Results 11 to 20 of about 2,068 (144)
STAR Protocols, 2021 Summary: Follistatin-like 3 (FSTL3) is an endogenous antagonist against transforming growth factor-β family ligands. Monovalent FSTL3-Fc fusion protein (mono-FSTL3-Fc) generated with knobs-into-holes technology overcomes limitations of current anti ...
Takayuki Ozawa +2 more
doaj +1 more source
Effective flow-through polishing strategies for knob-into-hole bispecific antibodies
Bioresources and Bioprocessing, 2022 AbstractBispecific antibodies (bsAbs), though possessing great therapeutic potential, are extremely challenging to obtain at high purity within a limited number of scalable downstream processing steps. Complementary to Protein A chromatography, polishing strategies play a critical role at removing the remaining high molecular weight (HMW) and low ...
Serene W. Chen +4 more
openaire +3 more sources
Building coiled coils with polar cores. [PDF]
Protein SciAbstract Coiled coils are formed by α‐helices winding around each other into superhelical bundles. They are characterized by a specific geometry of interaction, called knobs‐into‐holes, in which residues in the core of the structure mesh regularly along a seam that runs the length of the helices.
Hartmann MD +3 more
europepmc +2 more sources
Crystal structure of an anti-Ang2 CrossFab demonstrates complete structural and functional integrity of the variable domain. [PDF]
PLoS ONE, 2013 Bispecific antibodies are considered as a promising class of future biotherapeutic molecules. They comprise binding specificities for two different antigens, which may provide additive or synergistic modes of action.
Sebastian Fenn +12 more
doaj +1 more source
An integrated approach for characterizing immunogenic responses toward a bispecific antibody
mAbs, 2021 Bispecific antibodies (bsAbs) recognize and bind two different targets or two epitopes of the same antigen, making them an attractive diagnostic and treatment modality.
Sivan Cohen +14 more
doaj +1 more source
iScience, 2021 Summary: Targeting the signaling pathway of growth differentiation factor 8 (GDF8), also known as myostatin, has been regarded as a promising strategy to increase muscle mass in the elderly and in patients.
Takayuki Ozawa +7 more
doaj +1 more source
iScience, 2022 Summary: Ebola virus (EBOV) and Marburg virus (MARV) are highly pathogenic viruses in humans, against which approved antivirals are lacking. During EBOV and MARV infection, coiled-coil mediated oligomerization is essential for the virion protein 35 (VP35)
Luca Zinzula +6 more
doaj +1 more source
IMGT® Nomenclature of Engineered IGHG Variants Involved in Antibody Effector Properties and Formats
Antibodies, 2022 The constant region of the immunoglobulin (IG) or antibody heavy gamma chain is frequently engineered to modify the effector properties of the therapeutic monoclonal antibodies.
Marie-Paule Lefranc, Gérard Lefranc
doaj +1 more source
Journal of Experimental & Clinical Cancer Research, 2019 Background Human epidermal growth factor receptor 2 (HER2) is overexpressed in multiple cancers, which is associated with poor prognosis. Herceptin and other agents targeting HER2 have potent antitumor efficacy in patients with HER2-positive cancers ...
Shengnan Yu +9 more
doaj +1 more source
Highly Efficient Mono-Functionalization of Knob-in-Hole Antibodies with Strain-Promoted Click Chemistry [PDF]
ACS Omega, 2019 Knob-in-hole antibodies can be utilized to introduce a single tag for chemo-enzymatic functionalization. By either introducing a single C-terminal sortase tag (sortase-tag expressed protein ligation) or tyrosine tag (G4Y), mono-functionalization of the monoclonal antibody trastuzumab was achieved rapidly and in high yields.
Bruins, Jorick J. +5 more
openaire +5 more sources