Results 31 to 40 of about 2,507,233 (216)
iMEM: Isolation of Plasma Membrane for Cryoelectron Microscopy. [PDF]
, 2016 The plasma membrane and the cell cortex are essential parts of the eukaryotic cell. The plasma membrane delimitates the cell and mediates communication with the outside.
Beckmann, Sven +2 more
core +1 more source
Physiological Reports, 2020 Intact cardiomyocytes are used to investigate cardiac contractility and evaluate the efficacy of new therapeutic compounds. Primary enzymatic isolation of adult rodent cardiomyocytes has limitations, including low cardiomyocyte survival, which is likely ...
Teresa L. Czeiszperger +2 more
doaj +1 more source
Factors influencing the solubilization of membrane proteins from Escherichia coli membranes by styrene–maleic acid copolymers [PDF]
, 2020 Styrene–maleic acid (SMA) copolymers are a promising alternative to detergents for the solubilization of membrane proteins. Here we employ Escherichia coli membranes containing KcsA as a model protein to investigate the influence of different ...
Dörr, Jonas M. +7 more
core +1 more source
A simplified method for the preparation of detergent-free lipid rafts
Journal of Lipid Research, 2005 Lipid rafts are small plasma membrane domains that contain high levels of cholesterol and sphingolipids. Traditional methods for the biochemical isolation of lipid rafts involve the extraction of cells with nonionic detergents followed by the separation ...
Jennifer L. Macdonald, Linda J. Pike
doaj +1 more source
Bio-Protocol, 2020 Exploring the structure and function of protein complexes requires their isolation in the native state–a task that is made challenging when studying labile and/or low abundant complexes.
Minna Koskela +3 more
doaj +1 more source
Isolation and immunofluorescence staining of Aspergillus fumigatus conidia-containing phagolysosomes
STAR Protocols, 2021 Summary: The analysis of phagolysosomes within professional phagocytic cells is facilitated by their isolation. Here, we optimized a protocol for the isolation of intact phagolysosomes from macrophages infected with the spores of Aspergillus fumigatus ...
Marie Goldmann +4 more
doaj +1 more source
Fusion of Isolated Sarcoplasmic Reticulum Membranes
Zeitschrift für Naturforschung C, 1976 Abstract Fragmented sarcoplasmic reticulum (FSR) vesicles from rabbit muscle were suspended in 1.5 - 5% glycerol solutions and were pelleted onto aluminum foil disks in a modified centrifuge tube. Examination of these pellets in the electron microscope after drying for 2 - 2.5, 4 - 5.5, and 21 hours revealed a progression of changes ...
Stromer , M., Hasselbach, W.
openaire +3 more sources
Membrane fouling in osmotically driven membrane processes: A review
, 2016 The utilization of osmosis for engineered applications sparked off various emerging technologies relying on osmotically driven membrane processes (ODMPs).
Fane, Anthony Gordon +7 more
core +1 more source
Bio-Protocol, 2018 Cell-derived vesicles facilitate the isolation of transmembrane proteins in their physiological membrane maintaining their structural and functional integrity.
Faruk Moonschi +3 more
doaj +1 more source
Revista do Instituto de Medicina Tropical de São Paulo, 1990 In the present study three techniques for obtaining outer membrane enriched fractions from Yersinia pestis were evaluated. The techniques analysed were: differential solubilization of the cytoplasmic membrane with Sarkosyl or Triton X-100, and ...
Frederico Guilherme Coutinho Abath +1 more
doaj +1 more source