Results 51 to 60 of about 1,162,423 (312)

Sites of Biosynthesis of Outer and Inner Membrane Proteins of Neurospora crassa Mitochondria [PDF]

, 1970
Outer and inner membranes of Neurospora crassa mitochondria were separated by the combined swelling, shrinking, sonication procedure. Membranes were characterized by electron microscopy and by marker enzyme activities.
Aasen A. J., Arrigoni O., Beattie D. S., Beattie D. S., Beisenherz G., Cassady W. E., Goodwin T. W., Harding R. W., Hawley E. S., Neupert W., Parsons D., Schnaitman G., Schott H.-H., Vogel H. J., Weiss H.   +14 more
core   +1 more source

An upstream open reading frame regulates expression of the mitochondrial protein Slm35 and mitophagy flux

FEBS Letters, EarlyView.
This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva, Ariann E. Mendoza‐Martínez, P. Abril Medina‐Flores, Carlos Campero‐Basaldua, Alexander DeLuna, Soledad Funes   +5 more
wiley   +1 more source

Characterization of a monoclonal antibody that induces the acrosome reaction of sea urchin sperm. [PDF]

, 1987
A monoclonal antibody, J18/29, induces the acrosome reaction (AR) in spermatozoa of the sea urchin Strongylocentrotus purpuratus. J18/29 induces increases in both intracellular Ca2+ and intracellular pH similar to those occurring upon induction of the AR
Ebina, Y, Meinhof, CG, Schackmann, RW, Trimmer, JS, Vacquier, VD   +4 more
core  

In situ molecular organization and heterogeneity of the Legionella Dot/Icm T4SS

FEBS Letters, EarlyView.
We present a nearly complete in situ model of the Legionella Dot/Icm type IV secretion system, revealing its central secretion channel and identifying new components. Using cryo‐electron tomography with AI‐based modeling, our work highlights the structure, variability, and mechanism of this complex nanomachine, advancing understanding of bacterial ...
Przemysław Dutka, Yuxi Liu, Stefano Maggi, Debnath Ghosal, Jue Wang, Prashant P. Damke, Stephen D. Carter, Wei Zhao, Sukhithasri Vijayrajratnam, Joseph P. Vogel, Carrie L. Shaffer, Grant J. Jensen   +11 more
wiley   +1 more source

Membrane Vesicles as Drug Delivery Systems: Source, Preparation, Modification, Drug Loading, In Vivo Administration and Biodistribution, and Application in Various Diseases

Pharmaceutics, 2023
Bioinspired (or biologically inspired) drug delivery systems (DDSs) have been intensively studied in the last decades. As bioinspired DDSs, membrane vesicles, including extracellular vesicles (EVs) released from eukaryotic cells, outer membrane vesicles (
Chenhan Sun, Ying Qin, Hongda Zhuang, Yuan Zhang, Zhiwen Wu, Yong Chen   +5 more
doaj   +1 more source

Resuscitation and quantification of stressed Escherichia coli K12 NCTC8797 in water samples [PDF]

, 2009
The aim of this study was to investigate the impact on numbers of using different media for the enumeration of Escherichia coli subjected to stress, and to evaluate the use of different resuscitation methods on bacterial numbers. E. coli was subjected to
Bissonnette, Bissonnette, Bjergbaek, Byrd, Colwell, Darcan, Davies, Dukan, E. Kariptas, F. Saribiyik, Flint, Gameson, Herson, Hobbie, Jannasch, Jones, K. Isik, K.P. Flint, LeChevallier, Lim, McCleery, McFeters, Means, N. Sahin, Nilsson, Pagel, R. Özkanca, Ray, Reissbrodt, Roszak, Roszak, Tabor, Tillett, White, Xu, Özkanca, Özkanca, Özkanca   +37 more
core   +1 more source

Sequence determinants of RNA G‐quadruplex unfolding by Arg‐rich regions

FEBS Letters, EarlyView.
We show that Arg‐rich peptides selectively unfold RNA G‐quadruplexes, but not RNA stem‐loops or DNA/RNA duplexes. This length‐dependent activity is inhibited by acidic residues and is conserved among SR and SR‐related proteins (SRSF1, SRSF3, SRSF9, U1‐70K, and U2AF1).
Naiduwadura Ivon Upekala De Silva, Puspa Kunwar, Md Ibnul Rifat Rahman, Joanna Koryo Kwao, Nathan Lehman, Zihan Zhang, Trenton Paul, Claire Cheng, Nicholas Truex, Hui‐Ting Lee, Jun Zhang   +10 more
wiley   +1 more source

Mitochondrial Rab GAPs govern autophagosome biogenesis during mitophagy

eLife, 2014
Damaged mitochondria can be selectively eliminated by mitophagy. Although two gene products mutated in Parkinson’s disease, PINK1, and Parkin have been found to play a central role in triggering mitophagy in mammals, how the pre-autophagosomal isolation ...
Koji Yamano, Adam I Fogel, Chunxin Wang, Alexander M van der Bliek, Richard J Youle   +4 more
doaj   +1 more source

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

FEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li, Pedro A. Sánchez‐Murcia, Bernd Nidetzky   +2 more
wiley   +1 more source

The role of homogenization cycles and Poloxamer 188 on the quality of mitochondria isolated for use in mitochondrial transplantation therapy

Scientific Reports
Mitochondrial transplantation (MTx) offers a promising therapeutic approach to mitigate mitochondrial dysfunction in conditions such as ischemia–reperfusion (IR) injury.
Ryosuke Takegawa, Kei Hayashida, Atsushi Murao, Yusuke Endo, Cyrus E. Kuschner, Jacob Kazmi, Eriko Nakamura, Ping Wang, Lance B. Becker   +8 more
doaj   +1 more source