The complete chloroplast genome of Coffea liberica (Gentianales: Rubiaceae)
Coffee is one of the most popular beverages around the world. As one of the best-known coffee species, Liberian coffee (Coffea liberica Bull ex Hiern 1876) has a high resistance to leaf rust, a devasting disease caused by Hemileia vastatrix.
Xuehui Bai+8 more
doaj +1 more source
Binding of the Tn3 transposase to the inverted repeats of Tn3
The transposase protein and the inverted repeat sequences of Tn3 are both essential for Tn3 cointegrate formation and transposition. We have developed two assays to detect site-specific binding of transposase to the inverted repeats: (1) a nitrocellulose filter binding assay in which transposase preferentially retains DNA fragments containing inverted ...
Angela K. Eggleston+2 more
openaire +3 more sources
Inverted DNA repeats: a source of eukaryotic genomic instability. [PDF]
While inverted DNA repeats are generally acknowledged to be an important source of genetic instability in prokaryotes, relatively little is known about their effects in eukaryotes. Using bacterial transposon Tn5 and its derivatives, we demonstrate that long inverted repeats also cause genetic instability leading to deletion in the yeast Saccharomyces ...
Kirill S. Lobachev+5 more
openaire +3 more sources
SARS‐CoV‐2 Is Linked to Brain Volume Loss in Multiple Sclerosis
ABSTRACT Objective The impact of SARS‐CoV‐2 infection on brain and spinal cord pathology in patients with multiple sclerosis (pwMS) remains unclear. We aimed to describe changes in brain lesion activity and brain and spinal cord volumes following SARS‐CoV‐2 infection.
Tomas Uher+12 more
wiley +1 more source
Single tube, high throughput cloning of inverted repeat constructs for double-stranded RNA expression. [PDF]
BACKGROUND: RNA interference (RNAi) has emerged as a powerful tool for the targeted knockout of genes for functional genomics, system biology studies and drug discovery applications.
Brian Hauge+4 more
doaj +1 more source
Isolation and characterization of an insertion element-like repetitive sequence specific for Mycobacterium tuberculosis complex [PDF]
We report the characterization of an insertion-like repetitive sequence containing the clone of Mycobacterium tuberculosis. This repetitive sequence contains seven inverted repeats.
Narayanan, P R+2 more
core
ABSTRACT Objective The cervical spinal cord (cSC) is highly relevant to clinical dysfunction in multiple sclerosis (MS) but remains understudied using quantitative magnetic resonance imaging (MRI). We assessed magnetization transfer ratio (MTR), a semi‐quantitative MRI measure sensitive to MS‐related tissue microstructural changes, in the cSC and its ...
Lisa Eunyoung Lee+26 more
wiley +1 more source
Repetitive DNA profile of the amphibian mitogenome
Background Repetitive DNA elements such as direct and inverted repeat sequences are present in every genome, playing numerous biological roles. In amphibians, the functions and effects of the repeat sequences have not been extensively explored.
Noel Cabañas+5 more
doaj +1 more source
Efficient cloning system for construction of gene silencing vectors in Aspergillus niger [PDF]
An approach based on Gateway recombination technology to efficiently construct silencing vectors was developed for use in the biotechnologically important fungus Aspergillus niger. The transcription activator of xylanolytic and cellulolytic genes XlnR of
Graaff, L.H., de+3 more
core +2 more sources
Understanding Mechanisms of Whole Brain and Regional Grey Matter Atrophy in Children With MOGAD
ABSTRACT Objective To investigate the mechanisms driving whole brain and regional grey matter (GM) volume changes along with their clinical correlates in paediatric myelin oligodendrocyte glycoprotein antibody (MOG‐Ab)–associated disease (MOGAD). Methods One‐hundred‐nine paediatric MOGAD patients from two UK centres underwent MRI at attack nadir and ...
Ermelinda De Meo+14 more
wiley +1 more source