Results 41 to 50 of about 2,562 (158)

Inhibition of peptide aggregation by means of enzymatic phosphorylation [PDF]

, 2016
As is the case in numerous natural processes, enzymatic phosphorylation can be used in the laboratory to influence the conformational populations of proteins.
Broncel, Malgorzata, Folmert, Kristin, Koksch, Beate, Siegert, Mary-Ann, Ullrich, Christopher H., von Berlepsch, Hans   +5 more
core   +2 more sources

Fc Engineering for Developing Therapeutic Bispecific Antibodies and Novel Scaffolds [PDF]

, 2017
Therapeutic monoclonal antibodies have become molecules of choice to treat autoimmune disorders, inflammatory diseases, and cancer. Moreover, bispecific/multispecific antibodies that target more than one antigen or epitope on a target cell or recruit ...
Abhishek Saxena, Donghui Wu, Hongyan Liu, Sachdev S. Sidhu   +3 more
core   +1 more source

Origin of a folded repeat protein from an intrinsically disordered ancestor. [PDF]

, 2016
Repetitive proteins are thought to have arisen through the amplification of subdomain-sized peptides. Many of these originated in a non-repetitive context as cofactors of RNA-based replication and catalysis, and required the RNA to assume their active ...
Abe, Adams, Alva, Alva, Alva, Anantharaman, Apic, Aurora, Bansal, Bansal, Berman, Bernhardt, Biegert, Binz, Blundell, Boratyn, Broom, Bubunenko, Burton, Camacho, Chaudhuri, Chen, Coquille, Cortajarena, Cowtan, Crick, Crooks, D'Andrea, Das, Das, de Juan, Di Domenico, Dosztányi, Dosztányi, Doyle, Dunin-Horkawicz, Dyson, Eck, Eddy, Emsley, Finn, Forrer, Fox, Fox, Gilbert, Gorodkin, Grimsley, Habchi, Hsiao, Huson, Iwaya, Kabsch, Kabsch, Kajander, Kajava, Karpenahalli, Katibah, Keefe, Keiski, Kobe, Kohl, Koonin, Kopec, Krachler, Kumar, Kyrpides, Kyrpides, Lamb, Larkin, Laskowski, Lee, Linding, Lunelli, Lupas, Magliery, Main, Main, Main, McLachlan, McLachlan, Morcos, Mosavi, Murzin, Nguyen, Noble, Ohtani, Oldfield, Orengo, Park, Parmeggiani, Paterakis, Peng, Ponting, Ramarao, Ranea, Remmert, Romero, Rämisch, Sawyer, Schluenzen, Schrödinger,, Scott, Serasinghe, Shatsky, Sheldrick, Shen, Sikorski, Stumpp, Söding, Söding, Tobin, Vagin, Varadi, Wei, Wootton, Wootton, Zeytuni, Zeytuni, Zeytuni, Zhang   +119 more
core   +3 more sources

Socket2:A Program for Locating, Visualising, and Analysing Coiled-coil Interfaces in Protein Structures [PDF]

, 2021
MotivationProtein–protein interactions are central to all biological processes. One frequently observed mode of such interactions is the α-helical coiled coil (CC).
Kumar, Prasun, Woolfson, Derek N
core   +2 more sources

Muscle weakness in TPM3-myopathy is due to reduced Ca2+-sensitivity and impaired acto-myosin cross-bridge cycling in slow fibres. [PDF]

, 2015
Dominant mutations in TPM3, encoding α-tropomyosin(slow), cause a congenital myopathy characterized by generalized muscle weakness. Here, we used a multidisciplinary approach to investigate the mechanism of muscle dysfunction in 12 TPM3-myopathy patients.
Beggs, AH, Clarke, NF, Cooper, ST, de Winter, JM, Ilkovski, B, Klinge, L, Marston, SB, McNamara, E, Mokbel, N, North, KN, Nowak, KJ, Ottenheijm, CA, Ravenscroft, G, Rendu, J, Yuen, M   +14 more
core   +1 more source

Probabilistic grammatical model of protein language and its application to helix-helix contact site classification [PDF]

, 2013
BACKGROUND: Hidden Markov Models power many state‐of‐the‐art tools in the field of protein bioinformatics. While excelling in their tasks, these methods of protein analysis do not convey directly information on medium‐ and long‐range residue‐residue ...
Dyrka, Witold, Jean‐Christophe Nebel, Malgorzata Kotulska, Witold WitoldDyrka   +3 more
core   +5 more sources

Exploring the atomic structure and conformational flexibility of a 320 Å long engineered viral fiber using X-ray crystallography. [PDF]

, 2014
Protein fibers are widespread in nature, but only a limited number of high-resolution structures have been determined experimentally. Unlike globular proteins, fibers are usually recalcitrant to form three-dimensional crystals, preventing single-crystal ...
Bhardwaj, Anshul, Casjens, Sherwood R, Cingolani, Gino   +2 more
core   +2 more sources

A human IgE bispecific antibody shows potent cytotoxic capacity mediated by monocytes [PDF]

, 2022
The generation of bispecific antibodies (bsAbs) targeting two different antigens opens a new level of specificity and, compared to mAbs, improved clinical efficacy in cancer therapy.
Berraondo, Pedro, Blokhuis, Bart, Halabi, Samer, Redegeld, Frank A.M., Russo-Cabrera, Joan Salvador, Vukovic, Natasa, Zaiss, Dietmar M.W.   +6 more
core   +3 more sources

Non-affinity platform for processing knob-into-hole bispecific antibody

Bioresources and Bioprocessing
Abstract Bispecific antibodies hold significant potential as next-generation biotherapeutics owing to their ability to simultaneously bind to two targets. However, the development of bispecific antibodies as biotherapeutics has been hindered by the high levels of byproducts produced, including both high molecular weight and low ...
Xiaoyang Wang, Puya Zhao, Min Li, Mengting Li, Huoyan Hong, Kai Gao   +5 more
openaire   +3 more sources

An antibody with Fab-constant domains exchanged for a pair of CH3 domains.

PLoS ONE, 2018
We have designed a complete antibody-like construct where the CH1 and Cκ domains are exchanged for a pair of the CH3 domains and efficient pairing of the heavy and light variable domain is achieved using "Knobs-into-Holes" strategy.
Gordana Wozniak-Knopp, Gerhard Stadlmayr, Jan Walther Perthold, Katharina Stadlbauer, Mathias Gotsmy, Stefan Becker, Florian Rüker   +6 more
doaj   +1 more source