Results 31 to 40 of about 1,162,423 (312)

Modular detergents tailor the purification and structural analysis of membrane proteins including G-protein coupled receptors [PDF]

, 2020
Detergents enable the purification of membrane proteins and are indispensable reagents instructural biology. Even though a large variety of detergents have been developed in the lastcentury, the challenge remains to identify guidelines that allowfine ...
Almeida, F., Bolla, J., Ehrmann, S., Gault, J., Haag, R., Hoi, K., Liko, I., Pagel, K., Robinson, C., Schweder, M., Shutin, D., Urner, L., Yen, H.   +12 more
core   +2 more sources

Purification of Protein-complexes from the Cyanobacterium Synechocystis sp. PCC 6803 Using FLAG-affinity Chromatography

Bio-Protocol, 2020
Exploring the structure and function of protein complexes requires their isolation in the native state–a task that is made challenging when studying labile and/or low abundant complexes.
Minna Koskela, Petra Skotnicová, Éva Kiss, Roman Sobotka   +3 more
doaj   +1 more source

Mammalian Cell-derived Vesicles for the Isolation of Organelle Specific Transmembrane Proteins to Conduct Single Molecule Studies

Bio-Protocol, 2018
Cell-derived vesicles facilitate the isolation of transmembrane proteins in their physiological membrane maintaining their structural and functional integrity.
Faruk Moonschi, Ashley Fox-Loe, Xu Fu, Christopher Richards   +3 more
doaj   +1 more source

Optimization of gradual hemolysis for isolation of hemoglobin from bovine erythrocytes [PDF]

Hemijska Industrija, 2012
In this work, we describe an optimized procedure based on gradual hemolysis for the isolation of hemoglobin derived from bovine slaughterhouse erythrocytes in a membrane bioreactor.
Pravilović Radoslava, Mojsilović Slavko, Kostić Ivana, Ilić Vesna, Bugarski Diana, Đorđević Verica, Bugarski Branko   +6 more
doaj   +1 more source

Microsomal membrane isolation v1

, 2023
Microsomal membrane isolation
Rania Abou El Asrar, Peter Vangheluwe
openaire   +1 more source

Comparative studies of Yersinia pestis outer membrane isolation techniques and their potential use in plague epidemiology Estudo comparativo de técnicas de isolamento de membrana externa de Yersinia pestis e seu uso na epidemiologia da peste

Revista do Instituto de Medicina Tropical de São Paulo, 1990
In the present study three techniques for obtaining outer membrane enriched fractions from Yersinia pestis were evaluated. The techniques analysed were: differential solubilization of the cytoplasmic membrane with Sarkosyl or Triton X-100, and ...
Frederico Guilherme Coutinho Abath, Luís Carlos de Sousa Ferreira   +1 more
doaj   +1 more source

Revealing the structure of land plant photosystem II: the journey from negative‐stain EM to cryo‐EM

FEBS Letters, EarlyView.
Advances in cryo‐EM have revealed the detailed structure of Photosystem II, a key protein complex driving photosynthesis. This review traces the journey from early low‐resolution images to high‐resolution models, highlighting how these discoveries deepen our understanding of light harvesting and energy conversion in plants.
Roman Kouřil
wiley   +1 more source

Isolation and Differentiation of Amniotic Membrane Stem Cells Into Keratinocytes

Cell Transplantation, 2020
The human amniotic membrane is a highly abundant and readily available tissue that may be useful for regenerative medicine and cell therapy. The amniotic membrane stem cells can differentiate into multiple cell lineages; they have low immunogenicity and ...
Dam Thi Phuong Lan, Pham Thai Binh, Nguyen Thi Quynh Giang, Can Van Mao, Dang Thanh Chung, Nong Van Diep, Do Minh Trung, Pham Van Tran   +7 more
doaj   +1 more source

Comparative Analysis of Outer Membrane Vesicle Isolation Methods With an Escherichia coli tolA Mutant Reveals a Hypervesiculating Phenotype With Outer-Inner Membrane Vesicle Content

Frontiers in Microbiology, 2021
Outer membrane vesicles (OMVs) produced by Gram-negative bacteria are mediators of cell survival and pathogenesis by facilitating virulence factor dissemination and resistance to antimicrobials.
Shelby L. Reimer, Daniel R. Beniac, Shannon L. Hiebert, Timothy F. Booth, Patrick M. Chong, Garrett R. Westmacott, George G. Zhanel, Denice C. Bay   +7 more
doaj   +1 more source

Using eggshell membranes as a DNA source for population genetic research [PDF]

, 2009
In the context of population genetic research, a faster and less invasive method of DNA sampling would allow large-scale assessments of genetic diversity and genetic differentiation with the help of volunteer observers.
Broekman, J., Kentie, R., Musters, C.J.M., Snoo, G.R., de, Trimbos, K.B.   +4 more
core   +3 more sources