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Hydroxyurea-Induced Synchrony of DNA Replication in the Kinetoplastida
Experimental Cell Research, 1994We have developed a reliable and reproducible method to induce synchrony of the DNA synthetic cycle in the Kinetoplastida. The method involves treatment of cultures with 20 mM hydroxyurea (HU) and fetal bovine serum. Both stationary-phase and exponential-phase cultures can be synchronized.
N, Galanti +3 more
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Freeze‐fracture study of Bodo sp. (Kinetoplastida: Bodonidae)
Microscopy Research and Technique, 1995AbstractFreeze‐fracture technique was used to analyse the structure of conventionally fixed and quickly frozen Bodo sp., a free‐living kinetoplastid. In the former method, chemically fixed and cryopreserved cells presented a corrugated membrane pattern in the flagella and cell body surfaces.
R C, Vommaro, M, Attias, W, de Souza
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Evidence for the functioning of cytochrome o in kinetoplastida
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1974Abstract Action spectra for the photochemical relief of CO-inhibition of respiration have provided evidence for two functional terminal oxidases, cytochrome a 3 and cytochrome o in several Kinetoplastida genera. These include: Trypanosoma mega, Blastocrithidia culicis and Leishmania tarentolae .
P, Kronick, G C, Hill
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Mechanisms of stage-regulated gene expression in kinetoplastida
Parasitology Today, 1995During their life cycle, trypanosomatid parasites of mammals encounter substantially different environments in their hosts and insect vectors, to which they must adapt by undergoing a series of differentiation processes. At the molecular level, these processes must be the direct result of an elaborate series of changes in stage-regulated expression of ...
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Cyanide-resistant respiration and a branched cytochrome system in Kinetoplastidae
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1973Abstract Two functional terminal oxidases, cytochrome aa 3 and cytochrome o , are present in the cytochrome system in cyanide-sensitive trypanosomatids. The organisms examined included Crithidia fasciculata, Leptomonas sp., Blastocrithidia culicis, Herpetomonas muscarum, Leishmania tarentolae, Trypanosoma lewisi, Trypanosoma conorhini and ...
G C, Hill, G A, Cross
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The morphology of ovine Trypanosoma melophagium (zoomastigophorea: kinetoplastida).
The Journal of protozoology, 1984Morphologic and biometric data on bloodstream stages of Trypanosoma melophagium are presented. An increasing parasitemia with 111 trypomastigote stages of T. melophagium were found in Giemsa-stained thin blood smears taken from a splenectomized, cortisone-treated sheep recently infested with Melophagus ovinus infected with T. melophagium .
G, Büscher, K T, Friedhoff
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Current concepts in stage-regulated gene expression in kinetoplastida
Parasitology Today, 1990The different physiological environments provided by the hosts probably generated a strong selective force for the evolution of differential expression of gene products in digenetic kinetoplastid parasites. Indeed, the sorts of molecules that would be predicted to be stage-regulated have been found to be differentially expressed.
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[Cell fusion in Leishmania (Kinetoplastida, Trypanosomatidae)].
Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie, 1990Cell fusion in Leishmania infantum and L. tropica was recorded by means of a camera and a video system. Promastigotes were obtained from cultures in both cases. Fusion began with attachment of the posterior extremities of two ovoid flagellates. Complete fusion, with disappearance of adjacent cell membranes took about five minutes.
G, Lanotte, J A, Rioux
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2021
Aims. The purpose of this study was a reconstruction and verification of spatial structures of tubulin molecules from representatives of Diplomonadida, Kinetoplastida, Amoebida, Saccharomycetes and Microsporea to determine differences in their spatial structures and clarify the mechanism of their interaction with antimitotic agents. Methods.
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Aims. The purpose of this study was a reconstruction and verification of spatial structures of tubulin molecules from representatives of Diplomonadida, Kinetoplastida, Amoebida, Saccharomycetes and Microsporea to determine differences in their spatial structures and clarify the mechanism of their interaction with antimitotic agents. Methods.
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Kinetoplastida: model organisms for simple autophagic pathways?
Methods in enzymology, 2009Phylogenetic analyses based on defined proteins or different RNA species have revealed that the order kinetoplastida belongs to the early-branching eukaryotes and may thus contain organisms in which complex cellular events are easier to analyze. This view was further supported by results from a bioinformatic survey that suggested that nearly half of ...
Viola, Denninger +7 more
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