Results 81 to 90 of about 8,462,139 (217)

The Structural Basis of Force Generation by the Mitotic Motor Kinesin-5 [PDF]

Biophysical Journal, 2012
Kinesin-5 is required for forming the bipolar spindle during mitosis. Its motor domain, which contains nucleotide and microtubule binding sites and mechanical elements to generate force, has evolved distinct properties for its spindle-based functions.
Goulet, Adeline, Behnke-Parks, William, Sindelar, Charles, Major, Jennifer, Rosenfeld, Steven, Moores, Carolyn   +5 more
openaire   +5 more sources

In vivo Imaging of oskar mRNA transport reveals the mechanism of posterior localization [PDF]

, 2008
oskar mRNA localization to the posterior of the Drosophila oocyte defines where the abdomen and germ cells form in the embryo. Although this localization requires microtubules and the plus end-directed motor, kinesin, its mechanism is controversial and ...
Zimyanin, Vitaly L, Gilchrist, Michael J., Pecreaux, J, Davis, Ilan, Zimyanin, Vitaly L., Gilchrist, Michael J, Clark, Alejandra, St Johnston, D, Belaya, K, Pecreaux, Jacques, Clark, A, Gilchrist, MJ, Davis, I, St Johnston, Daniel, Zimyanin, VL, Belaya, Katsiaryna, Johnston, Daniel St   +16 more
core   +1 more source

Curcumin improves synaptic plasticity impairment induced by HIV-1gp120 V3 loop

Neural Regeneration Research, 2015
Curcumin has been shown to significantly improve spatial memory impairment induced by HIV-1 gp120 V3 in rats, but the electrophysiological mechanism remains unknown.
Ling-ling Shen, Ming-liang Jiang, Si-si Liu, Min-chun Cai, Zhong-qiu Hong, Li-qing Lin, Yan-yan Xing, Gui-lin Chen, Rui Pan, Li-juan Yang, Ying Xu, Jun Dong   +11 more
doaj   +1 more source

Kinesin-5: Cross-bridging mechanism to targeted clinical therapy [PDF]

Gene, 2013
Kinesin motor proteins comprise an ATPase superfamily that works hand in hand with microtubules in every eukaryote. The mitotic kinesins, by virtue of their potential therapeutic role in cancerous cells, have been a major focus of research for the past 28 years since the discovery of the canonical Kinesin-1 heavy chain.
Edward J, Wojcik, Rebecca S, Buckley, Jessica, Richard, Liqiong, Liu, Thomas M, Huckaba, Sunyoung, Kim   +5 more
openaire   +2 more sources

A surprising twist

eLife, 2014
X-ray crystallography has revealed an unusual structural element in kinesin-5 motor proteins.
Nikta Fakhri, Christoph F Schmidt
doaj   +1 more source

IMACulate(DE3), an E. coli Strain for High Purity His‐Tagged Protein Purifications

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Immobilised Metal Affinity Chromatography (IMAC) is widely used to purify his‐tagged recombinant proteins from Escherichia coli. However, endogenous contaminants with histidine clusters, such as GFAT and PDH E1 proteins, are often co‐purified with the target protein.
Jia Q. Truong, Dingyi Yu, Nan Hao, Chris Langendorf, Keith. E. Shearwin, Jessica K. Holien   +5 more
wiley   +1 more source

Chromosome 20q Amplification Regulates in Vitro Response to Kinesin-5 Inhibitor

Cancer Informatics, 2008
We identified gene expression signatures predicting responsiveness to a Kinesin-5 (KIF11) inhibitor (Kinesin-5i) in cultured colon tumor cell lines.
Peter S. Linsley, Steven R. Bartz, Hongyue Dai, Matthew Biery, Teresa Ward, Sumire Kobayashi, Mao Mao, Aimee L. Jackson   +7 more
doaj  

Stem Cell Ophthalmology Treatment Study (SCOTS) for retinal and optic nerve diseases: a preliminary report

Neural Regeneration Research, 2015
In this report, we present the results of a single patient with optic neuropathy treated within the Stem Cell Ophthalmology Treatment Study (SCOTS). SCOTS is an Institutional Review Board approved clinical trial and is the largest ophthalmology stem cell
Jeffrey N Weiss, Steven Levy, Alexis Malkin   +2 more
doaj   +1 more source

Purification, Fluorescent Labeling, and Detyrosination of Mammalian Cell Tubulin for Biochemical Assays

Cytoskeleton, EarlyView.
ABSTRACT Microtubules play essential roles in numerous cellular processes. All microtubules are built from the protein tubulin, yet individual microtubules can differ spatially and temporally due to their tubulin isotype composition and post‐translational modifications (PTMs).
Ezekiel C. Thomas, Yang Yue, Morgan L. Pimm, Takashi Hotta, Ryoma Ohi, Kristen J. Verhey   +5 more
wiley   +1 more source

Recombinant Monoclonal Antibodies for Detecting the Tubulin Post‐Translational Modifications Glutamylation and Lysine‐40 Acetylation

Cytoskeleton, EarlyView.
ABSTRACT Post‐translational modifications (PTMs) to tubulin subunits in microtubule filaments are thought to comprise a component of the tubulin code that specifies microtubule functions in cell physiology and animal development. Acetylation of Lysine‐40 (K40) on α‐tubulin (αTub‐K40ac) and glutamylation of both α‐ and β‐tubulin are two tubulin PTMs of ...
Lynne Blasius, Ezekiel C. Thomas, Erik H. Collet, Erin Jenson, Chad G. Pearson, Ryoma Ohi, Kristen J. Verhey   +6 more
wiley   +1 more source