Separation Behavior of Different Recombinant Major Ampullate Spidroins

Advanced Science, EarlyView.
Fibers spun from the MaSp1‐derivative as well as MaSp1‐MaSp2‐mixtures provide a deeper insight into the assembly process of the spidroins and how hierarchical architectures of spider silk fibers could be achieved. ABSTRACT Spider silk fibers show exceptional mechanical properties, based on their underlying spider silk proteins (spidroins) as well as a ...
Tim Schiller, Cheryl Hayashi, Thomas R. Scheibel   +2 more
wiley   +1 more source