Functional Coiled-Coil-like Assembly by Knob-into-Hole Packing of Single Heptad Repeat [PDF]
ACS Nano, 2019 Coiled-coil peptides represent the principal building blocks for structure-based design of bionanomaterials. The sequence-structure relationship and precise nanoscale ordering of the coiled-coil helices originate from the knob-into-hole (KIH) packing of side chains.
Sudipta Mondal +5 more
+12 more sources
Engineered protein G variants for multifunctional antibody-based assemblies. [PDF]
Protein SciWe have developed a portfolio of antibody-based modules that can be prefabricated as standalone units and snapped together in plug-and-play fashion to create uniquely powerful multifunctional assemblies.
Slezak T +5 more
europepmc +3 more sources
Effective flow-through polishing strategies for knob-into-hole bispecific antibodies
Bioresources and Bioprocessing, 2022 AbstractBispecific antibodies (bsAbs), though possessing great therapeutic potential, are extremely challenging to obtain at high purity within a limited number of scalable downstream processing steps. Complementary to Protein A chromatography, polishing strategies play a critical role at removing the remaining high molecular weight (HMW) and low ...
Serene W. Chen +4 more
openaire +3 more sources
Bispecific antibodies with Fab-arms featuring exchanged antigen-binding constant domains
Biochemistry and Biophysics Reports, 2021 Monoclonal antibodies can acquire the property of engagement of a second antigen via fusion methods or modification of their CDR loops, but also by modification of their constant domains, such as in the mAb2 format where a set of mutated amino acid ...
Filippo Benedetti +5 more
doaj +1 more source
STAR Protocols, 2021 Summary: Follistatin-like 3 (FSTL3) is an endogenous antagonist against transforming growth factor-β family ligands. Monovalent FSTL3-Fc fusion protein (mono-FSTL3-Fc) generated with knobs-into-holes technology overcomes limitations of current anti ...
Takayuki Ozawa +2 more
doaj +1 more source
Detailed Analytical Characterization of a Bispecific IgG1 CrossMab Antibody of the Knob-into-Hole Format Applying Various Stress Conditions Revealed Pronounced Stability [PDF]
ACS Omega, 2022 In recent years, a variety of new antibody formats have been developed. One of these formats allows the binding of one type of antibody to two different epitopes. This can for example be achieved by introduction of the "knob-into-hole" format and a combined CrossMab approach.
Ingrid Grunert +8 more
openaire +4 more sources
Rapsyn's knobs and holes: eight tetratrico peptide repeats. [PDF]
Biochem J, 1996 Ponting CC, Phillips C.
europepmc +2 more sources
An integrated approach for characterizing immunogenic responses toward a bispecific antibody
mAbs, 2021 Bispecific antibodies (bsAbs) recognize and bind two different targets or two epitopes of the same antigen, making them an attractive diagnostic and treatment modality.
Sivan Cohen +14 more
doaj +1 more source
‘Knobs-into-holes’ engineering of antibody CH3 domains for heavy chain heterodimerization [PDF]
"Protein Engineering, Design and Selection", 1996 'Knobs-into-holes' was originally proposed by Crick in 1952 as a model for the packing of amino acid side chains between adjacent alpha-helices. 'Knobs-into-holes' is demonstrated here as a novel and effective design strategy for engineering antibody heavy chain homodimers for heterodimerization.
J B, Ridgway, L G, Presta, P, Carter
openaire +2 more sources
iScience, 2021 Summary: Targeting the signaling pathway of growth differentiation factor 8 (GDF8), also known as myostatin, has been regarded as a promising strategy to increase muscle mass in the elderly and in patients.
Takayuki Ozawa +7 more
doaj +1 more source