Results 1 to 10 of about 2,507,233 (216)

Outer Membrane Vesicle Induction and Isolation for Vaccine Development

Frontiers in Microbiology, 2021
Gram-negative bacteria release vesicular structures from their outer membrane, so called outer membrane vesicles (OMVs). OMVs have a variety of functions such as waste disposal, communication, and antigen or toxin delivery.
Melanie D. Balhuizen, Edwin J. A. Veldhuizen, Henk P. Haagsman   +2 more
doaj   +3 more sources

Towards a membrane proteome in Drosophila: a method for the isolation of plasma membrane [PDF]

BMC Genomics, 2010
Background The plasma membrane (PM) is a compartment of significant interest because cell surface proteins influence the way in which a cell interacts with its neighbours and its extracellular environment.
Thomas Graham H, Stanley Bruce A, Khanna Mansi R   +2 more
doaj   +3 more sources

Detergent-Free Isolation of Membrane Proteins and Strategies to Study Them in a Near-Native Membrane Environment

Biomolecules, 2022
Atomic-resolution structural studies of membrane-associated proteins and peptides in a membrane environment are important to fully understand their biological function and the roles played by them in the pathology of many diseases.
Bankala Krishnarjuna, Ayyalusamy Ramamoorthy   +1 more
doaj   +3 more sources

iEVIP: a dual-inspired intelligent platform for smart response extracellular vesicle isolation and single-vesicle profiling [PDF]

Nature Communications
Extracellular vesicles (EVs) are promising non-invasive biomarkers for early cancer detection, yet clinical translation is limited by membrane fouling during isolation and vesicle aggregation during single-vesicle imaging.
Han Xie, Tucan Chen, Peiyu Yan, Yuqi Fu, Mengcheng Lei, Yuanyuan Liu, Xudong Zhao, Wei Du, Xiaojun Feng, Xin Liu, Yiwei Li, Peng Chen, Bi-Feng Liu   +12 more
doaj   +2 more sources

Morphology of Phagophore Precursors by Correlative Light-Electron Microscopy

Cells, 2022
Autophagosome biogenesis occurs in the transient subdomains of the endoplasmic reticulum that are called omegasomes, which, in fluorescence microscopy, appear as small puncta, which then grow in diameter and finally shrink and disappear once the ...
Sigurdur Runar Gudmundsson, Katri A. Kallio, Helena Vihinen, Eija Jokitalo, Nicholas Ktistakis, Eeva-Liisa Eskelinen   +5 more
doaj   +1 more source

Key Regulators of Autophagosome Closure

Cells, 2021
Autophagy is an evolutionarily conserved pathway, in which cytoplasmic components are sequestered within double-membrane vesicles called autophagosomes and then transported into lysosomes or vacuoles for degradation.
Wenyan Jiang, Xuechai Chen, Cuicui Ji, Wenting Zhang, Jianing Song, Jie Li, Juan Wang   +6 more
doaj   +1 more source

The Role of PDE11A4 in Social Isolation-Induced Changes in Intracellular Signaling and Neuroinflammation

Frontiers in Pharmacology, 2021
Phosphodiesterase 11A (PDE11A), an enzyme that degrades cyclic nucleotides (cAMP and cGMP), is the only PDE whose mRNA expression in brain is restricted to the hippocampal formation.
Katy Pilarzyk, Reagan Farmer, Latarsha Porcher, Michy P. Kelly, Michy P. Kelly, Michy P. Kelly   +5 more
doaj   +1 more source

Improved volume CLEM revealed that aberrant phagophores and RB1CC1/FIP200-containing clusters appear surround SQSTM1/p62 aggregates in Atg9a-deficient cells

Autophagy Reports, 2023
ATG9A is an important membrane protein in mammalian macroautophagy. The formation of autophagosomes and phagophores is blocked in atg9a KO cells. However, it remains possible that residual membrane formation activity exists in these cells.
Soichiro Kakuta, Junji Yamaguchi, Chigure Suzuki, Isei Tanida, Yasuo Uchiyama   +4 more
doaj   +1 more source

A mechanism that ensures non-selective cytoplasm degradation by autophagy

Nature Communications, 2023
In autophagy, a membrane cisterna called the isolation membrane expands, bends, becomes spherical, and closes to sequester cytoplasmic constituents into the resulting double-membrane vesicle autophagosome for lysosomal/vacuolar degradation.
Tetsuya Kotani, Yuji Sakai, Hiromi Kirisako, Chika Kakuta, Soichiro Kakuta, Yoshinori Ohsumi, Hitoshi Nakatogawa   +6 more
doaj   +1 more source

Casein Kinase 1 Family Member CK1δ/Hrr25 Is Required for Autophagosome Completion

Frontiers in Cell and Developmental Biology, 2020
Autophagy starts with the initiation and nucleation of isolation membranes, which further expand and seal to form autophagosomes. The regulation of isolation membrane closure remains poorly understood.
Yuting Li, Xuechai Chen, Qianqian Xiong, Yong Chen, Hongyu Zhao, Muhammad Tahir, Jingdong Song, Bing Zhou, Juan Wang   +8 more
doaj   +1 more source