Results 1 to 10 of about 2,562 (158)

Crystal structures of the HCl salts of pseudopeptidic macrocycles display “knobs into holes” hydrophobic interactions between aliphatic side chains [PDF]

CrystEngComm, 2009
The crystal structures of three HCl salts of pseudopeptidic macrocycles show a H-bonding tubular stack of the rings, where the amino acidic side chains (Val, Leu, Ile) display an inter-columnar “knobs into holes” hydrophobic interaction ...
Ignacio Alfonso, Michael Bolte, M Isabel Burguete   +2 more
exaly   +6 more sources

Production of bispecific antibodies in "knobs-into-holes" using a cell-free expression system. [PDF]

MAbs, 2015
Bispecific antibodies have emerged in recent years as a promising field of research for therapies in oncology, inflammable diseases, and infectious diseases. Their capability of dual target recognition allows for novel therapeutic hypothesis to be tested, where traditional mono-specific antibodies would lack the needed mode of target engagement.
Xu Y, Lee J, Tran C, Heibeck TH, Wang WD, Yang J, Stafford RL, Steiner AR, Sato AK, Hallam TJ, Yin G.   +10 more
europepmc   +5 more sources

Heavy and light chain pairing of bivalent quadroma and knobs-into-holes antibodies analyzed by UHR-ESI-QTOF mass spectrometry. [PDF]

MAbs, 2016
The quadroma antibody represents the first attempt to produce a bispecific heterodimeric IgG antibody by somatic fusion of 2 hybridoma cells each expressing monoclonal antibodies with distinctive specificities. However, because of random heavy and light chain pairing, the desired functional bispecific antibody represents only a small fraction of the ...
Schaefer W, Völger HR, Lorenz S, Imhof-Jung S, Regula JT, Klein C, Mølhøj M.   +6 more
europepmc   +5 more sources

Knobs-into-holes antibody production in mammalian cell lines reveals that asymmetric afucosylation is sufficient for full antibody-dependent cellular cytotoxicity. [PDF]

MAbs, 2013
Knobs-into-holes is a well-validated heterodimerization technology for the third constant domain of an antibody. This technology has been used to produce a monovalent IgG for clinical development (onartuzumab) and multiple bispecific antibodies. The most advanced uses of this approach, however, have been limited to E.
Shatz W, Chung S, Li B, Marshall B, Tejada M, Phung W, Sandoval W, Kelley RF, Scheer JM.   +8 more
europepmc   +5 more sources

Effect of allotypic variation of human IgG1 on the thermal stability of disulfide-linked knobs-into-holes mutants of the Fc for stable bispecific antibody design. [PDF]

Antib Ther, 2019
AbstractBackgroundDisulfide-linked knobs-into-holes (dKiH) mutation is a well-validated antibody engineering technique to force heterodimer formation of different Fcs for efficient production of bispecific antibodies. An artificial disulfide bond is created between mutated cysteine residues in CH3 domain of human IgG1 Fc whose positions are 354 of the “
Akiba H, Satoh R, Nagata S, Tsumoto K.
europepmc   +5 more sources

Crystal structure of an anti-Ang2 CrossFab demonstrates complete structural and functional integrity of the variable domain. [PDF]

PLoS ONE, 2013
Bispecific antibodies are considered as a promising class of future biotherapeutic molecules. They comprise binding specificities for two different antigens, which may provide additive or synergistic modes of action.
Sebastian Fenn, Christian B Schiller, Julia J Griese, Harald Duerr, Sabine Imhof-Jung, Christian Gassner, Joerg Moelleken, Joerg Thomas Regula, Wolfgang Schaefer, Markus Thomas, Christian Klein, Karl-Peter Hopfner, Hubert Kettenberger   +12 more
doaj   +6 more sources

Nonclinical immunogenicity risk assessment for knobs-into-holes bispecific IgG1 antibodies

mAbs
Bispecific antibodies, including bispecific IgG, are emerging as an important new class of antibody therapeutics. As a result, we, as well as others, have developed engineering strategies designed to facilitate the efficient production of bispecific IgG ...
Wen-Ting K. Tsai, Yinyin Li, Zhaojun Yin, Peter Tran, Qui Phung, Zhenru Zhou, Kun Peng, Dan Qin, Sien Tam, Christoph Spiess, Jochen Brumm, Manda Wong, Zhengmao Ye, Patrick Wu, Sivan Cohen, Paul J. Carter   +15 more
doaj   +3 more sources

Construction of bispecific antibodies by specific pairing between the heavy chain and the light chain using removable SpyCatcher/SnoopCatcher units [PDF]

Journal of Biological Engineering
During the production of bispecific antibodies (bsAbs), nonspecific pairing results in low yields of target bsAb molecules, an issue known as the “mispairing problem.” Several antibody engineering techniques have been developed to overcome mispairing ...
Jyunna Yoshida, Yuki Kato, Ai Isogawa, Yoshikazu Tanaka, Izumi Kumagai, Ryutaro Asano, Takeshi Nakanishi, Koki Makabe   +7 more
doaj   +2 more sources

Physicochemical and biological characterization of a bispecific antibody in a CrossMab/KIH format that targets EGFR and VEGF-A [PDF]

Frontiers in Immunology
IntroductionBispecific antibodies (BsAbs) are a class of antibody therapeutics engineered in various molecular formats to bind two distinct antigens and potentially mediate multiple biological effects.
Safiat Ayinde, Shraboni Dutta, Nishant Mohan, Wen Jin Wu   +3 more
doaj   +2 more sources

Rapid affinity-based purification of multi-specific antibodies using Kappa Select and Protein L [PDF]

mAbs
Multispecific antibodies (msAbs) are becoming more prevalent as formats of choice for therapeutic antibody development due to their ability to modulate multiple biological targets.
Kalie Mix, Tingwan Sun, Brian Hall, Jocelyn Newton, Christina Eng, Yongjing Guo, David Reczek   +6 more
doaj   +2 more sources