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SaSi qPCR: a novel duplex qPCR assay for rapid and simultaneous detection of Streptococcus agalactiae and Streptococcus iniae in fish. [PDF]
Taengphu S +3 more
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Genome Characterization and Phylogenetic Analysis of Scale Drop Disease Virus Isolated from Asian Seabass (Lates calcarifer). [PDF]
Chokmangmeepisarn P +7 more
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Identification of infectious spleen and kidney necrosis virus (ISKNV)-encoded microRNAs
Virus Genes, 2020MicroRNAs (miRNAs) are small noncoding RNAs that post-transcriptionally regulate gene expression by complementary binding to target mRNAs. Virus-encoded miRNAs play important roles in virus life cycle and virus-host interactions. Viruses from the Megalocytivirus genus, family Iridoviridae, infect a wide range of fishes, bringing great challenges to ...
Jian-hui He +4 more
openaire +2 more sources
Diseases of Aquatic Organisms, 2020
Using cultures of the SKF-9 cell line, megalocytivirus AFIV-16 was isolated from imported angelfish Pterophyllum scalare held in quarantine at the Australian border. The cytopathic effect caused by isolate AFIV-16 presented as cell rounding and enlargement, but complete destruction of the infected cell cultures did not occur.
Yasuhiko, Kawato +10 more
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Using cultures of the SKF-9 cell line, megalocytivirus AFIV-16 was isolated from imported angelfish Pterophyllum scalare held in quarantine at the Australian border. The cytopathic effect caused by isolate AFIV-16 presented as cell rounding and enlargement, but complete destruction of the infected cell cultures did not occur.
Yasuhiko, Kawato +10 more
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Identification of intron in ORF003 gene and its application for inactivation test of ISKNV
Microbial Pathogenesis, 2020The virus inactivation test is a critical skill in inactivated vaccine production. Active viruses produced viral mRNA in susceptible cells or the host can be used to infer whether a DNA virus is replicating by RT-PCR. But it is generally difficult to avoid genomic DNA contamination in the samples.
Xiaozhe, Fu +7 more
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Aquaculture, 2021
Abstract The spotted knifejaw (Oplegnathus punctatus) is an emerging economical aquaculture species in China in recent years. In the present study, we characterized an iridovirus-like disease outbreak in spotted knifejaw aquaculture industry in Shandong Province (SKIV-SD), China.
Xiaohong Huang +8 more
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Abstract The spotted knifejaw (Oplegnathus punctatus) is an emerging economical aquaculture species in China in recent years. In the present study, we characterized an iridovirus-like disease outbreak in spotted knifejaw aquaculture industry in Shandong Province (SKIV-SD), China.
Xiaohong Huang +8 more
openaire +1 more source
Administration of recombinant IFN1 protects zebrafish (Danio rerio) from ISKNV infection
Fish & Shellfish Immunology, 2010In the present study, we used the infectious spleen and kidney necrosis virus (ISKNV) and zebrafish model system to investigate the inhibitory effect of recombinant zebrafish interferon 1 (zfrIFN1) on acute viral infection and the impact of time of zfrIFN1 administration on its protective efficacy.
Zhongsheng, Li +9 more
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Fish & Shellfish Immunology, 2022
Infectious spleen and kidney necrosis virus (ISKNV), the type species of the genus Megalocytivirus, infects a variety of teleost fish species and causes substantial losses in the aquaculture industry worldwide. ISKNV ORF71L is 1611 bp in length, encodes a 537-amino-acid peptide and was previously identified as a viral structural protein in the ISKNV ...
Hetong Zhang +4 more
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Infectious spleen and kidney necrosis virus (ISKNV), the type species of the genus Megalocytivirus, infects a variety of teleost fish species and causes substantial losses in the aquaculture industry worldwide. ISKNV ORF71L is 1611 bp in length, encodes a 537-amino-acid peptide and was previously identified as a viral structural protein in the ISKNV ...
Hetong Zhang +4 more
openaire +2 more sources

