Results 51 to 60 of about 62,486 (251)

Delayed poration after ultrashort electric pulses on Jurkat cell

, 2007
Electrical properties of cancer cells have been studied widely to understand cancer. The increase in electrical conductivity of Jurkat cell suspension after ultrashort electric pulses has been considered as an important phenomenon of the interaction ...
Daly, K., Chen, G.
core   +1 more source

Jurkat T-cell lines exhibit marked genomic instability affecting karyotype, mutational profile, gene expression, immunophenotype and function

Scientific Reports
The Jurkat cell line, derived from a case of T-cell lymphoblastic leukaemia, is widely employed as a model T-cell for biomedical research, including for the preclinical characterisation of cellular therapies such as chimeric antigen receptor T-cells ...
Andrew Wilson, Nathaniel Dasyam, Felix O’Hagan, Dianna Farrell, Claire Turner, Anne Jay, Alfonso Schmidt, Rachel Beddow, Tina Lillis, Tom Chin, Rachel Perret, Michelle McCulley, Robert Weinkove   +12 more
doaj   +1 more source

Zerumbone Induces G2/M Cell Cycle Arrest and Apoptosis via Mitochondrial Pathway in Jurkat cell Line

Natural Product Communications, 2014
This investigation determined the anticancer properties of zerumbone (ZER) on the human T-cell (Jurkat) line using the MTT assay, microscopic evaluations, flow cytometric analyses, and caspase activity estimations.
Heshu Sulaiman Rahman, Abdullah Rasedee, Max Stanley Chartrand, Hemn Hassan Othman, Swee Keong Yeap, Farideh Namvar   +5 more
doaj   +1 more source

Polyunsaturated Fatty Acid Biosynthesis Involving Δ8 Desaturation and Differential DNA Methylation of FADS2 Regulates Proliferation of Human Peripheral Blood Mononuclear Cells

Frontiers in Immunology, 2018
Polyunsaturated fatty acids (PUFAs) are important for immune function. Limited evidence indicates that immune cell activation involves endogenous PUFA synthesis, but this has not been characterised.
Charlene M. Sibbons, Charlene M. Sibbons, Nicola A. Irvine, J. Eduardo Pérez-Mojica, Philip C. Calder, Karen A. Lillycrop, Barbara A. Fielding, Graham C. Burdge   +7 more
doaj   +1 more source

Dose-dependent thresholds of 10-ns electric pulse induced plasma membrane disruption and cytotoxicity in multiple cell lines. [PDF]

PLoS ONE, 2011
In this study, we determined the LD(50) (50% lethal dose) for cell death, and the ED(50) (50% of cell population staining positive) for propidium (Pr) iodide uptake, and phosphatidylserine (PS) externalization for several commonly studied cell lines ...
Bennett L Ibey, Caleb C Roth, Andrei G Pakhomov, Joshua A Bernhard, Gerald J Wilmink, Olga N Pakhomova   +5 more
doaj   +1 more source

Using the Jurkat reporter T cell line for evaluating the functionality of novel chimeric antigen receptors

Frontiers in Molecular Medicine, 2023
Background: T cells that are genetically modified with chimeric antigen receptor (CAR) hold promise for immunotherapy of cancer. Currently, there are intense efforts to improve the safety and efficacy of CAR T cell therapies against liquid and solid ...
Farhana Jahan, Jan Koski, Diana Schenkwein, Seppo Ylä-Herttuala, Seppo Ylä-Herttuala, Helka Göös, Sini Huuskonen, Markku Varjosalo, Pilvi Maliniemi, Judith Leitner, Peter Steinberger, Hans-Jörg Bühring, Kim Vettenranta, Kim Vettenranta, Matti Korhonen   +14 more
doaj   +1 more source

Migration of Jurkat cells in response to CypA

The FASEB Journal, 2012
Cyclophilins (CyPs) are ubiquitously distributed proteins, identified initially as the main binding target for cyclosporine A (CsA). A potential contribution of cyclophilin in inflammatory diseases is suggested from several studies. The activities of secreted cyclophilins are related to interactions with CD147, a transmembrane glycoprotein that plays a
Rosalyn A Jurjus, Tatiana Pushkarsky, Michael Bukrinsky   +2 more
openaire   +1 more source

Effects of canola and corn oil mimetic on Jurkat cells [PDF]

Lipids in Health and Disease, 2011
The Western diet is high in omega-6 fatty acids and low in omega-3 fatty acids. Canola oil contains a healthier omega 3 to omega 6 ratio than corn oil. Jurkat T leukemia cells were treated with free fatty acids mixtures in ratios mimicking that found in commercially available canola oil (7% α-linolenic, 30% linoleic, 54% oleic) or corn oil (59 ...
Akinsete Juliana A, Fazio Kayla, Ion Gabriela, Hardman W Elaine   +3 more
openaire   +3 more sources

The pmutEGFP-TAG182 SHM vector was tested in Jurkat and Jurkat-AID cell lines.

, 2013
Twenty hours after transfection cells were analyzed on a FACS Scan. Cells that have reverted the stop codon of one or more copies of the vector appear fluorescent.
Tihana Jovanic (84063), Géraldine Attal-Bonnefoy (84065), François Rougeon (84067), Olivier Leclercq (84066), Benjamin Roche (84064)   +4 more
core   +1 more source

A Synthetic Platform for Antibody Junctional Diversification Beyond Natural Constraints

Advanced Science, EarlyView.
ARESEC is a synthetic platform that reconstructs functional V(D)J recombination in HEK293T cells. By introducing RSS elements and co‐expressing RAG1/2 and TdT across all three antibody CDRs, it enables programmable junctional diversification and direct generation of high‐affinity variants targeting PD‐1/PD‐L1, overcoming the evolutionary constraints of
Wang Liu, Jun Yang, Yuhang Zhang, Xiaoli Xue   +3 more
wiley   +1 more source