Results 31 to 40 of about 2,068 (144)

Increased yields and biological potency of knob-into-hole-based soluble MHC class II molecules [PDF]

Nature Communications, 2019
AbstractAssembly of soluble peptide-major histocompatibility complex class II (pMHCII) monomers into multimeric structures enables the detection of antigen-specific CD4+ T cells in biological samples and, in some configurations, their reprogramming in vivo.
Pau Serra, Nahir Garabatos, Santiswarup Singha, César Fandos, Josep Garnica, Patricia Solé, Daniel Parras, Jun Yamanouchi, Jesús Blanco, Meritxell Tort, Mireia Ortega, Yang Yang, Kristofor K. Ellestad, Pere Santamaria   +13 more
openaire   +3 more sources

Vector design for enhancing expression level and assembly of knob-into-hole based FabscFv-Fc bispecific antibodies in CHO cells

Antibody Therapeutics, 2022
Abstract Background Two-armed FabscFv-Fc is a favoured bispecific antibody (BsAb) format due to its advantages of the conventional IgG structure. Production of FabscFv-Fc requires expression of three polypeptide chains, one light chain (LC), one heavy chain (HC) and a scFv fused to the Fc (scFvFc) at ...
Han Kee Ong, Ngan T B Nguyen, Jiawu Bi, Yuansheng Yang   +3 more
openaire   +2 more sources

An antibody with Fab-constant domains exchanged for a pair of CH3 domains.

PLoS ONE, 2018
We have designed a complete antibody-like construct where the CH1 and Cκ domains are exchanged for a pair of the CH3 domains and efficient pairing of the heavy and light variable domain is achieved using "Knobs-into-Holes" strategy.
Gordana Wozniak-Knopp, Gerhard Stadlmayr, Jan Walther Perthold, Katharina Stadlbauer, Mathias Gotsmy, Stefan Becker, Florian Rüker   +6 more
doaj   +1 more source

Non-affinity platform for processing knob-into-hole bispecific antibody

Bioresources and Bioprocessing
Abstract Bispecific antibodies hold significant potential as next-generation biotherapeutics owing to their ability to simultaneously bind to two targets. However, the development of bispecific antibodies as biotherapeutics has been hindered by the high levels of byproducts produced, including both high molecular weight and low ...
Xiaoyang Wang, Puya Zhao, Min Li, Mengting Li, Huoyan Hong, Kai Gao   +5 more
openaire   +3 more sources

A lentiviral vector B cell gene therapy platform for the delivery of the anti-HIV-1 eCD4-Ig-knob-in-hole-reversed immunoadhesin

Molecular Therapy - Methods & Clinical Development, 2023
Barriers to effective gene therapy for many diseases include the number of modified target cells required to achieve therapeutic outcomes and host immune responses to expressed therapeutic proteins. As long-lived cells specialized for protein secretion, antibody-secreting B cells are an attractive target for foreign protein expression in blood and ...
Eirini Vamva, Stosh Ozog, Daniel P. Leaman, Rene Yu-Hong Cheng, Nicholas J. Irons, Andee Ott, Claire Stoffers, Iram Khan, Geraldine K.E. Goebrecht, Matthew R. Gardner, Michael Farzan, David J. Rawlings, Michael B. Zwick, Richard G. James, Bruce E. Torbett   +14 more
openaire   +3 more sources

Crystal structures of the HCl salts of pseudopeptidic macrocycles display “knobs into holes” hydrophobic interactions between aliphatic side chains [PDF]

CrystEngComm, 2009
The crystal structures of three HCl salts of pseudopeptidic macrocycles show a H-bonding tubular stack of the rings, where the amino acidic side chains (Val, Leu, Ile) display an inter-columnar “knobs into holes” hydrophobic interaction pattern.
Alfonso Rodríguez, Ignacio, Bolte, Michael, Bru Roig, Miriam, Burguete, M. Isabel, Luis, Santiago V.   +4 more
openaire   +2 more sources

Giant Nonequilibrium Valley Control via Spin‐Selective Hot‐Carrier Transfer in an Antiferromagnetic van der Waals Stack

Advanced Materials, EarlyView.
Spin‐selective hot‐carrier transfer across CrSBr/WSe2/CrSBr interfaces couples the continuously tunable magnetic configuration of antiferromagnetic CrSBr to the valley degrees of freedom of monolayer WSe2, enabling giant nonequilibrium valley control and magnetic‐state‐dependent reversal of circularly polarized emission.
Ke Xiao, Jiabao Yang, Kai Feng, Yicheng Guan, James Caleb Peters, Ramona Hoffmann, Niels Schröter, Stuart S. P. Parkin   +7 more
wiley   +1 more source

Hybridization of Gap Plasmons and Grating‐Coupled Surface Plasmon Polaritons in Metal Nanoparticle Cluster Arrays on a Mirror

Advanced Optical Materials, EarlyView.
Here we use a template‐assisted self‐assembly approach of metal colloids onto metal films to produce hybrid plasmonic metasurfaces that integrate nanoparticle‐on‐mirror cavities with periodic metal colloid arrays. The resulting coupling between gap plasmons and grating‐coupled surface plasmon polaritons enables programmable hybrid modes with tailored ...
Afroditi Koutsogianni, Xiaoyu Qi, Jose Mendoza‐Carreño, Pedro Moronta, Maria Isabel Alonso, David B. Amabilino, Agustín Mihi   +6 more
wiley   +1 more source

Flexoelectricity in Photoconversion: Fundamentals, Materials, and Outlooks

Advanced Science, EarlyView.
Mechanical bending of a flexible cantilever induces a strain gradient in the photoactive material. The resulting flexoelectric field couples with photovoltaic and photoconductive effects, modulating charge generation, separation, and collection. A comparative analysis of oxide perovskites, halide perovskites, and two‐dimensional materials is presented,
Xiang Huang, Feng Li, Rongkun Zheng
wiley   +1 more source

Multiferroic‐Centric Materials and Systems Engineering for Battery Applications: An Insight Into Mechanisms, Strategies, and Characterizations

Advanced Science, EarlyView.
Multiferroic order parameters – polarization, magnetization, and ferroelastic strain – are positioned as dynamic design variables for batteries. Their mechanistic roles, practical tuning through fabrication and external fields, and ferroic‐resolved characterization routes are unified into a closed‐loop framework, revealing how coupled ferroic responses
Jiaqi Su, Yanda Zhu, Hao Peng, Manman Li, Xuzihan Zhang, Meixiao Wu, Siyuan Zhang, Yuhan Zeng, Jiwen Liao, Ming Luo, Hetaishan Huang, Yutong Wang, Sean Li, Wenxian Li   +13 more
wiley   +1 more source