Results 61 to 70 of about 1,162,423 (312)

Proof of concept of using a membrane-sensing peptide for sEVs affinity-based isolation

Frontiers in Bioengineering and Biotechnology, 2023
Introduction: One main limitation in biomarker studies using EVs is the lack of a suitable isolation method rendering high yield and purity samples in a quick and easily standardized procedure.
Beatriz Benayas, Beatriz Benayas, Joaquín Morales, Alessandro Gori, Alessandro Strada, Paola Gagni, Roberto Frigerio, Carolina Egea, Pilar Armisén, Marina Cretich, María Yáñez-Mó   +10 more
doaj   +1 more source

Cultural methods of detection for microorganisms: recent advances and successes [PDF]

, 1997
Most microbiological methods require culture to allow organisms to recover or to selectively increase, and target organisms are identified by growth on specific agar media.
Jian, X., Watkins , J.
core  

Retrograde trafficking of Argonaute 2 acts as a rate-limiting step for de novo miRNP formation on endoplasmic reticulum–attached polysomes in mammalian cells [PDF]

, 2020
microRNAs are short regulatory RNAs in metazoan cells. Regulation of miRNA activity and abundance is evident in human cells where availability of target messages can influence miRNA biogenesis by augmenting the Dicer1-dependent processing of precursors ...
Barman, Bahnisikha, Bhattacharyya, Suvendra N., Bose, Mainak, Chakrabarty, Yogaditya, Chatterjee, Susanta   +4 more
core  

Transferrin receptor 1‐mediated iron uptake supports thermogenic activation in human cervical‐derived adipocytes

FEBS Letters, EarlyView.
In this study, we found that human cervical‐derived adipocytes maintain intracellular iron level by regulating the expression of iron transport‐related proteins during adrenergic stimulation. Melanotransferrin is predicted to interact with transferrin receptor 1 based on in silico analysis.
Rahaf Alrifai, Mizuki Seo, Gyath Karadsheh, Fachrur Rizal Mahendra, Máté Á Demény, Ferenc Győry, János András Mótyán, László Fésüs, Endre Kristóf, Rini Arianti   +9 more
wiley   +1 more source

Guidelines for plasma membrane protein detection by surface biotinylation

Molecules and Cells
: Plasma membrane proteins are crucial for signal transduction, trafficking, and cell-cell interactions, all of which are vital for cell survival. These proteins, including G-protein coupled receptors, ion channels, transporters, and receptors, are key ...
Jae Won Roh, Hye Won Choi, Heon Yung Gee
doaj   +1 more source

Towards a membrane proteome in Drosophila: a method for the isolation of plasma membrane

BMC Genomics, 2010
Background The plasma membrane (PM) is a compartment of significant interest because cell surface proteins influence the way in which a cell interacts with its neighbours and its extracellular environment.
Thomas Graham H, Stanley Bruce A, Khanna Mansi R   +2 more
doaj   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

A method for isolating and cryopreserving intact mitochondria with improved integrity and functionality

Biophysics and Physicobiology
Mitochondria isolated from cells are essential tools in biological research. However, many mitochondria are often damaged during the isolation process.
Arima Okutani, Jannatul Naima, Asaka Ogihara, Taihei Motoichi, Ikuroh Ohsawa, Yoshihiro Ohta   +5 more
doaj   +1 more source

Aspects of conceptualization, development, and simulation of an extracellular vesicles' bulk isolation device for cosmetic and therapeutic applications

Biomedical Engineering Advances
This paper discusses the concept and model to develop a prototype extracellular vesicle bulk-isolation device (EBID). It proposes an engineering design and potential ways to mitigate limitations in the currently available extracellular vesicle (EV ...
Franck M Senda, Gilles C Roy
doaj   +1 more source

Characterization of the newly isolated lytic bacteriophages KTN6 and KT28 and their efficacy against Pseudomonas aeruginosa biofilm [PDF]

, 2015
We here describe two novel lytic phages, KT28 and KTN6, infecting Pseudomonas aeruginosa, isolated from a sewage sample from an irrigated field near Wroclaw, in Poland.
Arabski, Michal, Augustyniak, Daria, Briers, Yves, Danis-Wlodarczyk, Katarzyna, Drulis-Kawa, Zuzanna, Duda, Katarzyna Anna, Gula, Grzegorz, Jang, Ho Bin, Lavigne, Rob, Majkowska-Skrobek, Grazyna, Olszak, Tomasz, Vandenheuvel, Dieter, Wasik, Slawomir   +12 more
core   +4 more sources