Results 21 to 30 of about 26,663 (244)

New Insights in Thrombin Inhibition Structure–Activity Relationships by Characterization of Octadecasaccharides from Low Molecular Weight Heparin

Molecules, 2017
Low Molecular Weight Heparins (LMWH) are complex anticoagulant drugs that mainly inhibit the blood coagulation cascade through indirect interaction with antithrombin.
Pierre A. J. Mourier, Olivier Y. Guichard, Fréderic Herman, Philippe Sizun, Christian Viskov   +4 more
doaj   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Preparation and characterization of graphene oxide-based cation, chelating, and anion exchangers for salt removal

Heliyon
Due to the shortage of freshwater resources, and the limitations of commonly used ion exchangers, this study aims to prepare, characterize, and test new graphene-based ion exchangers for salt removal.
Mahmoud A. Al Azwani, Saleh Al Busafi, El-Said I. El-Shafey   +2 more
doaj   +1 more source

Preparation, Characterization, and In Vivo Evaluation of an Oral Multiple Nanoemulsive System for Co-Delivery of Pemetrexed and Quercetin

Pharmaceutics, 2018
Co-administration of conventional and natural chemotherapeutics offers synergistic anticancer efficacy while minimizing adverse effects. In this study, an oral co-delivery system for pemetrexed (PMX) and quercetin (QCN) was designed based on water-in-oil-
Rudra Pangeni, Vijay Kumar Panthi, In-Soo Yoon, Jin Woo Park   +3 more
doaj   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

Guanidinium Like‐Charge Ion Pairing and Oligoarginine Aggregation in Water by Nuclear Magnetic Resonance, Cryo‐Electron Microscopy, and Molecular Dynamics

Aggregate
Like‐charge pairing is a physical manifestation of the unique solvation properties of certain ion pairs in water. Water's high dielectric constant and related charge screening capability significantly influence the interaction between like‐charged ions ...
Denys Biriukov, Zuzana Osifová, Man Thi Hong Nguyen, Philip E. Mason, Martin Dračínský, Pavel Jungwirth, Jan Heyda, Mattia I. Morandi, Mario Vazdar   +8 more
doaj   +1 more source

Emerging experimental and computational methods for studying redox‐regulated structural transitions

FEBS Letters, EarlyView.
Redox reactions can reshape proteins and alter how they behave in cells, with important consequences for health and disease. This review explores emerging experimental and computational approaches for discovering these redox‐sensitive protein switches, revealing their structural effects, and predicting their behavior, opening new opportunities to ...
Tasneem Rass, Dana Reichmann, Gábor Erdős   +2 more
wiley   +1 more source

The Effects of Counter-Ions on Peptide Structure, Activity, and Applications

Biomolecules
Peptide drug development has emerged as a prominent area in pharmaceutical research due to its high specificity and therapeutic potential. However, their biological activity, stability, and bioavailability are significantly influenced by interactions ...
Ying Liu, Yi Huang, Lan Yang, Yu Gao, Zheng Jia, Tingting Liu, Baoling Su, Chuyuan Wang, Lili Jin, Dianbao Zhang   +9 more
doaj   +1 more source

Structural and biochemical analysis of a B12 superbinder

FEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices, Jacob J. Whittaker, Dirk J. Slotboom, Albert Guskov   +3 more
wiley   +1 more source

Structures of mycobacterial 3‐methylcrotonyl‐CoA carboxylase reveal carrier‐domain translocation between catalytic sites

FEBS Letters, EarlyView.
Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav, Bogdan I. Florea, Sebastian Geibel   +2 more
wiley   +1 more source