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Isothermal Amplification of Nucleic Acids
Chemical Reviews, 2015Isothermal amplification of nucleic acids is a simple process that rapidly and efficiently accumulates nucleic acid sequences at constant temperature. Since the early 1990s, various isothermal amplification techniques have been developed as alternatives to polymerase chain reaction (PCR).
Yongxi, Zhao +4 more
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Ramification amplification: A novel isothermal DNA amplification method
Molecular Diagnosis, 2001We have developed a novel isothermal DNA amplification method with an amplification mechanism quite different from conventional PCR. This method uses a specially designed circular probe (C-probe) in which the 3' and 5' ends are brought together in juxtaposition by hybridization to a target.
D Y, Zhang +3 more
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Multiplexed isothermal nucleic acid amplification
Analytical Biochemistry, 2018Multiplexed isothermal amplification and detection of nucleic acid sequences and biomarkers is of increasing importance in diverse areas including advanced diagnostics, food quality control and environmental monitoring. Whilst there are several very elegant isothermal amplification approaches, multiplexed amplification remains a challenge, requiring ...
Olena, Mayboroda +2 more
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Isothermal DNA amplification in vitro: the helicase-dependent amplification system
Cellular and Molecular Life Sciences, 2009Since the development of polymerase chain reaction, amplification of nucleic acids has emerged as an elemental tool for molecular biology, genomics, and biotechnology. Amplification methods often use temperature cycling to exponentially amplify nucleic acids; however, isothermal amplification methods have also been developed, which do not require ...
Yong-Joo, Jeong +2 more
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