Results 91 to 100 of about 1,046,746 (344)

Evolutionary dynamics of the chloroplast genome in Daphne (Thymelaeaceae): comparative analysis with related genera and insights into phylogenetics

open access: yesFEBS Open Bio, EarlyView.
Comparative analysis of chloroplast genomes from 14 genera of Thymelaeaceae revealed variation in gene content, ranging from 128 to 142 genes, primarily influenced by IR expansion/contraction events and pseudogenization of ndhF, ndhI, and ndhG. Two large inversions were detected within the large single‐copy region, including a synapomorphic inversion ...
Abdullah   +8 more
wiley   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

open access: yesFEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz   +6 more
wiley   +1 more source

Next-generation sequencing yields the complete chloroplast genome of Abies Alba

open access: yesMitochondrial DNA. Part B. Resources, 2019
Abies alba is native to the mountains of Europe and often used as Christmas trees. In this study, we reported the complete chloroplast genome of A. alba. The complete chloroplast genome is 121,243 bp in length, comprising a large single copy (LSC) region
Guo-Yin Li   +9 more
doaj   +1 more source

KLK7 overexpression promotes an aggressive phenotype and facilitates peritoneal dissemination in colorectal cancer cells

open access: yesFEBS Open Bio, EarlyView.
KLK7, a tissue kallikrein‐related peptidase, is elevated in advanced colorectal cancer and associated with shorter survival. High KLK7 levels in ascites correlate with peritoneal metastasis. In mice, KLK7 overexpression increases metastasis. In vitro, KLK7 enhances cancer cell proliferation, migration, adhesion, and spheroid formation, driving ...
Yosr Z. Haffani   +6 more
wiley   +1 more source

Next-generation sequencing yields the complete chloroplast genome of Abies fanjingshanensis, an Endangered species from South China

open access: yesMitochondrial DNA. Part B. Resources, 2019
Abies fansipanensis is an Endangered species in South China with less than 2500 mature trees. In this study, we reported the complete chloroplast genome of A. fansipanensis.
Zhen-Ni Guo   +4 more
doaj   +1 more source

Mobility and Generation of Mosaic Non-Autonomous Transposons by Tn3-Derived Inverted-Repeat Miniature Elements (TIMEs)

open access: yesPLoS ONE, 2014
Functional transposable elements (TEs) of several Pseudomonas spp. strains isolated from black shale ore of Lubin mine and from post-flotation tailings of Zelazny Most in Poland, were identified using a positive selection trap plasmid strategy.
M. Szuplewska   +4 more
semanticscholar   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

DNA transposons mediate duplications via transposition-independent and -dependent mechanisms in metazoans

open access: yesNature Communications, 2021
Transposons are accepted as evolutionary catalysts but how they do so remains less clear. Analyzing 100 animal genomes finds that terminal inverted repeat-type transposable elements catalyze new gene structures and new genes in animals via both ...
Shengjun Tan   +17 more
doaj   +1 more source

The evolution of short inverted repeats

open access: yes, 2020
Poster 1178B - TAGC ...
Bar Lavi   +2 more
openaire   +1 more source

HIV‐1 establishes immediate latency in T cells expressing the viral Nef protein

open access: yesFEBS Open Bio, EarlyView.
Nef is a viral protein often omitted from HIV‐1 reporter viruses. Consequently, its role in viral latency is unclear. We developed three novel dual reporter HIV‐1 derivatives that express Nef and allow for detection of latent and productive infection. Using these reporters, we show that Nef does not affect the establishment of immediate viral latency ...
Cindy Lam, Ivan Sadowski
wiley   +1 more source

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