Results 31 to 40 of about 120,438 (289)

Multidimensional separation prior to mass spectrometry: Getting closer to the bottom of the iceberg [PDF]

, 2013
While prefractionation has previously been shown to improve results in MS analysis, a novel combination provides an additional dimension of separation: protein fractionation by SDS-PAGE followed by IEF of tryptic peptides before separation by RP-LC ...
Mayr, Manuel, Rabilloud, Thierry
core   +4 more sources

Defining the right diluent for intravenous infusion of therapeutic antibodies

mAbs, 2020
Therapeutic monoclonal antibodies (mAbs) are commonly administered to patients through intravenous (IV) infusion, which involves diluting the medication into an infusion solution (e.g., saline and 5% dextrose).
Shen Luo, Keisha Melodi McSweeney, Tao Wang, Silvia M. Bacot, Gerald M. Feldman, Baolin Zhang   +5 more
doaj   +1 more source

Finger printing human norovirus-like particles by capillary isoelectric focusing with whole column imaging detection

Virus Research, 2022
Owing to the limitation of in vitro culture of human noroviruses (HuNoVs), the development of HuNoV vaccines has to depend on the self-assembling virus-like particles (VLPs) with capsid protein expression.
Jialiang Du, Gang Wu, Chunbo Cui, Chuanfei Yu, Yongfei Cui, Luyun Guo, Yueyue Liu, Yan Liu, Wenbo Wang, Chunyu Liu, Zhihao Fu, Meng Li, Sha Guo, Xiaojuan Yu, Yalan Yang, Maoqin Duan, Gangling Xu, Lan Wang   +17 more
doaj   +1 more source

Evidence for the adaptation of protein pH-dependence to subcellular pH

BMC Biology, 2009
Background The availability of genome sequences, and inferred protein coding genes, has led to several proteome-wide studies of isoelectric points. Generally, isoelectric points are distributed following variations on a biomodal theme that originates ...
Warwicker Jim, Chan Pedro
doaj   +1 more source

Sensitivity and specificity of detection methods for erythropoietin doping in cyclists [PDF]

, 2019
Recombinant human erythropoietin (rHuEPO) is used as doping a substance. Anti-doping efforts include urine and blood testing and monitoring the athlete biological passport (ABP).
Burggraaf, Jacobus, Cohen, Adam F, Daniels, Johannes MA, Gal, Pim, Heuberger, Jules AAC, Hon, Olivier, Post, Titiaan E, Ram, Herman, Rotmans, Joris I, Stuurman, Frederik E, Van Eenoo, Peter   +10 more
core   +1 more source

Quantification of apoC-II and apoC-III of human very low density lipoproteins by analytical isoelectric focusing.

Journal of Lipid Research, 1978
ApoC-II and apoC-III of human very low density lipoproteins (VLDL) have been quantified by analytical isoelectric focusing (IEF) between pH 4 and 6 in polyacrylamide gels containing 8 M urea.
A L Catapano, R L Jackson, E B Gilliam, A M Gotto, Jr, L C Smith   +4 more
doaj   +1 more source

Electrophoretic separation of proteins via complexation with a polyelectrolyte

, 2002
We suggest to augment standard isoelectronic focusing for separation of proteins in a gradient of pH by a similar focusing in the presence of a strongly charged polyelectrolyte (PE).
B.I. Shklovskii, Blatter, E.M. Baskin, G.V. Zilberstein, Grosberg, Kabanov, Netz, Nguyen, Rabilloud, Righetti, Righetti, Shklovskii, Shklovskii   +12 more
core   +1 more source

Quantitative Preparative Native Continuous Polyacrylamide Gel Electrophoresis (QPNC-PAGE) [PDF]

, 2008
QPNC-PAGE, or quantitative preparative native continuous polyacrylamide gel electrophoresis, is a high-resolution technique applied in biochemistry and bioinorganic chemistry to separate proteins by isoelectric point.
Bernd Kastenholz
core   +2 more sources

Optimizing the Extraction of Protein from Defatted Schizochytrium Cell Residues and Studying the Emulsification Characteristics of Protein

Fermentation
In this study, proteins were prepared from Schizochytrium pombe residue after oil extraction using isoelectric point precipitation, and their physicochemical and emulsifying properties were investigated.
Yingying Yang, Xiangying Zhao, Liping Liu, Xinyu Wang, Ruiguo Li, Jiaxiang Zhang   +5 more
doaj   +1 more source

Evaluation of baseline ECG in patients undergoing Oral Flecainide Challenge test for suspected Brugada Syndrome: An analysis of lead II

Indian Pacing and Electrophysiology Journal, 2017
Background and Objectives: We analyzed Lead II in patients undergoing an Oral Flecainide Challenge test (FCT), to identify any pointers that could predict a positive FCT and thereby help in recognition of latent BS.
Maneesh K. Rai, MD, DM, Mukund A. Prabhu, MD, DM, Jayaprakash Shenthar, MD, DM, FACC, Natarajan Kumaraswamy U, MD, DM, Ritesh Vekariya, MD, Padmanabh Kamath, MD, DM, Narasimha Pai, MD, DM, FACC, Ramanath L. Kamath, MD, DM, Vivek Pillai, MD, DM   +8 more
doaj   +1 more source