Results 111 to 120 of about 493 (138)

Identification of intron in ORF003 gene and its application for inactivation test of ISKNV

Microbial Pathogenesis, 2020
The virus inactivation test is a critical skill in inactivated vaccine production. Active viruses produced viral mRNA in susceptible cells or the host can be used to infer whether a DNA virus is replicating by RT-PCR. But it is generally difficult to avoid genomic DNA contamination in the samples.
Xiaozhe, Fu, Lixi, Zhang, Lihui, Liu, Qiang, Lin, Hongru, Liang, Yinjie, Niu, Zhibin, Huang, Ningqiu, Li   +7 more
openaire   +2 more sources

Identification of infectious spleen and kidney necrosis virus (ISKNV)-encoded microRNAs

Virus Genes, 2020
MicroRNAs (miRNAs) are small noncoding RNAs that post-transcriptionally regulate gene expression by complementary binding to target mRNAs. Virus-encoded miRNAs play important roles in virus life cycle and virus-host interactions. Viruses from the Megalocytivirus genus, family Iridoviridae, infect a wide range of fishes, bringing great challenges to ...
Jian-hui He, Qiong Xia, Shaoping Weng, Jianguo He, Xiaopeng Xu   +4 more
openaire   +2 more sources

Transcriptional programs of infectious spleen and kidney necrosis virus (ISKNV) in vitro and in vivo

Virus Genes, 2020
Infectious spleen and kidney necrosis virus (ISKNV), causing serious infectious diseases to marine and freshwater fishes, is the type species of the genus Megalocytivirus, family Iridoviridae. In this study, the transcriptional programs of ISKNV in vitro (MFF-1 cells) and in vivo (spleens from mandarin fish) were investigated using real-time PCR ...
Jian-hui He, Lichao Huang, Zhixun Guo, Shaoping Weng, Jianguo He, Xiaopeng Xu   +5 more
openaire   +2 more sources

Isolation, identification and genomic analysis of an ISKNV-type megalocytivirus from spotted knifejaw (Oplegnathus punctatus)

Aquaculture, 2021
Abstract The spotted knifejaw (Oplegnathus punctatus) is an emerging economical aquaculture species in China in recent years. In the present study, we characterized an iridovirus-like disease outbreak in spotted knifejaw aquaculture industry in Shandong Province (SKIV-SD), China.
Xiaohong Huang, Jingguang Wei, Qi Zheng, Ya Zhang, Weibin Zhu, Jiangchun Liu, Yunxia Hou, Qiwei Qin, Youhua Huang   +8 more
openaire   +1 more source

Transcriptomic and proteomic analyses of the immune mechanism in pathogenetic and resistant mandarin fish (Siniperca chuatsi) infected with ISKNV

Aquaculture, 2021
Abstract Mandarin fish (Siniperca chuatsi) is one of the most economically important aquaculture species in China. Mandarin fish aquaculture has experienced major losses in the past few decades because of outbreaks of infectious spleen and kidney necrosis virus (ISKNV). Little is known about the immune mechanism of pathogenetic and resistant mandarin
Hezhong Huang
exaly   +2 more sources

Display of ISKNV orf086 protein on the surface of Aeromonas hydrophila and its immunogenicity in Chinese perch ( Siniperca chuatsi )

Fish and Shellfish Immunology, 2016
Co-infection with infectious spleen and kidney necrosis virus (ISKNV) and Aeromonas hydrophila is becoming ever more widespread in Chinese perch (Siniperca chuatsi) aquaculture industry, so that it's necessary to develop the combined vaccine against ISKNV and A. hydrophila disease.
Qiang Lin, Ningqiu Li, Xiaozhe Fu
exaly   +3 more sources

First report of the infectious spleen and kidney necrosis virus (ISKNV) infection in ornamental fishes in India

Transboundary and Emerging Diseases, 2020
Infectious spleen and kidney necrosis virus (ISKNV), a member of family iridoviridae, reported for the first time in a wide range of ornamental fish species in India. Significant mortalities during the year 2018-19 were reported from a number of retailers in the region with various clinical signs.
S. K. Girisha, K. B. Kushala, M. S. Nithin, T. G. Puneeth, B. T. Naveen Kumar, T. N. Vinay, T. Suresh, S. K. Ajay, M. N. Venugopal, K. S. Ramesh   +9 more
openaire   +2 more sources

Molecular epidemiology of Infectious Spleen and Kidney Necrosis Virus (ISKNV) in Ghanaian cultured tilapia

2022
1 ABSTRACT Infectious Spleen and Kidney Necrosis Virus (ISKNV) is increasingly gaining more attention globally, due to its highly significant economic impact on the aquaculture industry. In late 2018, unusually high levels of mortality (60-90%) was reported in some intensive tilapia cage culture systems in Ghana ...
Angela Naa Amerley Ayiku, Abigail Akosua Adelani, Patrick Appenteng, Mary Nkansah, Joyce M. Ngoi, Collins Misita Morang’a, Richard Paley, Kofitsyo S. Cudjoe, David Verner-Jeffreys, Peter Kojo Quashie, Samuel Duodu   +10 more
openaire   +1 more source

A recombinant baculovirus vector vaccine (BacMCP) against the infectious spleen and kidney necrosis virus (ISKNV)

Journal of Fish Diseases, 2022
AbstractThe infectious spleen and kidney necrosis virus (ISKNV) is a highly lethal virus, which has brought significant losses to aquaculture. Therefore, a new vaccine against ISKNV with high efficiency, safety and convenience must be developed. While baculoviruses are more commonly used as protein expression systems for vaccine antigen production ...
Min Zhu, Zeen Shen, Yuchao Gu, Xinyu Tong, Yaxin Zhang, Jun Pan, Yongjie Feng, Xiaolong Hu, Yujun Wang, Guangli Cao, Renyu Xue, Chengliang Gong   +11 more
openaire   +2 more sources

Characterization of a membrane protein (VP001L) from infectious spleen and kidney necrosis virus (ISKNV)

Virus Genes, 2007
Infectious spleen and kidney necrosis virus (ISKNV) is the type species of megalocytivirus, Iridoviridae. A novel membrane protein corresponding to the first open reading frame (ORF001L) of ISKNV genome was identified. This 378-residue protein, termed the VP001L protein, has a high content of hydrophobic sequences and contains 10-11 putative ...
Xiaopeng, Xu, Jing, Lu, Qingxia, Lu, Hui, Zhong, Shaoping, Weng, Jianguo, He   +5 more
openaire   +2 more sources