Results 61 to 70 of about 493 (138)

The Future of Pathogen Detection in Aquaculture: Miniature Labs, Field‐Compatible Assays, environmental DNA and RNA, CRISPR and Metatranscriptomics

Aquaculture, Fish and Fisheries, Volume 5, Issue 3, June 2025.
ABSTRACT Inland recirculating aquaculture is a thriving food industry providing sustainable and locally sourced high‐quality protein. However, its expansion is accompanied by emerging challenges regarding the spread of pathogens and diseases. Detection and management of pathogens in aquaculture remain underdeveloped compared to other animal farming ...
Jessica Rieder, Anastasiia Berezenko, Alexandra Meziti, Irene Adrian‐Kalchhauser   +3 more
wiley   +1 more source

Megalocytivirus: A Review of Epidemiology, Pathogenicity, Immune Evasion, and Prevention Strategies

Reviews in Aquaculture, Volume 17, Issue 3, June 2025.
ABSTRACT Megalocytivirus, a large double‐stranded DNA virus belonging to the Iridoviridae family, has infected over 100 species of fish, leading to significant economic losses in the aquaculture, food, and ornamental fish industries. These viruses exhibit icosahedral symmetry and have diameters ranging from 120 to 200 nm.
Changjun Guo, Jian He, Xiaopeng Xu, Shaoping Weng, Jianguo He   +4 more
wiley   +1 more source

State‐of‐the‐art in food safety: The potential of CRISPR/Cas‐based lateral flow assay technology in the field of food analysis

Food Safety and Health, Volume 3, Issue 2, Page 155-171, April 2025.
The CRISPR‐LFA merges the CRISPR/Cas system with a LFA strip. This approach utilizes the programmability and specificity of the CRISPR/Cas system for detecting various nucleic acid molecules. Abstract Food safety is essential for public health, with key hazards including microbial and chemical contamination and pesticide residues.
Yang Sun, Kunli Zhong, Lanmengya Nie, Yan Xu, Yiran Wang, Jingtao Yuan, Shuai Jiang, Xu Li, Zhidong Zhang, Xiangfei Li, Ling Jiang   +10 more
wiley   +1 more source

Investigation of Tilapia (Oreochromis niloticus) Mortality Events Targeting Tilapia Lake Virus Disease (TiLVD) in Bangladesh

Veterinary Medicine and Science, Volume 11, Issue 2, March 2025.
Investigation of unusual tilapia mortalities in Bangladesh targeting Tilapia lake virus disease (TiLVD) was done using combined classical (case study and histopathology) and molecular (PCR/real‐time PCR) approaches. No evidence of Tilapia lake virus (TiLV) was detected, highlighting the need for broader studies to uncover alternative causes.
Mst. Sabrina Akter, Nusrat Jahan Punom, Md. Mostavi Enan Eshik, Shawon Ahmmed, Md. Golam Rabbane, Mohammad Shamsur Rahman   +5 more
wiley   +1 more source

Fish Genomics and Its Application in Disease‐Resistance Breeding

Reviews in Aquaculture, Volume 17, Issue 1, January 2025.
ABSTRACT Global aquaculture production has been rising for several decades, with up to 76% of the total production from fish. However, the problem of fish diseases is becoming more and more prominent in today's context of pursuing sustainable aquaculture.
Yu Huang, Zeyu Li, Mengcheng Li, Xinhui Zhang, Qiong Shi, Zhen Xu   +5 more
wiley   +1 more source

Distribution and Risk Factors Associated With Tilapia Parvovirus (TiPV) Presence in Red Hybrid Tilapia (Oreochromis spp.) Farms in Thailand

Transboundary and Emerging Diseases, Volume 2025, Issue 1, 2025.
Emerging viral diseases, such as tilapia parvovirus (TiPV), are having a significant economic impact on global tilapia aquaculture. TiPV is responsible for the mass mortality of Nile tilapia (Oreochromis niloticus) and red hybrid tilapia (Oreochromis spp.) in China, India and Thailand.
Benya Chatkaewchai, Win Surachetpong, Suporn Thongyuan, Methanan Kamklang, Sudtisa Laopiem, Sakuna Pattanakunanan, Visanu Boonyawiwat, Theeraporn Pulpipat, Fedor Korennoy   +8 more
wiley   +1 more source

PCR Detection and Phylogenetic Analysis of Megalocytivirus Isolates in Farmed Giant Sea Perch Lates calcarifer in Southern Taiwan

Viruses, 2020
The Megalocytivirus genus includes three genotypes, red sea bream iridovirus (RSIV), infectious spleen and kidney necrosis virus (ISKNV), and turbot reddish body iridovirus (TRBIV), and has caused mass mortalities in various marine and freshwater fish ...
Jia-Ming Tsai, Song-Lang Huang, Chung-Da Yang   +2 more
doaj   +1 more source

Monogenean Sparicotyle chrysophrii: The major pathogen of the Mediterranean gilthead seabream aquaculture

Reviews in Aquaculture, Volume 16, Issue 1, Page 287-308, January 2024.
Sparicotylosis has been present in Mediterranean aquaculture of the gilthead seabream for the past 25 years, yet disease management and treatments are not effective. This is further jeopardised by global warming and resistance of Sparicotyle chrysophrii to available anthelminthic compounds.
Ivona Mladineo, Donatella Volpatti, Paola Beraldo, George Rigos, Pantelis Katharios, Francesc Padros   +5 more
wiley   +1 more source

Virus Overlay Assay (Far-Western blotting)

Bio-Protocol, 2013
Virus overlay assay is a method to detect protein-protein interaction in vitro. We performed the virus overlay assay to identify the receptor proteins interacting with the infectious spleen and kidney necrosis virus (ISKNV).
Kun-Tong Jia, Chang-Jun Guo, Xiao-Bo Yang, Jian-Guo He   +3 more
doaj   +1 more source

Structure-Based Screening of Antiviral Candidates Against Infectious Spleen and Kidney Necrosis Virus (ISKNV) Using Molecular Docking and In Vitro Evaluation

Biomolecules
Infectious spleen and kidney necrosis virus (ISKNV) causes systemic infections and high mortality in various freshwater and marine fish species, posing a serious threat to aquaculture and ornamental fish industries.
Eun-Jin Baek, Hyun-Deok Choi, Ye-Jin Jeong, Kwang-Il Kim   +3 more
doaj   +1 more source