Investigating the DNA-Binding Site for VirB, a Key Transcriptional Regulator of Shigella Virulence Genes, Using an In Vivo Binding Tool [PDF]
, 2019 The transcriptional anti-silencing and DNA-binding protein, VirB, is essential for the virulence of Shigella species and, yet, sequences required for VirB-DNA binding are poorly understood.
Karabachev, Alexander D. +6 more
core +2 more sources
Transduplication resulted in the incorporation of two protein-coding sequences into the Turmoil-1 transposable element of C. elegans [PDF]
, 2008 Transposable elements may acquire unrelated gene fragments into their sequences in a process called transduplication. Transduplication of protein-coding genes is common in plants, but is unknown of in animals.
Ast, Gil +4 more
core +4 more sources
Lack of Z-DNA Conformation in Mitomycin-Modified Polynucleotides Having Inverted Circular Dichroism [PDF]
, 1983 Poly(dG-dC)· poly(dG-dC) and Micrococcus lysodeikticus DNA were modified by exposure to reductively activated mitomycin C, an antitumor antibiotic. The resulting covalent drug-polynucleotide complexes displayed varying degrees of CD inversions, which are
Barton, Jacqueline K. +5 more
core +1 more source
The complete chloroplast genome of Aegle marmelos and its phylogenetic analysis
Mitochondrial DNA. Part B. Resources, 2023 Aegle marmelos (L.) Correa 1800, a plant belonging to the Rutaceae family, is extensively used in Tibetan medicine. We employed Illumina HiSeq reads to assemble the complete chloroplast (cp) genome of A. marmelos, which spans 144,538 bp.
Sijia Wang +6 more
doaj +1 more source
Potent CRISPR-Cas9 inhibitors from Staphylococcus genomes. [PDF]
, 2020 Anti-CRISPRs (Acrs) are small proteins that inhibit the RNA-guided DNA targeting activity of CRISPR-Cas enzymes. Encoded by bacteriophage and phage-derived bacterial genes, Acrs prevent CRISPR-mediated inhibition of phage infection and can also block ...
Doudna, Jennifer A +5 more
core +3 more sources
Next generation sequencing reveals the complete plastome sequence of newly discovered cliff-dwelling Sonchus boulosii (Asteraceae: Cichorieae) in Morocco [PDF]
, 2019 The complete chloroplast genome sequences of newly discovered cliff-dwelling species of Sonchus, S. boulosii, were reported in this study. The S. boulosii plastome was 152,016 bp long, with the large single copy (LSC) region of 83,988 bp, the small ...
Kim, Seon-Hee +2 more
core +1 more source
IUPACpal: efficient identification of inverted repeats in IUPAC-encoded DNA sequences
BMC Bioinformatics, 2021 Background An inverted repeat is a DNA sequence followed downstream by its reverse complement, potentially with a gap in the centre. Inverted repeats are found in both prokaryotic and eukaryotic genomes and they have been linked with countless possible ...
Hayam Alamro +4 more
doaj +1 more source
A Developmentally Regulated Deletion Element with Long Terminal Repeats Has \u3cem\u3eCis\u3c/em\u3e-Acting Sequences in the Flanking DNA [PDF]
, 2000 Approximately 6000 specific DNA deletion events occur during development of the somatic macronucleus of the ciliate Tetrahymena. The eliminated Tlr1 element is 13 kb or more in length and has an 825 bp inverted repeat near the rearrangement junctions.
Karrer, Kathleen M., Patil, Namrata S.
core +1 more source
A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins
FEBS Letters, EarlyView.We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue +6 more
wiley +1 more source
Comparative chloroplast genomes of photosynthetic orchids: insights into evolution of the Orchidaceae and development of molecular markers for phylogenetic applications. [PDF]
PLoS ONE, 2014 The orchid family Orchidaceae is one of the largest angiosperm families, including many species of important economic value. While chloroplast genomes are very informative for systematics and species identification, there is very limited information ...
Jing Luo +5 more
doaj +1 more source