Construction of bispecific antibodies by specific pairing between the heavy chain and the light chain using removable SpyCatcher/SnoopCatcher units [PDF]
Journal of Biological EngineeringDuring the production of bispecific antibodies (bsAbs), nonspecific pairing results in low yields of target bsAb molecules, an issue known as the “mispairing problem.” Several antibody engineering techniques have been developed to overcome mispairing ...
Jyunna Yoshida +7 more
doaj +2 more sources
Physicochemical and biological characterization of a bispecific antibody in a CrossMab/KIH format that targets EGFR and VEGF-A [PDF]
Frontiers in ImmunologyIntroductionBispecific antibodies (BsAbs) are a class of antibody therapeutics engineered in various molecular formats to bind two distinct antigens and potentially mediate multiple biological effects.
Safiat Ayinde +3 more
doaj +2 more sources
Nonclinical immunogenicity risk assessment for knobs-into-holes bispecific IgG 1 antibodies
MAbsBispecific antibodies, including bispecific IgG, are emerging as an important new class of antibody therapeutics. As a result, we, as well as others, have developed engineering strategies designed to facilitate the efficient production of bispecific IgG ...
Wen-Ting K Tsai, Yinyin Li, Qui T Phung
exaly +3 more sources
Rapid affinity-based purification of multi-specific antibodies using Kappa Select and Protein L [PDF]
mAbsMultispecific antibodies (msAbs) are becoming more prevalent as formats of choice for therapeutic antibody development due to their ability to modulate multiple biological targets.
Kalie Mix +6 more
doaj +2 more sources
Heavy and light chain pairing of bivalent quadroma and knobs-into-holes antibodies analyzed by UHR-ESI-QTOF mass spectrometry [PDF]
MAbs, 2016 Wolfgang Schaefer +2 more
exaly +2 more sources
Knobs-into-holes antibody production in mammalian cell lines reveals that asymmetric afucosylation is sufficient for full antibody-dependent cellular cytotoxicity [PDF]
MAbs, 2013 Whitney Shatz +2 more
exaly +2 more sources