Results 21 to 30 of about 1,162,423 (312)
Adult skeletal muscle stem cell migration is mediated by a blebbing/amoeboid mechanism [PDF]
, 2011 Adult skeletal muscle possesses a resident stem cell population called satellite cells which are responsible for tissue repair following damage. Satellite cell migration is crucial in promoting rapid tissue regeneration but is a poorly understood ...
Collins-Hooper, Henry +4 more
core +1 more source
Effects of freezing and protein cross-linker on isolating membrane raft–associated proteins
BioTechniques, 2010 Since the discovery of cellular membrane rafts, the defining of these domains has remained ambiguous due to a great number of isolation procedures proposed for the extraction of the rafts from cells.
Kimberly Suzanne George +2 more
doaj +1 more source
Isolation and Characterization of Membrane Vesicles from Lactobacillus Species
Bio-Protocol, 2021 Throughout their life cycle, bacteria shed portions of their outermost membrane comprised of proteins, lipids, and a diversity of other biomolecules. These biological nanoparticles have been shown to have a range of highly diverse biological activities ...
Julie Caruana, Scott Dean, Scott Walper
doaj +1 more source
Domain-specific lipid distribution in macrophage plasma membranes
Journal of Lipid Research, 2005 Lipid rafts, defined as cholesterol- and sphingolipid-rich domains, provide specialized lipid environments understood to regulate the organization and function of many plasma membrane proteins.
Katharina Gaus +6 more
doaj +1 more source
Tissue engineering a fetal membrane [PDF]
, 2011 The aim of this study was to construct an artificial fetal membrane (FM) by combination of human amniotic epithelial stem cells (hAESCs) and a mechanically enhanced collagen scaffold containing encapsulated human amniotic stromal fibroblasts (hASFs ...
Adam M. Squires +11 more
core +1 more source
Distribution of chromaffin secretory vesicles, acetylcholinesterase, and lysosomal enzymes in sucrose and percoll gradients [PDF]
, 1984 Crude chromaffin secretory vesicles, obtained by differential centrifugation, were further purified on isotonic (Percoll) gradients. The chromaffin vesicle fractions recovered from the gradients contain acetylcholinesterase as well as lysosomal enzymes ...
Bowers +31 more
core +1 more source
A simplified method for the preparation of detergent-free lipid rafts
Journal of Lipid Research, 2005 Lipid rafts are small plasma membrane domains that contain high levels of cholesterol and sphingolipids. Traditional methods for the biochemical isolation of lipid rafts involve the extraction of cells with nonionic detergents followed by the separation ...
Jennifer L. Macdonald, Linda J. Pike
doaj +1 more source
Physiological Reports, 2020 Intact cardiomyocytes are used to investigate cardiac contractility and evaluate the efficacy of new therapeutic compounds. Primary enzymatic isolation of adult rodent cardiomyocytes has limitations, including low cardiomyocyte survival, which is likely ...
Teresa L. Czeiszperger +2 more
doaj +1 more source
Isolation and immunofluorescence staining of Aspergillus fumigatus conidia-containing phagolysosomes
STAR Protocols, 2021 Summary: The analysis of phagolysosomes within professional phagocytic cells is facilitated by their isolation. Here, we optimized a protocol for the isolation of intact phagolysosomes from macrophages infected with the spores of Aspergillus fumigatus ...
Marie Goldmann +4 more
doaj +1 more source
Isolation of Mycoplasma Membranes by Digitonin [PDF]
Journal of Bacteriology, 1972 The cell membrane of Mycoplasma hominis was isolated by lysing the cells with digitonin. Electron microscopy and chemical, density gradient, and electrophoretic analyses of the membrane proteins showed the membranes so obtained, like those isolated by osmotic lysis, to be relatively free of cytoplasmic contaminants.
S, Rottem, S, Razin
openaire +2 more sources